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Structure paper

TitleArchaeal DNA-import apparatus is homologous to bacterial conjugation machinery.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 666, Year 2023
Publish dateFeb 7, 2023
AuthorsLeticia C Beltran / Virginija Cvirkaite-Krupovic / Jessalyn Miller / Fengbin Wang / Mark A B Kreutzberger / Jonasz B Patkowski / Tiago R D Costa / Stefan Schouten / Ilya Levental / Vincent P Conticello / Edward H Egelman / Mart Krupovic /
PubMed AbstractConjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient ...Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two from hyperthermophilic archaea (Aeropyrum pernix and Pyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacterium Agrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been 'domesticated', that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA.
External linksNat Commun / PubMed:36750723 / PubMed Central
MethodsEM (helical sym.)
Resolution3.44 - 4.1 Å
Structure data

27413

8dft

EMDB-27413, PDB-8dft:
Cryo-EM structure of conjugative pili from Pyrobaculum calidifontis
Method: EM (helical sym.) / Resolution: 4.1 Å

27414

8dfu

EMDB-27414, PDB-8dfu:
Cryo-EM structure of conjugation pili from Aeropyrum pernix
Method: EM (helical sym.) / Resolution: 3.44 Å

28657

8exh

EMDB-28657, PDB-8exh:
Agrobacterium tumefaciens Tpilus
Method: EM (helical sym.) / Resolution: 3.5 Å

Chemicals

ChemComp-TT0:
[(2~{S},7~{S},11~{S},15~{S},19~{R},22~{R},26~{S},30~{R},34~{R},38~{S},43~{S},47~{S},51~{S},55~{R},58~{R},62~{S},66~{R},70~{R})-38-(hydroxymethyl)-7,11,15,19,22,26,30,34,43,47,51,55,58,62,66,70-hexadecamethyl-1,4,37,40-tetraoxacyclodoheptacont-2-yl]methanol

ChemComp-RHR:
(2S)-3-{[(3R,7S,11S,15S)-3,7,11,15,19-pentamethylicosyl]oxy}-2-{[(2R,6S,10S,14R)-2,6,10,14,18-pentamethylnonadecyl]oxy}propyl dihydrogen phosphate

ChemComp-X3D:
(14S,17R)-20-amino-17-hydroxy-11,17-dioxo-12,16,18-trioxa-17lambda~5~-phosphaicosan-14-yl tetradecanoate

Source
  • pyrobaculum calidifontis (archaea)
  • aeropyrum pernix (archaea)
  • Agrobacterium fabrum str. C58 (bacteria)
  • agrobacterium fabrum (strain c58 / atcc 33970) (bacteria)
KeywordsPROTEIN FIBRIL / archaeal conjugative pilus / Archaeal conjugation pilus / T-pilus

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