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Title | Toward structural-omics of the bovine retinal pigment epithelium. |
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Journal, issue, pages | Cell Rep, Vol. 41, Issue 13, Page 111876, Year 2022 |
Publish date | Dec 27, 2022 |
Authors | Christopher E Morgan / Zhemin Zhang / Masaru Miyagi / Marcin Golczak / Edward W Yu / |
PubMed Abstract | The use of an integrated systems biology approach to investigate tissues and organs has been thought to be impracticable in the field of structural biology, where the techniques mainly focus on ...The use of an integrated systems biology approach to investigate tissues and organs has been thought to be impracticable in the field of structural biology, where the techniques mainly focus on determining the structure of a particular biomacromolecule of interest. Here, we report the use of cryoelectron microscopy (cryo-EM) to define the composition of a raw bovine retinal pigment epithelium (RPE) lysate. From this sample, we simultaneously identify and solve cryo-EM structures of seven different RPE enzymes whose functions affect neurotransmitter recycling, iron metabolism, gluconeogenesis, glycolysis, axonal development, and energy homeostasis. Interestingly, dysfunction of these important proteins has been directly linked to several neurodegenerative disorders, including Huntington's disease, amyotrophic lateral sclerosis (ALS), Parkinson's disease, Alzheimer's disease, and schizophrenia. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level. |
External links | Cell Rep / PubMed:36577381 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.28 - 3.32 Å |
Structure data | EMDB-26350, PDB-7u5h: EMDB-26351, PDB-7u5i: EMDB-26352, PDB-7u5j: EMDB-26353, PDB-7u5k: EMDB-26354, PDB-7u5l: EMDB-26355, PDB-7u5m: EMDB-26356, PDB-7u5n: |
Chemicals | ChemComp-ZN: ChemComp-HOH: ChemComp-FE: ChemComp-NAD: ChemComp-MN: |
Source |
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Keywords | PEPTIDE BINDING PROTEIN / DNPEP / aminopeptidase / tetrahedral Symmetry / TRANSFERASE / CK / creatine kinase / mitochondrial / LYASE / ALDOA / fructose-bisphosphate aldolase / aldolase / NEUROPEPTIDE / DPYSL2 / dihydropyrimidinase-related protein 2 / METAL BINDING PROTEIN / ferritin / FTH1 / GAPDH / glyceraldehyde-3-phosphate dehydrogenase / GS / glutamine synthetase / GLUL |