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Structure paper

TitleStructures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3830, Year 2020
Publish dateJul 31, 2020
AuthorsRavi Kiran Koripella / Manjuli R Sharma / Kalpana Bhargava / Partha P Datta / Prem S Kaushal / Pooja Keshavan / Linda L Spremulli / Nilesh K Banavali / Rajendra K Agrawal /
PubMed AbstractThe mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we ...The mammalian mitochondrial ribosome (mitoribosome) and its associated translational factors have evolved to accommodate greater participation of proteins in mitochondrial translation. Here we present the 2.68-3.96 Å cryo-EM structures of the human 55S mitoribosome in complex with the human mitochondrial elongation factor G1 (EF-G1) in three distinct conformational states, including an intermediate state and a post-translocational state. These structures reveal the role of several mitochondria-specific (mito-specific) mitoribosomal proteins (MRPs) and a mito-specific segment of EF-G1 in mitochondrial tRNA (tRNA) translocation. In particular, the mito-specific C-terminal extension in EF-G1 is directly involved in translocation of the acceptor arm of the A-site tRNA. In addition to the ratchet-like and independent head-swiveling motions exhibited by the small mitoribosomal subunit, we discover significant conformational changes in MRP mL45 at the nascent polypeptide-exit site within the large mitoribosomal subunit that could be critical for tethering of the elongating mitoribosome onto the inner-mitochondrial membrane.
External linksNat Commun / PubMed:32737313 / PubMed Central
MethodsEM (single particle)
Resolution2.96 - 6.8 Å
Structure data

21233

6vlz

EMDB-21233, PDB-6vlz:
Structure of the human mitochondrial ribosome-EF-G1 complex (ClassI)
Method: EM (single particle) / Resolution: 2.97 Å

21242

6vmi

EMDB-21242, PDB-6vmi:
Structure of the human mitochondrial ribosome-EF-G1 complex (ClassIII)
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-22209:
Structure of bovine 55S mitochondrial ribosome
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-22212:
Structure of the human mitochondrial ribosome-EF-G1 complex (ClassII)
Method: EM (single particle) / Resolution: 3.96 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

Source
  • homo sapiens (human)
  • Bos taurus (cattle)
KeywordsRIBOSOME / mitochondrial elongation factor-G1 / 55S ribosome

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