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-Structure paper
Title | Cryo-EM structures provide insight into how E. coli FF ATP synthase accommodates symmetry mismatch. |
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Journal, issue, pages | Nat Commun, Vol. 11, Issue 1, Page 2615, Year 2020 |
Publish date | May 26, 2020 |
Authors | Meghna Sobti / James L Walshe / Di Wu / Robert Ishmukhametov / Yi C Zeng / Carol V Robinson / Richard M Berry / Alastair G Stewart / |
PubMed Abstract | FF ATP synthase functions as a biological rotary generator that makes a major contribution to cellular energy production. It comprises two molecular motors coupled together by a central and a ...FF ATP synthase functions as a biological rotary generator that makes a major contribution to cellular energy production. It comprises two molecular motors coupled together by a central and a peripheral stalk. Proton flow through the F motor generates rotation of the central stalk, inducing conformational changes in the F motor that catalyzes ATP production. Here we present nine cryo-EM structures of E. coli ATP synthase to 3.1-3.4 Å resolution, in four discrete rotational sub-states, which provide a comprehensive structural model for this widely studied bacterial molecular machine. We observe torsional flexing of the entire complex and a rotational sub-step of F associated with long-range conformational changes that indicates how this flexibility accommodates the mismatch between the 3- and 10-fold symmetries of the F and F motors. We also identify density likely corresponding to lipid molecules that may contribute to the rotor/stator interaction within the F motor. |
External links | Nat Commun / PubMed:32457314 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 3.4 Å |
Structure data | EMDB-20167, PDB-6oqr: EMDB-20168, PDB-6oqs: EMDB-20169, PDB-6oqt: EMDB-20170, PDB-6oqu: EMDB-20171, PDB-6oqv: EMDB-20172, PDB-6oqw: EMDB-20454, PDB-6pqv: EMDB-21419, PDB-6vwk: EMDB-21854, PDB-6wnq: EMDB-21855, PDB-6wnr: |
Chemicals | ChemComp-ATP: ChemComp-MG: ChemComp-ADP: ChemComp-PO4: |
Source |
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Keywords | MEMBRANE PROTEIN / E coli ATP Synthase / ion channel / ATPase / F1Fo ATP synthase |