Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural specializations of the sperm tail.
Journal, issue, pages	Cell, Vol. 186, Issue 13, Page 2880-2896.e17, Year 2023
Publish date	Jun 22, 2023
Authors	Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai /
PubMed Abstract	Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.
External links	Cell / PubMed:37327785 / PubMed Central
Methods	EM (single particle)
Resolution	3.3 - 4.1 Å
Structure data	17187 8otz EMDB-17187, PDB-8otz: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm Method: EM (single particle) / Resolution: 3.6 Å 17188 8ou0 EMDB-17188, PDB-8ou0: bovine sperm endpiece singlet microtubules (one tubulin dimer and associated microtubule inner proteins) Method: EM (single particle) / Resolution: 3.5 Å EMDB-18071: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen top Method: EM (single particle) / Resolution: 3.6 Å EMDB-18072: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen middle Method: EM (single particle) / Resolution: 3.5 Å EMDB-18073: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen bottom Method: EM (single particle) / Resolution: 3.6 Å EMDB-18074: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 top Method: EM (single particle) / Resolution: 3.5 Å EMDB-18075: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 middle Method: EM (single particle) / Resolution: 3.4 Å EMDB-18076: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 bottom Method: EM (single particle) / Resolution: 3.5 Å EMDB-18077: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 top Method: EM (single particle) / Resolution: 3.7 Å EMDB-18078: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 middle Method: EM (single particle) / Resolution: 3.6 Å EMDB-18079: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 bottom Method: EM (single particle) / Resolution: 3.7 Å EMDB-18080: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 top Method: EM (single particle) / Resolution: 3.8 Å EMDB-18081: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 middle Method: EM (single particle) / Resolution: 3.7 Å EMDB-18082: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 bottom Method: EM (single particle) / Resolution: 3.8 Å EMDB-18083: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 top Method: EM (single particle) / Resolution: 3.9 Å EMDB-18084: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 middle Method: EM (single particle) / Resolution: 3.8 Å EMDB-18085: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 bottom Method: EM (single particle) / Resolution: 3.8 Å EMDB-18086: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction top Method: EM (single particle) / Resolution: 3.6 Å EMDB-18087: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction middle Method: EM (single particle) / Resolution: 3.5 Å EMDB-18088: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction bottom Method: EM (single particle) / Resolution: 3.6 Å EMDB-18089: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon top Method: EM (single particle) / Resolution: 3.5 Å EMDB-18090: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon middle Method: EM (single particle) / Resolution: 3.4 Å EMDB-18091: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon bottom Method: EM (single particle) / Resolution: 3.4 Å EMDB-18092: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction top Method: EM (single particle) / Resolution: 3.7 Å EMDB-18093: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction middle Method: EM (single particle) / Resolution: 3.6 Å EMDB-18094: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction bottom Method: EM (single particle) / Resolution: 3.7 Å EMDB-18095: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 top Method: EM (single particle) / Resolution: 3.9 Å EMDB-18096: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 middle Method: EM (single particle) / Resolution: 3.8 Å EMDB-18097: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 bottom Method: EM (single particle) / Resolution: 3.8 Å EMDB-18098: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 top Method: EM (single particle) / Resolution: 4.0 Å EMDB-18099: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 middle Method: EM (single particle) / Resolution: 3.9 Å EMDB-18100: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 bottom Method: EM (single particle) / Resolution: 4.0 Å EMDB-18101: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 top Method: EM (single particle) / Resolution: 4.1 Å EMDB-18102: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 middle Method: EM (single particle) / Resolution: 4.1 Å EMDB-18103: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 bottom Method: EM (single particle) / Resolution: 4.1 Å EMDB-18104: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 top Method: EM (single particle) / Resolution: 4.1 Å EMDB-18105: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 middle Method: EM (single particle) / Resolution: 4.0 Å EMDB-18106: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 bottom Method: EM (single particle) / Resolution: 4.0 Å EMDB-18107: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, consensus refinement Method: EM (single particle) / Resolution: 4.0 Å 40619 8snb EMDB-40619: cryo-EM structure of sea urchin sperm doublet microtubule (48-nm periodicity) PDB-8snb: atomic model of sea urchin sperm doublet microtubule (48-nm periodicity) Method: EM (single particle) / Resolution: 3.3 Å EMDB-40620: cryo-EM density map of the 48-nm repeat doublet microtubule with beak from Chlamydomonas Method: EM (single particle) / Resolution: 3.8 Å EMDB-40621: cryo-EM density map of the 48-nm repeat doublet microtubule without beak from Chlamydomonas Method: EM (single particle) / Resolution: 3.6 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM
Source	bos taurus (cattle) strongylocentrotus purpuratus (purple sea urchin) Chlamydomonas reinhardtii (plant)
Keywords	STRUCTURAL PROTEIN / microtubule / cilia / sperm / axoneme / CYTOSOLIC PROTEIN / microtubule inner protein / doublet microtubule / DMT / flagella / FAP / CFAP / MIP / MAP / cytoskeleton / tublin / sea urchin / TEX / Tektin / SAXO / ODF / SPATA / RIB