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Title | From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y. |
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Journal, issue, pages | Commun Chem, Vol. 7, Issue 1, Page 14, Year 2024 |
Publish date | Jan 17, 2024 |
Authors | Luka Kavčič / Andreja Kežar / Neža Koritnik / Magda Tušek Žnidarič / Tajda Klobučar / Žiga Vičič / Franci Merzel / Ellie Holden / Justin L P Benesch / Marjetka Podobnik / |
PubMed Abstract | The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid ...The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid nanoparticles. However, the potential of CPs of the wide-spread flexuous filamentous plant viruses remains to be explored. Here, we show that we can control the shape, size, RNA encapsidation ability, symmetry, stability and surface functionalization of nanoparticles through structure-based design of CP from potato virus Y (PVY). We provide high-resolution insight into CP-based self-assemblies, ranging from large polymorphic or monomorphic filaments to smaller annular, cubic or spherical particles. Furthermore, we show that we can prevent CP self-assembly in bacteria by fusion with a cleavable protein, enabling controlled nanoparticle formation in vitro. Understanding the remarkable structural diversity of PVY CP not only provides possibilities for the production of biodegradable nanoparticles, but may also advance future studies of CP's polymorphism in a biological context. |
External links | Commun Chem / PubMed:38233506 / PubMed Central |
Methods | EM (helical sym.) / EM (single particle) |
Resolution | 2.41 - 12.75 Å |
Structure data | EMDB-17046, PDB-8opa: EMDB-17047, PDB-8opb: EMDB-17048, PDB-8opc: EMDB-17049, PDB-8opd: EMDB-17050, PDB-8ope: EMDB-17051, PDB-8opf: EMDB-17052, PDB-8opg: EMDB-17053, PDB-8oph: EMDB-17054: RNA-free helical assembly from truncated PVY coat protein EMDB-17055: Stacked-ring assembly from truncated PVY coat protein EMDB-17056: Stacked-ring assembly from truncated PVY coat protein with L99C mutation EMDB-17057: Helical assembly from truncated PVY coat protein with L99C mutation EMDB-17058: Helical assembly from truncated PVY coat protein with K153E mutation EMDB-17059: Cross-shaped junction assembly from truncated PVY coat protein with E150C mutation EMDB-17060: Global refinement map of head-to-head double ring assembly from truncated PVY coat protein with K176E mutation EMDB-17061: Local refinement map of head-to-head double ring assembly from truncated PVY coat protein with K176E mutation EMDB-17062, PDB-8opj: EMDB-17063, PDB-8opk: EMDB-17064: Spherical assembly from truncated PVY coat protein with K177E mutation EMDB-17065: Cubic assembly from truncated PVY coat protein with K176C mutation and removed His tag EMDB-17066: Virus-like Particle based on PVY coat protein with L99C and K176C mutation with stacked-ring architecture EMDB-17067: Virus-like Particle based on PVY coat protein with L99C and K176C mutation with RNA-free helical architecture EMDB-17068: Virus-like Particle based on PVY coat protein with E150C and G193C mutation with stacked-ring architecture EMDB-17069: Virus-like Particle based on PVY coat protein with E150C and G193C mutation with RNA-free helical architecture EMDB-17070: Virus-like Particle based on PVY coat protein with S39C and E72C mutation with stacked-ring architecture EMDB-17071: Virus-like Particle based on PVY coat protein with S39C and E72C mutation with RNA-free helical architecture EMDB-17072, PDB-8opl: EMDB-17073: Virus-like Particle based on PVY coat protein with D136C mutation with helical architecture encapsidating ssRNA EMDB-17074: Virus-like Particle based on PVY coat protein with R46A mutation with helical architecture encapsidating ssRNA EMDB-17075: Virus-like Particle based on PVY coat protein with E139A mutation with helical architecture encapsidating ssRNA |
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Keywords | VIRUS LIKE PARTICLE / stacked-ring / VLP / Potyvirus / PVY / helical / RNA-free / ssRNA / dC40 / dC60 / dC79 / H2T double ring / trCP / cubes / K176C |