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Structure paper

TitleStructural landscape of the respiratory syncytial virus nucleocapsids.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5732, Year 2023
Publish dateSep 15, 2023
AuthorsLorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche /
PubMed AbstractHuman Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation.
External linksNat Commun / PubMed:37714861 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution2.8 - 6.2 Å
Structure data

EMDB-17030: Helical nucleocapsid of the Respiratory Syncytial Virus
Method: EM (helical sym.) / Resolution: 6.2 Å

17031

8oou

EMDB-17031, PDB-8oou:
Double-ring nucleocapsid of the Respiratory Syncytial Virus
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-17034: Helical nucleocapsid of the N1-370 mutant of the human Respiratory Syncytial Virus
Method: EM (helical sym.) / Resolution: 4.3 Å

17035

8op1

EMDB-17035, PDB-8op1:
Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-17036: Double-headed nucleocapsid of the human Respiratory Syncytial Virus
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-17037: Ring-capped nucleocapsid of the Respiratory Syncytial Virus
Method: EM (single particle) / Resolution: 3.9 Å

17038

8op2

EMDB-17038, PDB-8op2:
Stacks of nucleocapsid rings of the N1-370 mutant of the human Respiratory Syncytial Virus
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • human respiratory syncytial virus a strain long
  • respiratory syncytial virus
  • trichoplusia ni (cabbage looper)
KeywordsVIRAL PROTEIN / D10-symmetry / N-RNA / nucleoprotein / RSV / nucleocapsid

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