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-Structure paper
Title | Direct cell extraction of membrane proteins for structure-function analysis. |
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Journal, issue, pages | Sci Rep, Vol. 13, Issue 1, Page 1420, Year 2023 |
Publish date | Jan 25, 2023 |
Authors | Ieva Drulyte / Aspen Rene Gutgsell / Pilar Lloris-Garcerá / Michael Liss / Stefan Geschwindner / Mazdak Radjainia / Jens Frauenfeld / Robin Löving / |
PubMed Abstract | Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach ...Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM. |
External links | Sci Rep / PubMed:36697499 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 Å |
Structure data | EMDB-15110, PDB-8a3b: |
Source |
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Keywords | MEMBRANE PROTEIN / Membrane transporter / ATP-release channel / inflammation / immune function |