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TitleStructural Insights into Iron Ions Accumulation in Dps Nanocage.
Journal, issue, pagesInt J Mol Sci, Vol. 23, Issue 10, Year 2022
Publish dateMay 10, 2022
AuthorsYury Chesnokov / Andrey Mozhaev / Roman Kamyshinsky / Alexander Gordienko / Liubov Dadinova /
PubMed AbstractDps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. ...Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate clusters of ferric ions in its nanocages, thus providing the bacterium with physical and chemical protection. Here, cryo-electron microscopy was used to study the accumulation of iron ions in the nanocage of a Dps protein from . We demonstrate that Fe concentration in the solution and incubation time have an insignificant effect on the volume and the morphology of iron minerals formed in Dps nanocages. However, an increase in the Fe level leads to an increase in the proportion of larger clusters and the clusters themselves are composed of discrete ~1-1.5 nm subunits.
External linksInt J Mol Sci / PubMed:35628121 / PubMed Central
MethodsEM (single particle)
Resolution2.59 - 14.5 Å
Structure data

EMDB-14943: Structure of DPS
Method: EM (single particle) / Resolution: 2.59 Å

EMDB-14948: Dps nanocage with the "small circle" mineral core
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-14949: Dps nanocage with the "dumbbell" mineral core
Method: EM (single particle) / Resolution: 10.9 Å

EMDB-14950: Dps nanocage with a medium mineral core
Method: EM (single particle) / Resolution: 10.8 Å

EMDB-14951: Dps nanocage with a large mineral core
Method: EM (single particle) / Resolution: 14.5 Å

EMDB-14952: Dps nanocage with the symmetrical hollow mineral core
Method: EM (single particle) / Resolution: 6.7 Å

Source
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)

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