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-Structure paper
Title | A modular platform for automated cryo-FIB workflows. |
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Journal, issue, pages | Elife, Vol. 10, Year 2021 |
Publish date | Dec 24, 2021 |
Authors | Sven Klumpe / Herman Kh Fung / Sara K Goetz / Ievgeniia Zagoriy / Bernhard Hampoelz / Xiaojie Zhang / Philipp S Erdmann / Janina Baumbach / Christoph W Müller / Martin Beck / Jürgen M Plitzko / Julia Mahamid / |
PubMed Abstract | Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ...Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga ion beam currents required to maintain the structural integrity of vitreous specimens, common preparation protocols are time-consuming and labor intensive. The improved stability of new-generation cryo-FIB instruments now enables automated operations. Here, we present an open-source software tool, SerialFIB, for creating automated and customizable cryo-FIB preparation protocols. The software encompasses a graphical user interface for easy execution of routine lamellae preparations, a scripting module compatible with available Python packages, and interfaces with three-dimensional correlative light and electron microscopy (CLEM) tools. SerialFIB enables the streamlining of advanced cryo-FIB protocols such as multi-modal imaging, CLEM-guided lamella preparation and in situ lamella lift-out procedures. Our software therefore provides a foundation for further development of advanced cryogenic imaging and sample preparation protocols. |
External links | Elife / PubMed:34951584 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 20.8 - 24.0 Å |
Structure data | EMDB-13832: EMDB-13833: EMDB-13834: EMDB-13835: EMDB-13836: EMDB-13837: EMDB-13838: EMDB-13878: EMDB-13879: |
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