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TitleStructure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.
Journal, issue, pagesMol Cell, Vol. 81, Issue 2, Page 293-303.e4, Year 2021
Publish dateJan 21, 2021
AuthorsBenjamin Lau / Jingdong Cheng / Dirk Flemming / Giuseppe La Venuta / Otto Berninghausen / Roland Beckmann / Ed Hurt /
PubMed AbstractRibosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in ...Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension at the 18S rRNA that is integrated into the huge 90S pre-ribosome structure. Upon endo-nucleolytic cleavage at an internal site, A, the 5'-ETS is separated from the 18S rRNA and degraded. Here we present biochemical and cryo-electron microscopy analyses that depict the RNA exosome, a major 3'-5' exoribonuclease complex, in a super-complex with the 90S pre-ribosome. The exosome is docked to the 90S through its co-factor Mtr4 helicase, a processive RNA duplex-dismantling helicase, which strategically positions the exosome at the base of 5'-ETS helices H9-H9', which are dislodged in our 90S-exosome structures. These findings suggest a direct role of the exosome in structural remodeling of the 90S pre-ribosome to drive eukaryotic ribosome synthesis.
External linksMol Cell / PubMed:33326748
MethodsEM (single particle)
Resolution3.8 - 7.5 Å
Structure data

11807

7ajt

EMDB-11807, PDB-7ajt:
Cryo-EM structure of the 90S-exosome super-complex (state Pre-A1-exosome)
Method: EM (single particle) / Resolution: 4.6 Å

11808

7aju

EMDB-11808, PDB-7aju:
Cryo-EM structure of the 90S-exosome super-complex (state Post-A1-exosome)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-11809:
Cryo-EM structure of the 90S-exosome super-complex (state Post-A1-exosome, Dhr1-Dim1)
Method: EM (single particle) / Resolution: 7.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • Saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / Ribosome biogenesis / Exosome / 90S pre-ribosome / A1 cleavage / Mtr4

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