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TitleFree fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein.
Journal, issue, pagesScience, Vol. 370, Issue 6517, Page 725-730, Year 2020
Publish dateNov 6, 2020
AuthorsChristine Toelzer / Kapil Gupta / Sathish K N Yadav / Ufuk Borucu / Andrew D Davidson / Maia Kavanagh Williamson / Deborah K Shoemark / Frederic Garzoni / Oskar Staufer / Rachel Milligan / Julien Capin / Adrian J Mulholland / Joachim Spatz / Daniel Fitzgerald / Imre Berger / Christiane Schaffitzel /
PubMed AbstractCoronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), represents a global crisis. Key to SARS-CoV-2 therapeutic development is unraveling the ...Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), represents a global crisis. Key to SARS-CoV-2 therapeutic development is unraveling the mechanisms that drive high infectivity, broad tissue tropism, and severe pathology. Our 2.85-angstrom cryo-electron microscopy structure of SARS-CoV-2 spike (S) glycoprotein reveals that the receptor binding domains tightly bind the essential free fatty acid linoleic acid (LA) in three composite binding pockets. A similar pocket also appears to be present in the highly pathogenic severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). LA binding stabilizes a locked S conformation, resulting in reduced angiotensin-converting enzyme 2 (ACE2) interaction in vitro In human cells, LA supplementation synergizes with the COVID-19 drug remdesivir, suppressing SARS-CoV-2 replication. Our structure directly links LA and S, setting the stage for intervention strategies that target LA binding by SARS-CoV-2.
External linksScience / PubMed:32958580 / PubMed Central
MethodsEM (single particle)
Resolution2.85 - 3.55 Å
Structure data

11144

6zb4

EMDB-11144, PDB-6zb4:
SARS CoV-2 Spike protein, Closed conformation, C1 symmetry
Method: EM (single particle) / Resolution: 3.03 Å

11145

6zb5

EMDB-11145, PDB-6zb5:
SARS CoV-2 Spike protein, Closed conformation, C3 symmetry
Method: EM (single particle) / Resolution: 2.85 Å

EMDB-11146:
SARS CoV-2 Spike protein, Open conformation
Method: EM (single particle) / Resolution: 3.55 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-EIC:
LINOLEIC ACID

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / SARS-Cov2 / COVID / Linoleic Acid / Spike

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