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Structure paper

TitleDual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.
Journal, issue, pagesCell, Vol. 183, Issue 2, Page 457-473.e20, Year 2020
Publish dateOct 15, 2020
AuthorsMirkko Flecken / Huping Wang / Leonhard Popilka / F Ulrich Hartl / Andreas Bracher / Manajit Hayer-Hartl /
PubMed AbstractRubisco, the key enzyme of CO fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone ...Rubisco, the key enzyme of CO fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C terminus of the adjacent RbcL opens the catalytic site for inhibitor release. An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. The cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization.
External linksCell / PubMed:32979320
MethodsEM (single particle) / X-ray diffraction
Resolution1.38 - 8.2 Å
Structure data

11028

6z1f

EMDB-11028, PDB-6z1f:
CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120)
Method: EM (single particle) / Resolution: 2.86 Å

11029

6z1g

EMDB-11029, PDB-6z1g:
CryoEM structure of the interaction between Rubisco Activase small-subunit-like (SSUL) domain with Rubisco from Nostoc sp. (strain PCC7120)
Method: EM (single particle) / Resolution: 8.2 Å

EMDB-11575:
CryoEM Local map of Rubisco Activase from the complex with its substrate Rubisco from Nostoc sp. (strain PCC7120)
Method: EM (single particle) / Resolution: 3.29 Å

PDB-6has:
Crystal Structure of the small subunit-like domain of Rubisco activase from Nostoc sp. (strain PCC 7120)
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

PDB-6z1d:
Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120), Gadolinium complex
Method: X-RAY DIFFRACTION / Resolution: 2.705 Å

PDB-6z1e:
Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)
Method: X-RAY DIFFRACTION / Resolution: 2.454 Å

Chemicals

ChemComp-NI:
NICKEL (II) ION

ChemComp-HOH:
WATER

ChemComp-GD:
GADOLINIUM ATOM

ChemComp-CL:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-CAP:
2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

Source
  • nostoc sp. pcc 7120 = fachb-418 (bacteria)
  • nostoc sp. (strain pcc 7120 / sag 25.82 / utex 2576) (bacteria)
KeywordsCHAPERONE / alpha-beta structure / Rubisco / AAA+ domain / AAA+ / beta barrel / PHOTOSYNTHESIS

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