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-Structure paper
Title | Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism. |
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Journal, issue, pages | Nat Struct Mol Biol, Vol. 27, Issue 8, Page 743-751, Year 2020 |
Publish date | Jul 13, 2020 |
Authors | Byung-Gil Lee / Fabian Merkel / Matteo Allegretti / Markus Hassler / Christopher Cawood / Léa Lecomte / Francis J O'Reilly / Ludwig R Sinn / Pilar Gutierrez-Escribano / Marc Kschonsak / Sol Bravo / Takanori Nakane / Juri Rappsilber / Luis Aragon / Martin Beck / Jan Löwe / Christian H Haering / |
PubMed Abstract | Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The ...Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large loop domains, although the underlying molecular mechanisms have remained unknown. We used cryo-EM to investigate the nucleotide-driven reaction cycle of condensin from the budding yeast Saccharomyces cerevisiae. Our structures of the five-subunit condensin holo complex at different functional stages suggest that ATP binding induces the transition of the SMC coiled coils from a folded-rod conformation into a more open architecture. ATP binding simultaneously triggers the exchange of the two HEAT-repeat subunits bound to the SMC ATPase head domains. We propose that these steps result in the interconversion of DNA-binding sites in the catalytic core of condensin, forming the basis of the DNA translocation and loop-extrusion activities. |
External links | Nat Struct Mol Biol / PubMed:32661420 / PubMed Central |
Methods | EM (single particle) |
Resolution | 4.2 - 9.1 Å |
Structure data | EMDB-10944, PDB-6yvd: EMDB-10947: EMDB-10948: EMDB-10951: Condensin complex from S.cerevisiae ATP-free apo non-engaged state: overall map EMDB-10952: Condensin complex from S.cerevisiae ATP-free apo bridged state: focused refinement on head segment EMDB-10953: EMDB-10954: EMDB-10964: |
Source |
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Keywords | CELL CYCLE / Condensin chromosome condensation SMC protein / essential for the functional organization of genomes |