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-Structure paper
Title | Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts. |
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Journal, issue, pages | PLoS Pathog, Vol. 16, Issue 12, Page e1009146, Year 2020 |
Publish date | Dec 28, 2020 |
Authors | Rebecca Chandler-Bostock / Carlos P Mata / Richard J Bingham / Eric C Dykeman / Bo Meng / Tobias J Tuthill / David J Rowlands / Neil A Ranson / Reidun Twarock / Peter G Stockley / |
PubMed Abstract | Picornaviruses are important viral pathogens, but despite extensive study, the assembly process of their infectious virions is still incompletely understood, preventing the development of anti-viral ...Picornaviruses are important viral pathogens, but despite extensive study, the assembly process of their infectious virions is still incompletely understood, preventing the development of anti-viral strategies targeting this essential part of the life cycle. We report the identification, via RNA SELEX and bioinformatics, of multiple RNA sites across the genome of a typical enterovirus, enterovirus-E (EV-E), that each have affinity for the cognate viral capsid protein (CP) capsomer. Many of these sites are evolutionarily conserved across known EV-E variants, suggesting they play essential functional roles. Cryo-electron microscopy was used to reconstruct the EV-E particle at ~2.2 Å resolution, revealing extensive density for the genomic RNA. Relaxing the imposed symmetry within the reconstructed particles reveals multiple RNA-CP contacts, a first for any picornavirus. Conservative mutagenesis of the individual RNA-contacting amino acid side chains in EV-E, many of which are conserved across the enterovirus family including poliovirus, is lethal but does not interfere with replication or translation. Anti-EV-E and anti-poliovirus aptamers share sequence similarities with sites distributed across the poliovirus genome. These data are consistent with the hypothesis that these RNA-CP contacts are RNA Packaging Signals (PSs) that play vital roles in assembly and suggest that the RNA PSs are evolutionarily conserved between pathogens within the family, augmenting the current protein-only assembly paradigm for this family of viruses. |
External links | PLoS Pathog / PubMed:33370422 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.23 - 2.6 Å |
Structure data | EMDB-10504, PDB-6thd: EMDB-10505: EMDB-10506, PDB-6thn: |
Chemicals | ChemComp-MYR: ChemComp-SO4: ChemComp-HOH: |
Source |
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Keywords | VIRUS / BEV1 / enterovirus / picornavirus / RNA |