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Title | Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. |
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Journal, issue, pages | Nat Commun, Vol. 10, Issue 1, Page 2386, Year 2019 |
Publish date | Jun 3, 2019 |
![]() | Xiao Fan / Jia Wang / Xing Zhang / Zi Yang / Jin-Can Zhang / Lingyun Zhao / Hai-Lin Peng / Jianlin Lei / Hong-Wei Wang / ![]() |
PubMed Abstract | The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than ...The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa. |
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Methods | EM (single particle) |
Resolution | 3.2 - 3.3 Å |
Structure data | |
Chemicals | ![]() ChemComp-BTN: ![]() ChemComp-HOH: |
Source |
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![]() | CYTOSOLIC PROTEIN / streptavidin |