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Structure paper

TitletRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.
Journal, issue, pagesCell Rep, Vol. 25, Issue 10, Page 2676-22688.e7, Year 2018
Publish dateDec 4, 2018
AuthorsJulia Flis / Mikael Holm / Emily J Rundlet / Justus Loerke / Tarek Hilal / Marylena Dabrowski / Jörg Bürger / Thorsten Mielke / Scott C Blanchard / Christian M T Spahn / Tatyana V Budkevich /
PubMed AbstractTranslocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes ...Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site.
External linksCell Rep / PubMed:30517857 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.6 Å
Structure data

0098

6gz3

EMDB-0098, PDB-6gz3:
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1)
Method: EM (single particle) / Resolution: 3.6 Å

0099

6gz4

EMDB-0099, PDB-6gz4:
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)
Method: EM (single particle) / Resolution: 3.6 Å

0100

6gz5

EMDB-0100, PDB-6gz5:
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-3 (TI-POST-3)
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Source
  • oryctolagus cuniculus (rabbit)
  • enterobacteria phage sp6 (virus)
  • saccharomyces cerevisiae (brewer's yeast)
  • rabbit (rabbit)
  • baker's yeast (brewer's yeast)
KeywordsRIBOSOME / translocation rabbit ribosome / 80S / eEF2 / head swivel / rotation / translocation / mammalian / EF-G / GMPPNP / DDE / Diphthamide

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