Title | Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome |
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Journal, issue, pages | Nat. Struct. Mol. Biol., Year 2024 |
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Publish date | Oct 23, 2023 (structure data deposition date) |
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Authors | Molodtsov V / Wang C / Zhang J / Kaelber JT / Blaha G / Ebright RH |
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External links | Search PubMed |
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Methods | EM (single particle) |
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Resolution | 3.2 - 6.6 Å |
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Structure data | EMDB-42453, PDB-8upo: Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 5.5 Å EMDB-42454, PDB-8upr: Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 5.3 Å EMDB-42473, PDB-8uql: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 3.2 Å EMDB-42474, PDB-8uqm: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 5.3 Å EMDB-42477, PDB-8uqp: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 3.8 Å EMDB-42479, PDB-8ur0: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 3.4 Å EMDB-42492, PDB-8urh: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 5.7 Å EMDB-42493, PDB-8uri: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 5.3 Å EMDB-42503, PDB-8urx: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Method: EM (single particle) / Resolution: 6.6 Å |
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Chemicals | |
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Source | ![](img/tx_bacteria.gif) escherichia coli (E. coli)- synthetic construct (others)
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Keywords | TRANSCRIPTION / translation / RfaH / gene expression / regulation / ops / ribosome / coupling |
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