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Structure paper

TitleStructural dissection of alpha beta-tubulin heterodimer assembly and disassembly by human tubulin-specific chaperones
Journal, issue, pagesScience, Year 2025
Publish dateFeb 6, 2025 (structure data deposition date)
AuthorsSeong YJ / Kim HM / Byun KM / Park YW / Roh SH
External linksScience / Search PubMed
MethodsEM (single particle)
Resolution2.14 - 3.59 Å
Structure data

EMDB-63376: Consensus map of the TBC-DE-Arl2-beta-tubulin complex with GTP
Method: EM (single particle) / Resolution: 2.45 Å

EMDB-63377: Local refinement map of Arl2 in the TBC-DE-Arl2-beta-tubulin-GTP complex
Method: EM (single particle) / Resolution: 2.56 Å

EMDB-63378: Local refinement map of AR3 domain in the TBC-DE-Arl2-beta-tubulin-GTP complex
Method: EM (single particle) / Resolution: 2.41 Å

EMDB-63546: Consensus map of the TBC-DE-Arl2-alpha-beta-tubulin complex with GTP
Method: EM (single particle) / Resolution: 2.55 Å

EMDB-63547: Local refinement map of TBCE and alpha-tubulin in the TBC-DE-Arl2-alpha-beta-tubulin-GTP complex
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-63548: Local refinement map of Arl2 and TBCD-AR1 domain in the TBC-DE-Arl2-alpha-beta-tubulin-GTP complex
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-63549: Consensus map of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx
Method: EM (single particle) / Resolution: 2.21 Å

EMDB-63550: Local refinement map of TBCE in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.59 Å

EMDB-63551: Local refinement map of TBCE and alpha-tubulin in the TBC-DEC-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.34 Å

EMDB-63552: Local refinement map of TBCC-RPD and Arl2 in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-63553: Local refinement map of TBCD and Arl2 in the TBC-DEC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.18 Å

EMDB-63554: Consensus map of the TBC-DC-Arl2-alpha-beta-tubulin complex with GDP-AlFx
Method: EM (single particle) / Resolution: 2.24 Å

EMDB-63555: Local refinement map of alpha-tubulin in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.37 Å

EMDB-63556: Local refinement map of TBCD and Arl2 in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.23 Å

EMDB-63557: Local refinement map of TBCC-RPD and Arl2 in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.34 Å

EMDB-63558: Local refinement map of TBCD AR3 domain in the TBC-DC-Arl2-alpha-beta-tubulin-GDP-AlFx complex
Method: EM (single particle) / Resolution: 2.94 Å

EMDB-63569: Consensus map of the TBC-DE-Arl2-beta-tubulin complex
Method: EM (single particle) / Resolution: 2.14 Å

EMDB-63570: Local refinement map of TBCE-LRRD in the TBC-DE-Arl2-beta-tubulin complex
Method: EM (single particle) / Resolution: 3.59 Å

EMDB-63572, PDB-9m1i:
Cryo-EM structure of the TBC-D-Arl2-beta-tubulin complex
Method: EM (single particle) / Resolution: 2.48 Å

EMDB-63573, PDB-9m1j:
Cryo-EM structure of the TBC-DE-Arl2-beta-tubulin complex
Method: EM (single particle) / Resolution: 2.14 Å

EMDB-63574, PDB-9m1k:
Cryo-EM structure of the TBC-DE-Arl2-beta-tubulin complex with GTP
Method: EM (single particle) / Resolution: 2.45 Å

EMDB-63575, PDB-9m1l:
Cryo-EM structure of the TBC-DE-Arl2-alpha-beta-tubulin complex with GTP
Method: EM (single particle) / Resolution: 2.55 Å

EMDB-63576, PDB-9m1m:
Cryo-EM structure of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx
Method: EM (single particle) / Resolution: 2.21 Å

EMDB-63577, PDB-9m1n:
Cryo-EM structure of the TBC-DC-Arl2-alpha-beta-tubulin complex with GDP-AlFx
Method: EM (single particle) / Resolution: 2.24 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-AF3:
ALUMINUM FLUORIDE

Source
  • homo sapiens (human)
  • sus scrofa (pig)
KeywordsCHAPERONE / complex

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