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Structure paper

TitleDeciphering Structural Traits for Thermal and Kinetic Stability across Protein Family Evolution through Ancestral Sequence Reconstruction
Journal, issue, pagesMol. Biol. Evol., Vol. 41, Year 2024
Publish dateJun 27, 2021 (structure data deposition date)
AuthorsCea, P.A. / Perez, M. / Herrera, S.M. / Munoz, S.M. / Fuentes-Ugarte, N. / Coche-Miranda, J. / Maturana, P. / Guixe, V. / Castro-Fernandez, V.
External linksMol. Biol. Evol. / Search PubMed
MethodsX-ray diffraction
Resolution2.3 - 3.2 Å
Structure data

PDB-7r8y:
Ancestral protein AncThEn of Phosphomethylpyrimidine kinases family
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-7r8z:
Ancestral protein AncEn of Phosphomethylpyrimidine kinases family
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HMH:
4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE

ChemComp-TAR:
D(-)-TARTARIC ACID

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsTRANSFERASE / ancestral protein reconstruction / 5-Phosphohydroxymethyl-2-methylpyrimidine kinase / Phosphomethylpyrimidine kinases family / enzyme evolution

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