Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Ligand binding modes of the bitter taste receptor T2R14 and T2R46.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 33, Issue 4, Page 691-700, Year 2026
Publish date	Apr 20, 2026
Authors	Qiuxiang Tan / Yu Yu / Xuteng Han / Kezhen Liu / Shuo Han / Mu Wang / Yuan Wei / Yingrong Zhu / Qiuru Chen / Limin Ma / Cuiying Yi / Xiaojing Chu / Beili Wu / Qiang Zhao /
PubMed Abstract	Bitter taste receptors (T2Rs) are considered attractive drug targets. However, the ligand recognition and selectivity of these receptors remain elusive, hampering their drug development. Here we ...Bitter taste receptors (T2Rs) are considered attractive drug targets. However, the ligand recognition and selectivity of these receptors remain elusive, hampering their drug development. Here we present seven structures of human T2R14 and T2R46 in apo or ligand-bound state. Combined with molecular docking and mutagenesis data, the structures reveal an extracellular ligand-binding site in T2R14 for most of its ligands, which is different from the intracellular binding site reported recently. In contrast, T2R46 exhibits a conserved binding pocket that accommodates various ligands with distinct interaction patterns. Furthermore, the second extracellular loop in T2R14 and T2R46 acts as a tethered agonist to potentially facilitate agonist response of these two receptors to the weak tastant agonists. These findings could accelerate drug discovery targeting T2Rs.
External links	Nat Struct Mol Biol / PubMed:42009775 / PubMed Central
Methods	EM (single particle)
Resolution	2.7 - 3.6 Å
Structure data	65511 9w0p EMDB-65511, PDB-9w0p: CryoEM structure of T2R14 in complex with chlorhexidine and heterotrimeric G protein complex Method: EM (single particle) / Resolution: 3.6 Å 65512 9w0q EMDB-65512, PDB-9w0q: Cryo-EM structure of T2R14 in complex with tangeretin and heterotrimeric G protein Method: EM (single particle) / Resolution: 3.2 Å 65513 9w0r EMDB-65513, PDB-9w0r: Cryo-EM structure of apo-T2R46 in complex with heterotrimeric G protein Method: EM (single particle) / Resolution: 2.7 Å 65514 9w0s EMDB-65514, PDB-9w0s: CryoEM structure of T2R46 in complex with Arteminisin and heterotrimeric G protein complex Method: EM (single particle) / Resolution: 3.3 Å 65515 9w0t EMDB-65515, PDB-9w0t: CryoEM structure of the T2R46 in complex with Denatomium benozate and heterotrimeric G protein complex Method: EM (single particle) / Resolution: 3.3 Å 65516 9w0u EMDB-65516, PDB-9w0u: CryoEM structure of the T2R46 in complex with tangeretin and heterotrimeric G protein complex Method: EM (single particle) / Resolution: 2.9 Å 65518 9w0w EMDB-65518, PDB-9w0w: CryoEM structure of the T2R46 in complex with strychine and heterotrimeric G protein complex Method: EM (single particle) / Resolution: 3.2 Å
Chemicals	ChemComp-XC9: 1-[6-[azanylidene-[[azanylidene-[[(4-chlorophenyl)amino]methyl]-$l^{4}-azanyl]methyl]-$l^{4}-azanyl]hexyl]-3-[~{N}-(4-chlorophenyl)carbamimidoyl]guanidine PDB-1eui: ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN PDB-1euw: ATOMIC RESOLUTION STRUCTURE OF E. COLI DUTPASE ChemComp-WK3: N-benzyl-2-(2,6-dimethylanilino)-N,N-diethyl-2-oxoethan-1-aminium ChemComp-SY9: STRYCHNINE
Source	homo sapiens (human) escherichia coli (E. coli)
Keywords	MEMBRANE PROTEIN / G protein-coupled receptor / Bitter taste receptor 14 / Bitter taste receptor 46 / ligand binding / signal transduction