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TitleAsymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus.
Journal, issue, pagesSci Adv, Vol. 11, Issue 50, Page eady4104, Year 2025
Publish dateDec 12, 2025
AuthorsSean M Braet / Varun Venkatakrishnan / Ranita Ramesh / Molly A Clawson / Tatiana N Laremore / Sek-Man Wong / Ganesh S Anand /
PubMed AbstractIcosahedral RNA viruses rely upon essential asymmetries for directional genome egress into the host cell. How these asymmetric egress points are built into the quaternary assembly of the virion is ...Icosahedral RNA viruses rely upon essential asymmetries for directional genome egress into the host cell. How these asymmetric egress points are built into the quaternary assembly of the virion is unknown. Here, we capture the structure and dynamics of a partially expanded virus disassembly intermediate, poised to release its spring-loaded genomic RNA. The structure shows highly localized density of RNA underneath surface fivefold axes on one-half of the viral particle. This polarity in RNA distribution is associated with a unique interchain isopeptide bond (glutamic acid-lysine), which flanks conserved high-affinity RNA binding sites. This singular isopeptide bond at the asymmetric egress site confers an essential "loaded-die" feature that is critical for steering genomic RNA egress into the host cell for virus propagation.
External linksSci Adv / PubMed:41385643 / PubMed Central
MethodsEM (single particle)
Resolution2.87 - 3.62 Å
Structure data

72428

9y2z

EMDB-72428, PDB-9y2z:
Icosahedral symmetric structure of an expansion intermediate of Turnip Crinkle Virus (Asymmetric Trimer Unit)
Method: EM (single particle) / Resolution: 2.87 Å

72430

9y31

EMDB-72430, PDB-9y31:
Symmetry relaxed asymmetric structure of an expansion intermediate of Turnip crinkle virus
Method: EM (single particle) / Resolution: 3.62 Å

Source
  • turnip crinkle virus
KeywordsVIRUS / Icosahedral non-enveloped ssRNA virus / Expansion intermediate / VIRAL PROTEIN

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