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TitleHuman Schlafen 14 Cleavage of Short Double-Stranded RNAs Underpins its Antiviral Activity.
Journal, issue, pagesAdv Sci (Weinh), Vol. 12, Issue 37, Page e01727, Year 2025
Publish dateJul 11, 2025
AuthorsMengyun Li / Dapeng Sun / Wei Hao / Hua Fu / Yueli Zhang / Zhijian Li / Bo Qin / Yumei Wang / Sheng Cui /
PubMed AbstractThe Schlafen (SLFN) genes are induced by interferons, underscoring their roles in the immune response and viral replication inhibition. SLFN14, a member of SLFN family, is associated with multiple ...The Schlafen (SLFN) genes are induced by interferons, underscoring their roles in the immune response and viral replication inhibition. SLFN14, a member of SLFN family, is associated with multiple human diseases; however, neither its functions nor its disease mechanisms are fully understood. Herein, human SLFN14 biochemically is characterized, demonstrating that it specifically cleaves RNAs containing short duplex regions, such as hairpin RNAs and tRNAs, but does not have ATPase or helicase activity. Cryogenic electron microscopy structures of SLFN14 apoenzyme (2.84 Å) and SLFN14-hairpin RNA complex (2.88 Å) are determined, revealing that SLFN14 assembles into a ring-like dimer and dimerization is mainly mediated by hydrophobic contacts. Two N-terminal RNase domains of SLFN14 are organized head-to-tail, forming an RNA-binding groove that can accommodate a 12-bp hairpin RNA. The hairpin RNA is recognized mainly through phosphate backbone interactions. Further, SLFN14 is shown to inhibits HIV-1 pseudovirus replication. The anti-HIV-1 activity of SLFN14 is via codon-usage-dependent translational inhibition and impairment of the programmed -1 ribosomal frameshifting, with an efficiency comparable to that of Shiftless. Using tRNA PCR arrays, SLFN14 and SLFN11 are found to decrease both nuclear-encoded and mitochondrial tRNAs in cells. Together, these results provide novel insights into the function of SLFN14 and its role in HIV-1 restriction.
External linksAdv Sci (Weinh) / PubMed:40642785 / PubMed Central
MethodsEM (single particle)
Resolution2.84 - 2.88 Å
Structure data

61748

9jr9

EMDB-61748, PDB-9jr9:
Electronic microscopy structure of human schlafen14-E211A dimer
Method: EM (single particle) / Resolution: 2.84 Å

64191

9uie

EMDB-64191, PDB-9uie:
Electronic microscopy structure of human schlafen14-E211A dimer in complex with dsRNA
Method: EM (single particle) / Resolution: 2.88 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / Dimer / RNase / RNA BINDING PROTEIN/RNA / complex / RNA BINDING PROTEIN-RNA complex

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