EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Multiple steps of dynein activation by Lis1 visualized by cryo-EM.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 8, Page 1434-1444, Year 2025
掲載日	2025年5月23日
著者	Agnieszka A Kendrick / Kendrick H V Nguyen / Wen Ma / Eva P Karasmanis / Rommie E Amaro / Samara L Reck-Peterson / Andres E Leschziner /
PubMed 要旨	Cytoplasmic dynein-1 (dynein) is an essential molecular motor controlled in part by autoinhibition. Lis1, a key dynein regulator mutated in the neurodevelopmental disease lissencephaly, plays a role ...Cytoplasmic dynein-1 (dynein) is an essential molecular motor controlled in part by autoinhibition. Lis1, a key dynein regulator mutated in the neurodevelopmental disease lissencephaly, plays a role in dynein activation. We recently identified a structure of partially autoinhibited dynein bound to Lis1, which suggests an intermediate state in dynein's activation pathway. However, other structural information is needed to fully understand how Lis1 activates dynein. Here, we used cryo-EM and yeast dynein and Lis1 incubated with ATP at different time points to reveal conformations that we propose represent additional intermediate states in dynein's activation pathway. We solved 16 high-resolution structures, including 7 distinct dynein and dynein-Lis1 structures from the same sample. Our data support a model in which Lis1 relieves dynein autoinhibition by increasing its basal ATP hydrolysis rate and promoting conformations compatible with complex assembly and motility. Together, this analysis advances our understanding of dynein activation and the contribution of Lis1 to this process.
リンク	Nat Struct Mol Biol / PubMed:40410592 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.1 Å
構造データ	46897 9di3 EMDB-46897, PDB-9di3: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 4.1 Å 46919 9diu EMDB-46919, PDB-9diu: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 4.1 Å 46935 9dj7 EMDB-46935, PDB-9dj7: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.8 Å 46938 9dju EMDB-46938, PDB-9dju: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 4.0 Å 46940 9djy EMDB-46940, PDB-9djy: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.6 Å 46941 9djz EMDB-46941, PDB-9djz: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.9 Å 46942 9dk0 EMDB-46942, PDB-9dk0: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.7 Å 46953 9dkd EMDB-46953, PDB-9dkd: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.3 Å 46954 9dke EMDB-46954, PDB-9dke: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.6 Å 46958 9dkh EMDB-46958, PDB-9dkh: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.9 Å 46959 9dkj EMDB-46959, PDB-9dkj: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 2.8 Å 46962 9dkm EMDB-46962, PDB-9dkm: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.4 Å 46972 9dkx EMDB-46972, PDB-9dkx: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.7 Å 46974 9dld EMDB-46974, PDB-9dld: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.2 Å 46975 9dle EMDB-46975, PDB-9dle: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.4 Å 47019 9dmw EMDB-47019, PDB-9dmw: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.7 Å 47026 9dn5 EMDB-47026, PDB-9dn5: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.3 Å 47032 9dn7 EMDB-47032, PDB-9dn7: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.25 Å 47033 9dnb EMDB-47033, PDB-9dnb: CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1. 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母)
キーワード	MOTOR PROTEIN / Enzyme / AAA protein / AAA+ protein