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TitleStructural insights into auxin influx mediated by the Arabidopsis AUX1.
Journal, issue, pagesCell, Vol. 188, Issue 15, Page 3960-3973.e15, Year 2025
Publish dateJul 24, 2025
AuthorsZhisen Yang / Hong Wei / Yulin Gan / Huihui Liu / Yang Cao / Huihui An / Xiuzheng Que / Yongxiang Gao / Lizhe Zhu / Shutang Tan / Xin Liu / Linfeng Sun /
PubMed AbstractAuxin is crucial in orchestrating diverse aspects of plant growth and development and modulating responses to environmental signals. The asymmetric spatiotemporal distribution of auxin generates ...Auxin is crucial in orchestrating diverse aspects of plant growth and development and modulating responses to environmental signals. The asymmetric spatiotemporal distribution of auxin generates local gradient patterns, which are regulated by both cellular auxin influx and efflux. The AUXIN1/LIKE-AUX1 (AUX1/LAX) family transporters have been identified as major auxin influx carriers. Here, we characterize the auxin uptake mediated by AUX1 from Arabidopsis thaliana. Using cryoelectron microscopy (cryo-EM), we determine its structure in three states: the auxin-unbound, the auxin-bound, and the competitive inhibitor, 3-chloro-4-hydroxyphenylacetic acid (CHPAA)-bound state. All structures adopt an inward-facing conformation. In the auxin-bound structure, indole-3-acetic acid (IAA) is coordinated to AUX1 primarily through hydrogen bonds with its carboxyl group. The functional roles of key residues in IAA binding are validated by in vitro and in planta analyses. CHPAA binds to the same site as IAA. These findings advance our understanding of auxin transport in plants.
External linksCell / PubMed:40378849
MethodsEM (single particle)
Resolution3.4 - 3.5 Å
Structure data

61397

9jdr

EMDB-61397, PDB-9jdr:
Structure of the auxin importer AUX1 in Arabidopsis thaliana in the apo state
Method: EM (single particle) / Resolution: 3.5 Å

61398

9jds

EMDB-61398, PDB-9jds:
Structure of the auxin importer AUX1 in Arabidopsis thaliana in the IAA-bound state
Method: EM (single particle) / Resolution: 3.4 Å

63585

9m2h

EMDB-63585, PDB-9m2h:
Structure of the auxin importer AUX1 in Arabidopsis thaliana in the CHPAA-bound state
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-IAC:
1H-INDOL-3-YLACETIC ACID / hormone*YM

ChemComp-HOH:
WATER

ChemComp-3C4:
(3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID

Source
  • arabidopsis thaliana (thale cress)
KeywordsMEMBRANE PROTEIN / Auxin / AUX1 / Importer

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