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- EMDB-63585: Structure of the auxin importer AUX1 in Arabidopsis thaliana in t... -

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Basic information

Entry
Database: EMDB / ID: EMD-63585
TitleStructure of the auxin importer AUX1 in Arabidopsis thaliana in the CHPAA-bound state
Map data
Sample
  • Complex: AUX1 protein in complex with CHPAA
    • Protein or peptide: Auxin transporter protein 1
  • Ligand: (3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID
KeywordsAuxin / importer / AUX1 / MEMBRANE PROTEIN
Function / homology
Function and homology information


root hair cell differentiation / root cap development / auxin binding / lateral root formation / auxin influx transmembrane transporter activity / positive gravitropism / auxin polar transport / establishment of planar polarity / auxin-activated signaling pathway / amino acid transmembrane transporter activity ...root hair cell differentiation / root cap development / auxin binding / lateral root formation / auxin influx transmembrane transporter activity / positive gravitropism / auxin polar transport / establishment of planar polarity / auxin-activated signaling pathway / amino acid transmembrane transporter activity / symporter activity / response to nematode / endosome / cell surface / Golgi apparatus / plasma membrane
Similarity search - Function
Amino acid transporter, transmembrane domain / Transmembrane amino acid transporter protein
Similarity search - Domain/homology
Auxin transporter protein 1
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsSun L / Liu X / Wei H / Yang Z
Funding support China, 3 items
OrganizationGrant numberCountry
National Science Foundation (NSF, China)32322041 China
National Science Foundation (NSF, China)32321001 China
National Science Foundation (NSF, China)31900885 China
CitationJournal: To Be Published
Title: Structure of the auxin importer AUX1 in Arabidopsis thaliana in the CHPAA-bound state
Authors: Sun L / Liu X / Wei H / Yang Z
History
DepositionFeb 27, 2025-
Header (metadata) releaseMay 28, 2025-
Map releaseMay 28, 2025-
UpdateJun 11, 2025-
Current statusJun 11, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_63585.png

Downloads & links

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Map

FileDownload / File: emd_63585.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 256 pix.
= 281.6 Å		1.1 Å/pix.
x 256 pix.
= 281.6 Å		1.1 Å/pix.
x 256 pix.
= 281.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.4
Minimum - Maximum-1.8170977 - 2.6219301
Average (Standard dev.)0.00012901684 (±0.04764482)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 281.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_63585_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_63585_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : AUX1 protein in complex with CHPAA

EntireName: AUX1 protein in complex with CHPAA
Components
  • Complex: AUX1 protein in complex with CHPAA
    • Protein or peptide: Auxin transporter protein 1
  • Ligand: (3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID

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Supramolecule #1: AUX1 protein in complex with CHPAA

SupramoleculeName: AUX1 protein in complex with CHPAA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Arabidopsis thaliana (thale cress)

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Macromolecule #1: Auxin transporter protein 1

MacromoleculeName: Auxin transporter protein 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 54.10552 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MSEGVEAIVA NDNGTDQVNG NRTGKDNEEH DGSTGSNLSN FLWHGGSVWD AWFSCASNQV AQVLLTLPYS FSQLGMLSGI VLQIFYGLL GSWTAYLISV LYVEYRARKE KEGKSFKNHV IQWFEVLDGL LGSYWKALGL AFNCTFLLFG SVIQLIACAS N IYYINDHL ...String:
MSEGVEAIVA NDNGTDQVNG NRTGKDNEEH DGSTGSNLSN FLWHGGSVWD AWFSCASNQV AQVLLTLPYS FSQLGMLSGI VLQIFYGLL GSWTAYLISV LYVEYRARKE KEGKSFKNHV IQWFEVLDGL LGSYWKALGL AFNCTFLLFG SVIQLIACAS N IYYINDHL DKRTWTYIFG ACCATTVFIP SFHNYRIWSF LGLGMTTYTA WYLAIASIIH GQAEGVKHSG PTKLVLYFTG AT NILYTFG GHAVTVEIMH AMWKPQKFKY IYLMATLYVF TLTIPSAAAV YWAFGDALLD HSNAFSLMPK NAWRDAAVIL MLI HQFITF GFACTPLYFV WEKVIGMHDT KSICLRALAR LPVVIPIWFL AIIFPFFGPI NSAVGALLVS FTVYIIPSLA HMLT YRSAS ARQNAAEKPP FFMPSWTAMY VLNAFVVVWV LIVGFGFGGW ASVTNFVRQV DTFGLFAKCY QCKPAAAAAH APVSA LHHR L

UniProtKB: Auxin transporter protein 1

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Macromolecule #2: (3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID

MacromoleculeName: (3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID / type: ligand / ID: 2 / Number of copies: 1 / Formula: 3C4
Molecular weightTheoretical: 186.592 Da
Chemical component information

ChemComp-3C4:
(3-CHLORO-4-HYDROXYPHENYL)ACETIC ACID

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 239360
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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