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-Structure paper
タイトル | Naturally ornate RNA-only complexes revealed by cryo-EM. |
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ジャーナル・号・ページ | Nature, Year 2025 |
掲載日 | 2025年5月6日 |
![]() | Rachael C Kretsch / Yuan Wu / Svetlana A Shabalina / Hyunbin Lee / Grace Nye / Eugene V Koonin / Alex Gao / Wah Chiu / Rhiju Das / ![]() |
PubMed 要旨 | The structures of natural RNAs remain poorly characterized and may hold numerous surprises. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron ...The structures of natural RNAs remain poorly characterized and may hold numerous surprises. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron microscopy (cryo-EM). GOLLD (Giant, Ornate, Lake- and Lactobacillales-Derived), ROOL (Rumen-Originating, Ornate, Large) and OLE (Ornate Large Extremophilic) RNAs form homo-oligomeric complexes whose stoichiometries are retained at lower concentrations than measured in cells. OLE RNA forms a dimeric complex with long co-axial pipes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome, each empty except for a disordered loop. Extensive intramolecular and intermolecular A-minor interactions, kissing loops, an unusual A-A helix and other interactions stabilize the three complexes. Sequence covariation analysis of these large RNAs reveals evolutionary conservation of intermolecular interactions, supporting the biological importance of large, ornate RNA quaternary structures that can assemble without any involvement of proteins. |
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手法 | EM (単粒子) |
解像度 | 2.9 - 3.7 Å |
構造データ | EMDB-48162, PDB-9ely: EMDB-48163, PDB-9mcw: EMDB-48179, PDB-9mds: EMDB-48214, PDB-9mee: ![]() EMDB-70247: Focused refinement of ' domain of a subunit of GOLLD RNA nanocage. ![]() EMDB-70248: Focused refinement of 5' domain of a single subunit of GOLLD RNA nanocage. ![]() EMDB-70249: D7 reconstruction of GOLLD nanocage ![]() EMDB-70250: Focused refinement of one subunit or ROOL RNA nanocage ![]() EMDB-70251: D4 reconstruction of ROOL RNA nanocage |
由来 |
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![]() | RNA / raiA / ncRNA / OLE / ROOL / nanocage / GOLLD |