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Structure paper

TitleUnraveling the molecular mechanism of polysaccharide lyases for efficient alginate degradation.
Journal, issue, pagesNat Commun, Vol. 16, Page 2670-2670, Year 2025
Publish dateApr 29, 2020 (structure data deposition date)
AuthorsRivas-Fernandez, J.P. / Vuillemin, M. / Pilgaard, B. / Klau, L.J. / Fredslund, F. / Lund-Hanssen, C. / Welner, D.H. / Meyer, A.S. / Morth, J.P. / Meilleur, F. ...Rivas-Fernandez, J.P. / Vuillemin, M. / Pilgaard, B. / Klau, L.J. / Fredslund, F. / Lund-Hanssen, C. / Welner, D.H. / Meyer, A.S. / Morth, J.P. / Meilleur, F. / Aachmann, F.L. / Rovira, C. / Wilkens, C.
External linksNat Commun / PubMed:40102416
MethodsX-ray diffraction
Resolution0.82 - 1.9 Å
Structure data

PDB-6ywf:
Crystal structure of Paradendryphiella salina PL7A alginate lyase
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7ncz:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with hexa-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-7nl3:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7nm6:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with di-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-7npp:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with penta-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-7ny3:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tetra-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-7o6h:
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tri-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-7oof:
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with tri-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7ory:
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with penta-mannuronic acid products
Method: X-RAY DIFFRACTION / Resolution: 1.46 Å

PDB-7p25:
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with hexa-mannuronic acid products
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-7p90:
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with tetra-mannuronic acid products
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

PDB-7pbf:
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with di-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.54 Å

PDB-8bjo:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with hexa-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.51 Å

PDB-8bxz:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with penta-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-8c0m:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-8c3x:
Crystal structure of Paradendryphiella salina PL7C alginate lyase
Method: X-RAY DIFFRACTION / Resolution: 0.82 Å

PDB-8p6o:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with di-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.05 Å

PDB-8pc3:
Crystal structure of Paradendryphiella salina PL7C alginate lyase in complex with pentamannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-8pc8:
Crystal structure of Paradendryphiella salina PL7C alginate lyase soaked with hexamannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.24 Å

PDB-8pcx:
Crystal structure of Paradendryphiella salina PL7C alginate lyase soaked with tetramannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.14 Å

PDB-8pdt:
Crystal structure of Paradendryphiella salina PL7C alginate lyase soaked with dimannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.09 Å

PDB-8ped:
Crystal structure of Paradendryphiella salina PL7C alginate lyase soaked with trimannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-8qiz:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124N soaked with with penta-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.13 Å

PDB-8qli:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124 soaked with with tetra-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.12 Å

PDB-8qmj:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124 soaked with hexa-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 0.98 Å

PDB-8r43:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H110N in complex with tri-mannuronic acid
Method: X-RAY DIFFRACTION / Resolution: 0.87 Å

PDB-8rbi:
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124
Method: X-RAY DIFFRACTION / Resolution: 0.98 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-BTB:
2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-MAV:
alpha-D-mannopyranuronic acid

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • paradendryphiella salina (fungus)
KeywordsLYASE / Alginate lyase / fungal / polysaccharide lyase family 7 / PL7 / complex / beta jelly roll / mutant / beta-jelly roll / beta-jeely roll / mannuronate / beta jelly-roll / Alginate lyase. beta-jelly roll

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