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TitleStructure-Guided Engineering of a Versatile Urethanase Improves Its Polyurethane Depolymerization Activity.
Journal, issue, pagesAdv Sci, Vol. 12, Page e2416019-e2416019, Year 2025
Publish dateSep 16, 2023 (structure data deposition date)
AuthorsLi, Z. / Han, X. / Cong, L. / Singh, P. / Paiva, P. / Branson, Y. / Li, W. / Chen, Y. / Jaradat, D.M.M. / Lennartz, F. ...Li, Z. / Han, X. / Cong, L. / Singh, P. / Paiva, P. / Branson, Y. / Li, W. / Chen, Y. / Jaradat, D.M.M. / Lennartz, F. / Bayer, T. / Schmidt, L. / Garscha, U. / You, S. / Fernandes, P.A. / Ramos, M.J. / Bornscheuer, U.T. / Weber, G. / Wei, R. / Liu, W.
External linksAdv Sci / PubMed:39921299
MethodsX-ray diffraction
Resolution2.16 - 2.59 Å
Structure data

PDB-8wdw:
Crystal structure of a novel PU plastic degradation urethanase UMG-SP2 from uncultured bacterium
Method: X-RAY DIFFRACTION / Resolution: 2.16 Å

PDB-8xtc:
Crystal structure of a novel PU plastic degradation urethanase UMG-SP2 mutant from uncultured bacterium in complex with ligand
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-9fzw:
Structure of Urethanase UMG-SP2
Method: X-RAY DIFFRACTION / Resolution: 2.59 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-PMS:
phenylmethanesulfonic acid

ChemComp-HOH:
WATER

PDB-1d5h:
Rnase s(f8a). mutant ribonucleasE S.

Source
  • uncultured bacterium (environmental samples)
  • metagenome (others)
KeywordsHYDROLASE / PU plastic / degradation / catalysis / urethanase / PU / mutant / urethanases / polyurethane / plastic degradation / polymer degradation / enzyme engineering

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