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Structure paper

TitleKorB switching from DNA-sliding clamp to repressor mediates long-range gene silencing in a multi-drug resistance plasmid.
Journal, issue, pagesNat Microbiol, Vol. 10, Page 448-467, Year 2025
Publish dateAug 22, 2023 (structure data deposition date)
AuthorsMcLean, T.C. / Balaguer-Perez, F. / Chandanani, J. / Thomas, C.M. / Aicart-Ramos, C. / Burick, S. / Olinares, P.D.B. / Gobbato, G. / Mundy, J.E.A. / Chait, B.T. ...McLean, T.C. / Balaguer-Perez, F. / Chandanani, J. / Thomas, C.M. / Aicart-Ramos, C. / Burick, S. / Olinares, P.D.B. / Gobbato, G. / Mundy, J.E.A. / Chait, B.T. / Lawson, D.M. / Darst, S.A. / Campbell, E.A. / Moreno-Herrero, F. / Le, T.B.K.
External linksNat Microbiol / PubMed:39849085
MethodsX-ray diffraction
Resolution2.3 - 2.7 Å
Structure data

PDB-8qa8:
Crystal structure of the C-terminally truncated transcriptional repressor protein KorB from the RK2 plasmid complexed with CTP-gamma-S
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-8qa9:
Crystal structure of the RK2 plasmid encoded co-complex of the C-terminally truncated transcriptional repressor protein KorB complexed with the partner repressor protein KorA bound to OA-DNA
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-CTP:
CYTIDINE-5'-TRIPHOSPHATE

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • escherichia coli (E. coli)
KeywordsDNA BINDING PROTEIN / multi-drug resistance / ParABS / clamp / CTP switch / DNA segregation / gene expression regulation

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