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Structure paper

TitleStructure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide.
Journal, issue, pagesOpen Biology, Vol. 15, Page 240310-240310, Year 2025
Publish dateJul 24, 2024 (structure data deposition date)
AuthorsLe Bas, A. / Clarke, B.R. / Teelucksingh, T. / Lee, M. / El Omari, K. / Giltrap, A.M. / McMahon, S.A. / Liu, H. / Beale, J.H. / Mykhaylyk, V. ...Le Bas, A. / Clarke, B.R. / Teelucksingh, T. / Lee, M. / El Omari, K. / Giltrap, A.M. / McMahon, S.A. / Liu, H. / Beale, J.H. / Mykhaylyk, V. / Duman, R. / Paterson, N.G. / Ward, P.N. / Harrison, P.J. / Weckener, M. / Pardon, E. / Steyaert, J. / Quigley, A. / Davis, B.G. / Wagner, A. / Whitfield, C. / Naismith, J.H.
External linksOpen Biology / PubMed:39772807
MethodsX-ray diffraction
Resolution2.31 - 2.8 Å
Structure data

PDB-9g95:
Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 2.7552 A
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9g97:
Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 0.9688 A
Method: X-RAY DIFFRACTION / Resolution: 2.31 Å

PDB-9g9m:
Lipid III flippase WzxE with NB10 and NB7 nanobodies in outward-facing conformation - crystal 1
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-9g9n:
Lipid III flippase WzxE with NB10 and NB7 nanobodies in inward-facing conformation - crystal 1
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9g9o:
Lipid III flippase WzxE with NB10 and NB7 nanobodies in outward-facing conformation - crystal 2
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

PDB-9g9p:
Lipid III flippase WzxE with NB10 and NB7 nanobodies in inward-facing conformation - crystal 2
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-MPG:
[(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate

ChemComp-OCT:
N-OCTANE

ChemComp-SO4:
SULFATE ION

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-LOP:
(1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE / phospholipid*YM

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

Source
  • escherichia coli (E. coli)
  • lama glama (llama)
KeywordsTRANSPORT PROTEIN / membrane protein / flippase / cell wall / enterobacterial common antigen / lipid III

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