EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanisms underlying modulation of human GlyRα3 by Zn and pH.
ジャーナル・号・ページ	Sci Adv, Vol. 10, Issue 51, Page eadr5920, Year 2024
掲載日	2024年12月20日
著者	Kayla Kindig / Eric Gibbs / David Seiferth / Philip C Biggin / Sudha Chakrapani /
PubMed 要旨	Glycine receptors (GlyRs) regulate motor control and pain processing in the central nervous system through inhibitory synaptic signaling. The subtype GlyRα3 expressed in nociceptive sensory neurons ...Glycine receptors (GlyRs) regulate motor control and pain processing in the central nervous system through inhibitory synaptic signaling. The subtype GlyRα3 expressed in nociceptive sensory neurons of the spinal dorsal horn is a key regulator of physiological pain perception. Disruption of spinal glycinergic inhibition is associated with chronic inflammatory pain states, making GlyRα3 an attractive target for pain treatment. GlyRα3 activity is modulated by numerous endogenous and exogenous ligands that consequently affect pain sensitization. To understand the mechanism of two such endogenous modulators, Zn and protons, we have used cryo-electron microscopy to determine structures of full-length human GlyRα3 in various functional states. Whereas acidic pH reduces peak glycine response, Zn displays biphasic modulation in a concentration-dependent manner. Our findings reveal the effector sites and also capture intermediate conformations in the gating cycle. Combined with molecular dynamics simulations and electrophysiology, this work provides important insights into GlyRα3 activation and regulation.
リンク	Sci Adv / PubMed:39693447 / PubMed Central
手法	EM (単粒子)
解像度	2.19 - 3.07 Å
構造データ	44899 9bu2 EMDB-44899, PDB-9bu2: Homomeric alpha3 glycine receptor in the presence of 1 mM glycine at pH 6.4 in a desensitized state 手法: EM (単粒子) / 解像度: 2.87 Å 44900 9bu3 EMDB-44900, PDB-9bu3: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine in a desensitized state 手法: EM (単粒子) / 解像度: 2.8 Å 44933 9bvh EMDB-44933, PDB-9bvh: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine in an apo state. 手法: EM (単粒子) / 解像度: 2.58 Å 44934 9bvj EMDB-44934, PDB-9bvj: Homomeric alpha3 glycine receptor in the presence of 1 mM glycine in an desensitized state. 手法: EM (単粒子) / 解像度: 2.8 Å 44963 9bwb EMDB-44963, PDB-9bwb: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in a desensitized state 手法: EM (単粒子) / 解像度: 2.21 Å 44964 9bwc EMDB-44964, PDB-9bwc: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in an apo state 手法: EM (単粒子) / 解像度: 2.19 Å 44970 9bwe EMDB-44970, PDB-9bwe: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in an intermediate state 手法: EM (単粒子) / 解像度: 3.07 Å 44974 9bwg EMDB-44974, PDB-9bwg: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine and 0.1 mM zinc in a desensitized state 手法: EM (単粒子) / 解像度: 2.59 Å 44976 9bwj EMDB-44976, PDB-9bwj: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine and 0.1 mM zinc in an apo state 手法: EM (単粒子) / 解像度: 2.21 Å 45073 9bzp EMDB-45073, PDB-9bzp: Homomeric alpha3 glycine receptor in the presence of 0.1 millimolar glycine and 1 micromolar zinc in a desensitized state 手法: EM (単粒子) / 解像度: 2.88 Å
化合物	ChemComp-GLY: GLYCINE ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate ChemComp-PX4: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DMPC, リン脂質YM ChemComp-HOH: WATER ChemComp-CLR: CHOLESTEROL ChemComp-ZN*: Unknown entry
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / Glycine / Ion Channel / Ligand-Gated / Pentameric