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TitleStructural and Functional Characterization of an Amidase Targeting a Polyurethane for Sustainable Recycling.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 64, Page e202419535-e202419535, Year 2025
Publish dateJun 27, 2024 (structure data deposition date)
AuthorsRotilio, L. / Bayer, T. / Meinert, H. / Teixeira, L.M.C. / Johansen, M.B. / Sommerfeldt, A. / Petersen, A.R. / Sandahl, A. / Keller, M.B. / Holck, J. ...Rotilio, L. / Bayer, T. / Meinert, H. / Teixeira, L.M.C. / Johansen, M.B. / Sommerfeldt, A. / Petersen, A.R. / Sandahl, A. / Keller, M.B. / Holck, J. / Paiva, P. / Otzen, D.E. / Bornscheuer, U.T. / Wei, R. / Fernandes, P.A. / Ramos, M.J. / Westh, P. / Morth, J.P.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:39611359
MethodsX-ray diffraction
Resolution2 - 2.32 Å
Structure data

PDB-9fvf:
UMG-SP3 amidase from uncultured bacterium
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

PDB-9fw1:
UMG-SP3 amidase from uncultured bacterium in complex with PMSF
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

PDB-9fz1:
UMG-SP3 amidase from uncultured bacterium in complex with 4,4'-MDA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-PG6:
1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-PMS:
phenylmethanesulfonic acid

ChemComp-ACT:
ACETATE ION

PDB-1ihk:
CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 9 (FGF9)

Source
  • uncultured bacterium (environmental samples)
KeywordsHYDROLASE / amidase / urethane degradation / plastic recycling / PMSF / urethane degrading / urethane bond / 4 / 4'-MDA

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