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TitleMechanism for Vipp1 spiral formation, ring biogenesis, and membrane repair.
Journal, issue, pagesNat Struct Mol Biol, Vol. 32, Issue 3, Page 571-584, Year 2025
Publish dateNov 11, 2024
AuthorsSouvik Naskar / Andrea Merino / Javier Espadas / Jayanti Singh / Aurelien Roux / Adai Colom / Harry H Low /
PubMed AbstractThe ESCRT-III-like protein Vipp1 couples filament polymerization with membrane remodeling. It assembles planar sheets as well as 3D rings and helical polymers, all implicated in mitigating plastid- ...The ESCRT-III-like protein Vipp1 couples filament polymerization with membrane remodeling. It assembles planar sheets as well as 3D rings and helical polymers, all implicated in mitigating plastid-associated membrane stress. The architecture of Vipp1 planar sheets and helical polymers remains unknown, as do the geometric changes required to transition between polymeric forms. Here we show how cyanobacterial Vipp1 assembles into morphologically-related sheets and spirals on membranes in vitro. The spirals converge to form a central ring similar to those described in membrane budding. Cryo-EM structures of helical filaments reveal a close geometric relationship between Vipp1 helical and planar lattices. Moreover, the helical structures reveal how filaments twist-a process required for Vipp1, and likely other ESCRT-III filaments, to transition between planar and 3D architectures. Overall, our results provide a molecular model for Vipp1 ring biogenesis and a mechanism for Vipp1 membrane stabilization and repair, with implications for other ESCRT-III systems.
External linksNat Struct Mol Biol / PubMed:39528797 / PubMed Central
MethodsEM (helical sym.)
Resolution3.67 - 3.78 Å
Structure data

18318

8qbr

EMDB-18318, PDB-8qbr:
Cryo-EM structure of Vipp1 helical filament with lattice 1 (Vipp1_L1)
Method: EM (helical sym.) / Resolution: 3.74 Å

18319

8qbs

EMDB-18319, PDB-8qbs:
Cryo-EM structure of Vipp1-F197K/L200K helical filament with lattice 1 (Vipp1-F197K/L200K_L1)
Method: EM (helical sym.) / Resolution: 3.67 Å

18321

8qbv

EMDB-18321, PDB-8qbv:
Cryo-EM structure of Vipp1-deltaH6_aa1-219 helical filament with lattice 2 (Vipp1-deltaH6_L2)
Method: EM (helical sym.) / Resolution: 3.78 Å

18322

8qbw

EMDB-18322, PDB-8qbw:
Cryo-EM structure of Vipp1-deltaH6_aa1-219 helical filament with lattice 3 (Vipp1-deltaH6_L3)
Method: EM (helical sym.) / Resolution: 3.67 Å

Source
  • nostoc punctiforme (bacteria)
KeywordsLIPID BINDING PROTEIN / Vipp1/IM30/ESCRT-III / Membrane remodeling / Cryoelectron microscopy / Helical filament structure

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