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TitleStructure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii.
Journal, issue, pagesEMBO J, Vol. 44, Issue 4, Page 975-990, Year 2025
Publish dateOct 16, 2024
AuthorsYichen Lu / Ke Liang / Xiechao Zhan /
PubMed AbstractPre-mRNA splicing, a fundamental step in eukaryotic gene expression, is executed by the spliceosomes. While there is extensive knowledge of the composition and structure of spliceosomes in yeasts and ...Pre-mRNA splicing, a fundamental step in eukaryotic gene expression, is executed by the spliceosomes. While there is extensive knowledge of the composition and structure of spliceosomes in yeasts and humans, the structural diversity of spliceosomes in non-canonical organisms remains unclear. Here, we present a cryo-EM structure of a step II catalytically activated spliceosome (C complex) derived from the unicellular green alga Chlamydomonas reinhardtii at 2.6 Å resolution. This Chlamydomonas C complex comprises 29 proteins and four RNA elements, creating a dynamic assembly that shares a similar overall architecture with yeast and human counterparts but also has unique features of its own. Distinctive structural characteristics include variations in protein compositions as well as some noteworthy RNA features. The splicing factor Prp17, with four fragments and a WD40 domain, is engaged in intricate interactions with multiple protein and RNA components. The structural elucidation of Chlamydomonas C complex provides insights into the molecular mechanism of RNA splicing in plants and understanding splicing evolution in eukaryotes.
External linksEMBO J / PubMed:39415054 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.6 Å
Structure data

38362

8xi2

EMDB-38362: Cryo-EM map of the Chlamydomonas C* complex from Cdc5L-Flag dataset
PDB-8xi2: Cryo-EM structure of the Chlamydomonas C* complex
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-38363: Local EM map of Prp19 subcomplex of Chlamydomonas C* complex from Cdc5L-Flag dataset
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-38364: Local EM map of N-ter of Prp17 of Chlamydomonas C* complex from Cdc5L-Flag dataset
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-38365: Local EM map of U5 snRNP of Chlamydomonas C* complex from Cdc5L-Flag dataset
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-38366: Overall EM map of the Chlamydomonas C* complex from Prp19-Flag dataset
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-38367: Local EM map of Prp19 subcomplex of Chlamydomonas C* complex from Prp19-Flag dataset
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-38368: Local EM map of N-ter of Prp17 of Chlamydomonas C* from Prp19-Flag dataset
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
KeywordsSPLICING / Chlamydomonas spliceosome / C* complex / Cdc5L / RNA splicing

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