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- EMDB-38362: Cryo-EM map of the Chlamydomonas C* complex from Cdc5L-Flag dataset -

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Basic information

Entry
Database: EMDB / ID: EMD-38362
TitleCryo-EM map of the Chlamydomonas C* complex from Cdc5L-Flag dataset
Map data
Sample
  • Complex: Chlamydomonas C* complex
    • Protein or peptide: x 26 types
    • RNA: x 4 types
  • RNA: x 1 types
  • Ligand: x 3 types
KeywordsChlamydomonas spliceosome / C* complex / Cdc5L / RNA splicing / SPLICING
Function / homology
Function and homology information


post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / U12-type spliceosomal complex / Prp19 complex / pICln-Sm protein complex / snRNP binding / U2-type catalytic step 1 spliceosome / pre-mRNA binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex ...post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / U12-type spliceosomal complex / Prp19 complex / pICln-Sm protein complex / snRNP binding / U2-type catalytic step 1 spliceosome / pre-mRNA binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / mRNA cis splicing, via spliceosome / commitment complex / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / cyclosporin A binding / spliceosomal complex assembly / protein K63-linked ubiquitination / spliceosomal tri-snRNP complex assembly / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / chloroplast / peptidylprolyl isomerase / helicase activity / peptidyl-prolyl cis-trans isomerase activity / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / mRNA processing / ubiquitin-protein transferase activity / metallopeptidase activity / ubiquitin protein ligase activity / protein folding / DNA repair / GTPase activity / mRNA binding / GTP binding / DNA binding / RNA binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Torus domain / Torus domain / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / mRNA splicing factor SYF2 / SYF2 splicing factor ...Torus domain / Torus domain / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / mRNA splicing factor SYF2 / SYF2 splicing factor / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / : / : / Myb-like domain profile. / Pre-mRNA-processing factor 17 / HAT (Half-A-TPR) repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / : / STL11, N-terminal / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / DNA2/NAM7 helicase, helicase domain / Cwf15/Cwc15 cell cycle control protein / AAA domain / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / : / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / : / Myb-like DNA-binding domain / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / DNA2/NAM7-like helicase / Small nuclear ribonucleoprotein D1 / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / U-box domain profile. / Modified RING finger domain / Zinc finger, CCCH-type superfamily / U-box domain / zinc finger / Pre-mRNA-splicing factor Syf1-like / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Small nuclear ribonucleoprotein Sm D3 / : / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Myb-type HTH DNA-binding domain profile. / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm7/SmG / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / : / Sm domain profile. / LSM domain superfamily / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site / Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. / EF-G domain III/V-like / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / PROCT domain / Prp8 RNase domain IV, fingers region
Similarity search - Domain/homology
Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor SPF27 / Small nuclear ribonucleoprotein Sm D1 / Sm domain-containing protein / Crooked neck protein / Uncharacterized protein / G10 protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein ...Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor SPF27 / Small nuclear ribonucleoprotein Sm D1 / Sm domain-containing protein / Crooked neck protein / Uncharacterized protein / G10 protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / MI domain-containing protein / Intron-binding protein aquarius / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / MPN domain-containing protein / peptidylprolyl isomerase / SKI-interacting protein SKIP SNW domain-containing protein / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein E / Sm protein F / Elongation factor Tu, chloroplastic / Peptidyl-prolyl cis-trans isomerase
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsLu Y / Zhan X
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Structure of a Step II catalytically activated spliceosome form Chlamydomonas reinhardtii
Authors: Lu Y / Zhan X
History
DepositionDec 19, 2023-
Header (metadata) releaseOct 9, 2024-
Map releaseOct 9, 2024-
UpdateOct 9, 2024-
Current statusOct 9, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38362.png

Downloads & links

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Map

FileDownload / File: emd_38362.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.92 Å/pix.
x 440 pix.
= 404.8 Å		0.92 Å/pix.
x 440 pix.
= 404.8 Å		0.92 Å/pix.
x 440 pix.
= 404.8 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.92 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-1.5648737 - 2.5933805
Average (Standard dev.)0.0017652584 (±0.03768861)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 404.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38362_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38362_half_map_2.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Sample components

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Entire : Chlamydomonas C* complex

EntireName: Chlamydomonas C* complex
Components
  • Complex: Chlamydomonas C* complex
    • Protein or peptide: MPN domain-containing protein
    • RNA: U5 snRNA
    • Protein or peptide: Elongation factor Tu, chloroplastic
    • Protein or peptide: U5-40K
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Sm protein F
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Sm domain-containing protein
    • Protein or peptide: Syf1
    • Protein or peptide: Crooked neck protein
    • Protein or peptide: Cwf15/Cwc15 cell cycle control protein
    • Protein or peptide: PPIase cyclophilin-type domain-containing protein
    • Protein or peptide: PLRG1
    • Protein or peptide: Rbm22
    • Protein or peptide: G10 protein
    • Protein or peptide: SKI-interacting protein SKIP SNW domain-containing protein
    • RNA: U2 snRNA
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase
    • Protein or peptide: Prp17
    • Protein or peptide: Cdc5L
    • Protein or peptide: Pre-mRNA-splicing factor SPF27
    • Protein or peptide: CWF21 domain-containing protein
    • Protein or peptide: MI domain-containing protein
    • Protein or peptide: Intron-binding protein aquarius
    • RNA: RNA (5'-R(P*CP*CP*GP*AP*AP*CP*G)-3')
    • RNA: RNA (173-mer)
  • RNA: U6 snRNA
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Chlamydomonas C* complex

SupramoleculeName: Chlamydomonas C* complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4, #6-#31
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: MPN domain-containing protein

MacromoleculeName: MPN domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 278.543438 KDa
SequenceString: MEGGGPPPPP GGFRPPMPPG GFPMGGMPPG MRPPMVAPHQ TRYFNQMGGM APVGGIPPPP MGQAPGGAPR PAGPPPVPVL SEELQQKLL EEKAKKWAQL NAKRYGDKRK FGFVEAPKED MPPEHVRKII RDHGDMSSRK FRHDKRVYLG ALKFVPHAVF K LLENMPMP ...String:
MEGGGPPPPP GGFRPPMPPG GFPMGGMPPG MRPPMVAPHQ TRYFNQMGGM APVGGIPPPP MGQAPGGAPR PAGPPPVPVL SEELQQKLL EEKAKKWAQL NAKRYGDKRK FGFVEAPKED MPPEHVRKII RDHGDMSSRK FRHDKRVYLG ALKFVPHAVF K LLENMPMP WEQVRHVKVI YHITGAITFV NEIPWVIEPV YLAQWGSMWI MMRREKRDRR HFKRMRFPPF DDEEPPLDYA DN LLDVEPL EAIEMELDEE EDAAVYEWLY DHQPLKYTKF VNGPSYKRWK LPLPVMAALY RLSGQLLTDF PDRNYFYLFE PQA FVTAKS LNMCIPGGPK FEPLFRDMDT RDEDWNEFND INKLIIRSPI RTEYKVAFPY LYNNRPRKVR LSVYHHPMSL YIKT EDPDL PAYYYDPLIH PIAAYRSAAG GRRAKIEEEV GGDDDEWALP SGVEPLLGES PLYTDNTASG IALLWAPPPF NRRSG HTRR AFDVPLVNCW FQEHCPPTYP VKVRVSYQKL LKNYVLNLLH ARPPKSVKKK YLLRALKATK FFQSTELDWV EVGLQV CRQ GYNMLNLLIH RKNLNYLHLD YNFNLKPVKT LTTKERKKSR FGNAFHLCRE ILRLTKLVVD ANVQFRLGNV DAYQLAD GL QYIFAHVGQL TGMYRYKYRL MRQIRMCKDL KHLIYYRFNT GPVGKGPGVG FWAPMWRVWL FFLRGIVPLL ERWLGNLL A RQFEGRQSKG IAKTVTKQRI ESHFDLELRA AVMHDILDMM PEGVKQNKAR TILQHLSEAW RCWKANIPWK VPGLPAPVE NMILRYVKQK ADWWTNVAHY NRERIRRGAT VDKTVCRKNL GRLTRLYLKA EQERQHNYLK DGPYVTPEEA VAIYTTTVHW LESRKYSCI PFPPLNYKHD TKLLILALER LKEQYVVAVR LNSSQREELG LVEQAYDNPH EALQRIKRHL LTQRSFKEVA I EFMDLYSH LIPVYEIEPL EKITDCYLDQ YLWFEADNRH LFPCWVKPAD SEPPPLLVYK WCHGINNVTN VWDTNAGECV VM MQSEFEK MYDKIDLTLM NRLLRLIVDH NIADYITAKN NVVIAYKDMS HTNSYGLIRG LQFASFIIQY YGLILDLLLL GLT RATEIA GPPQCPNEFL TYRDVKTETR HPIRLYSRYL NKVHIMFRFS AEEAKDLIQR YLTEHPDPNN ENIVGYNNKK CWPR DARMR LMKHDVNLGR AVFWDMKNRL PRSLTTFEWD NSFVSVYSRD NPNLLFSMLG FEVRILPKIR MAAESFANKD GVWAL QNVH TKERTAQAFL RVDDEGLKSF ENRVRQVLMS SGSTTFTKIA NKWNTALIGL MTYYREAVLH TQELLDLLVK CENKIQ TRI KIGLNSKMPS RFPPVVFYSP KEVGGLGMLS MGHILIPQSD LRYSQQTDLG VTHFRAGMSH EEDQLIPNLY RYIQPWE SE FMDSERVWSE YALKKEEAKA QNRRLTLDDL DDSWDRGIPR INTLFQKDRH TLAYDRGWRV RQEMKMFQIT KMNPFWWT H QKHDGKLWNL NNYRTDVIQA LGGVEGILEH TLFKGTYFPT WEGLFWEKAS GFEESMKYKK LTNAQRSGLN QIPNRRFTL WWSPTINRAN VYVGFQVQLD LTGIFMHGKI PTLKISLIQI FRAHLWQKIH ESVVMDLCQV FDQELDALEI ETVQKETIHP RKSYKMNSS CADILLFAAY KWNMSKPSLM ADTNDVYDQK PGNKYWVDVQ LRWGDYDSHD IERYTRAKFL DYTTDNMSIY P SPTGVMIG IDLAYNLHSA YGNWFPGIKP LVIQAMAKIM KSNPALYVLR ERIRKGLQLY SSEPTEPYLS SQNYGELFSN QT VWFVDDT NVYRVTIHKT FEGNLTTKPI NGAIFIFNPR TGQLFLKIIH TSVWAGQKRL SQLAKWKTAE EVAALVRSLP VEE QPKRII VTRKGMLDPL EVHLLDFPNI VITGSELQLP FQAAIKLEKF GDLILKATEP QMVLFNIYDD WLKTISSYTA FSRL ILILR ALHVNPEKAR MILRPDKSIV TQPHHVWPSL TDEQWIKVEV ALKDLILADY GKKNNVNTQA LTQSEIRDII LGAEI TPPS QQRQQIAEIE KQAREGGHMT AVTTKTTNVH GDDLIVTTTS PYEQAAFGSK TEWRVRAISA ANLHLRVNHI YVNSDD IRE SGYTYVLPKN LLKKFICIAD LRTQIAGYMY GVSPPDNAAV KEIRAIVMPP QWGNHQLVNL PANLPEHDYL KDLEPLG WI HTQPNELPQM SPQDVVATAK MLEAHRSWDG ERCVCVTVSF TPGSCSLTAY KLTPGGYEWG RSNKDVAANP QGYKPDFY E KSQLLLSDRF MGFYMVPDAG SWNYNFMGVK HSPAMRYGLK LANPREFYHE VHRPTHFLEF STLEDADPGV DVENHFA

UniProtKB: MPN domain-containing protein

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Macromolecule #3: Elongation factor Tu, chloroplastic

MacromoleculeName: Elongation factor Tu, chloroplastic / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 109.366844 KDa
SequenceString: MAEDMYDEFG NYIGPALEDS EEEEEEHQVQ HGFGGSEGAD DEDGAPGPGD AMQEDDDEGG PGMAVVLHED KKYYPSAEEI YGAEVETLV MEEDAQPLEV PIVAPVKVKK FETLEAEPLR THYSNEFLAT LMANPELVRN VAVVGHLHHG KTTIMDMFVE Q THELRKMT ...String:
MAEDMYDEFG NYIGPALEDS EEEEEEHQVQ HGFGGSEGAD DEDGAPGPGD AMQEDDDEGG PGMAVVLHED KKYYPSAEEI YGAEVETLV MEEDAQPLEV PIVAPVKVKK FETLEAEPLR THYSNEFLAT LMANPELVRN VAVVGHLHHG KTTIMDMFVE Q THELRKMT AAQQAAGKQL RFTDTRLDEQ ARAMSIKMMP MSLVMESHSG KSFLLNLLDC PGHVNFNDEV TAAMRLADGV ML VVDAAEG IMVVTERAVR QALQEGLSIT LMISKVDRLI TELKLPPADA YHKLRHTIEE VNNLIAACCG GEVPPEQLLD PVR GNVTFS AAQSGWSFSL QSFARLYAEI YGAGFDPREF AKRLWGDLYF VPEDRVFRRT PPRAGVERSF VQFVLEPLYK MYSA VIGEH PKTVEGLLAG LGVYLRTATY NLDTKPLLKE VCSSVLGSCA GVVDMLVAHV PPSRKASADK VAAHYSGPSD APAVS FMSA CNPRGPLVVA VAKLFPRQDC SRFDALGRVM SGTLRPGDTV KVLGEAYTPE DEEDSAVATV GSVWVYQARY RVPVAR ATA GNLVLIEGVD ATITRTATLV SDAYDEPVHI FRPLRFQTRS TVKIAAEPLN PSELPKMVEG LRKVSKSYPL CSTRVEE SG EHTIFGTGEM YLDCLMKDLR ELYAEVEVKV ADPVVAFCET VVESSSLKCF AETPNKRNKI TMIAEPLDKG LAEDIEAG N VRLDMGRKQL TEWFQSRYEW DLLAVRGLWA FGPELQGPNV LLDDSLAAET NKGLLNAVRD SVIQGFQWGA REGPLCDEP IRNVKFKILD AAIAPEPIHR GGGQIIPTAR RVCYSAFLMA TPRLMEPVYY VEIQTPADCI AAIYNVLAKR RGHVTADVPK PGTPIFIVK AYLPVVESFG FETDLRYHTQ GQAFCQSVFD HWQVVPGDPL DKSIVLRPLE PAPVQALARE FMVKTRRRKG M SDDVSINK FFDDPMLLEL AKQDADLAMI F

UniProtKB: Elongation factor Tu, chloroplastic

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Macromolecule #4: U5-40K

MacromoleculeName: U5-40K / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 39.437363 KDa
SequenceString: MAEKRERSQS PGPEVDAEID GDTGAIVVSK KPRTDAQLVV GSVTKEGITR TSNLSAPIIQ LSGHAGEVFS MRFSPDGQCI ASGSFDKTI FLWRTYDENE NYNVIAGHRN AVLEVHWFTD GEALLSCSAD KTARCWDAET GAPIKKMGEH TAVVNSCCPL R RGAKVFVT ...String:
MAEKRERSQS PGPEVDAEID GDTGAIVVSK KPRTDAQLVV GSVTKEGITR TSNLSAPIIQ LSGHAGEVFS MRFSPDGQCI ASGSFDKTI FLWRTYDENE NYNVIAGHRN AVLEVHWFTD GEALLSCSAD KTARCWDAET GAPIKKMGEH TAVVNSCCPL R RGAKVFVT GADDSTVKVW DMRVKKSVHT LRSEFPVCAV AFADAGDQVY SGGVDNAIKV WDLRRGGGAD VEPSMLLKGH SD SVTGLRV SPDGGHLLSN AMDNTLREWD VRPYAPPNRC TKVFTGHMHN FEKNLLRCDY AADGSKVACG SADRMVYIWD TST RKLLYK LPGHSGSVNE VVFHSKEPIV ASASSDKTIF LGELTQ

UniProtKB: Uncharacterized protein

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Macromolecule #6: Pre-mRNA-processing factor 19

MacromoleculeName: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 6 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 54.01584 KDa
SequenceString: MFCSISGTVP EQGVVSTKSG HLFEKSLIEK YVRETGKCPV TGEPLSTEDL LPLKVNKTVK PRTAPATSIP GLLSLFHDEW DANMLELHN TRTQLHQTRQ ELSHALYQHD AATRVIARLL KERDEARAAL ADMKQQYAAD VAAAAAAPRE GGGEGGRRPK A GIPEDVIA ...String:
MFCSISGTVP EQGVVSTKSG HLFEKSLIEK YVRETGKCPV TGEPLSTEDL LPLKVNKTVK PRTAPATSIP GLLSLFHDEW DANMLELHN TRTQLHQTRQ ELSHALYQHD AATRVIARLL KERDEARAAL ADMKQQYAAD VAAAAAAPRE GGGEGGRRPK A GIPEDVIA DMTEVNATLS KGRKKRPLPD SLASADDIAA MTLAGSHPLH KTTAGGIAAI DVNPQQQGVV VTAGVDTTLQ LF DYLQTRV LASLEGHTKR CTGVSWVAAD VIVSSSADKT ARVWRAGGEG GSEWSCASVL RDHTAEVVGV TTHPSRRYFV TGS TDASWA FYDVASLTCL RQVGADEGPG EPYTCLQFHP DGLILGTGTE GKAIRIWEVK AQKAVAACEG HAGAIRSLAF SENG YHMAS ASDDCIKLWD LRKLANFKTI EPYTDGPCTT VAFDHSGQYL VVGGPVVKIY GQKQQWAELK TLSEVPKRAS ALRWG PDAR SVLVGAADHN LRVFSSPSA

UniProtKB: Pre-mRNA-processing factor 19

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Macromolecule #7: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.789166 KDa
SequenceString:
MATSAKGIGI PVKLLHEAEG HVVTVELKSG EMYRGELCDA EDNMNCQLKD VTATGRDGRV TQLAHLFIRG SRVRFFIIPD MLKNAPMFK RIDPKFKNKN MALGVGGRGR AAAVRAAAKA KTPGRGRG

UniProtKB: Small nuclear ribonucleoprotein Sm D3

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Macromolecule #8: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 8.532996 KDa
SequenceString:
MPKIAPPELK RFMDKKLSIT LNANRHVSGT LRGFDQFMNL VLDATVDEKL KVDIGMVVIR GNSIQTIEAL EPIATGN

UniProtKB: Small nuclear ribonucleoprotein G

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Macromolecule #9: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.216069 KDa
SequenceString:
MATKVQKIMT QPINLMFRFL QSKQKVQIWL FDQADLRVEG RIIGFDEYMN LVLDEAEEVS MKRKTRKTLG RILLKGDNIT LIQTTGR

UniProtKB: Small nuclear ribonucleoprotein E

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Macromolecule #10: Sm protein F

MacromoleculeName: Sm protein F / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 9.644063 KDa
SequenceString:
MAAFVPVNPK PFLQDLTGKQ VIVKLKWGME YKGYLVSTDS YMNLQLASTE EYIDGQFTGQ LGEVLIRCNN VMYLRGVPEE DEPMS

UniProtKB: Sm protein F

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Macromolecule #11: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.630687 KDa
SequenceString:
MAQAPNPTVD ELEKKEEDEY RTGPLSVLTQ SVKTNGQVLI NCRNNHKLLA RVKAFDRHCN MILENVKEMW TEIPKTGKGQ KASRPVNKD RFVSKMFLRG DSVIMVLRNP K

UniProtKB: Small nuclear ribonucleoprotein Sm D2

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Macromolecule #12: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.668932 KDa
SequenceString:
MVKLVRFLMK LANETVQIEL KNGTVIQGTI AGVDIAMNTH LKAVKLTARG KNPVSMDHLS VRGNNIRYYI LPDSLNLDTL LVDIDKPKQ RPTKVRTAVG RGRGRGRGRG RGRAR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

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Macromolecule #13: Sm domain-containing protein

MacromoleculeName: Sm domain-containing protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.745186 KDa
SequenceString: MALSRTSKLL QYINFRMRVT LVDSRQIVGR FMAFDRHMNL VLGDSEEFRR LPPKKGKSEE EREERRVLGL VLLRGEEIVS LTIEGPPPN EDMRTERNQG PGGPGIGRAV GRGMPAAAPG QAPAGLAGPA RGVGGPAPGM MMPRPQMTAP PMPRPGMPPP G MPPPGMPP ...String:
MALSRTSKLL QYINFRMRVT LVDSRQIVGR FMAFDRHMNL VLGDSEEFRR LPPKKGKSEE EREERRVLGL VLLRGEEIVS LTIEGPPPN EDMRTERNQG PGGPGIGRAV GRGMPAAAPG QAPAGLAGPA RGVGGPAPGM MMPRPQMTAP PMPRPGMPPP G MPPPGMPP GMPPGMPPPG MPPPRPGMMP PPGMMPPPGM PPPRPGMPPP GMPPGMPPPG MPPPGMPPGM PPPGMPPPRP GM MPPPGMP PPGMPPPGMP PGMRPPGQ

UniProtKB: Sm domain-containing protein

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Macromolecule #14: Syf1

MacromoleculeName: Syf1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 104.377602 KDa
SequenceString: MALVATDAQK KAMEDLLPNQ EDLLYEEELA RNPYNLKMWI RYIHARADAV PKRRYLLYER AVRALPGSYK LWHAYLTERR LAVRGLRPD DVAYEALNNT FERALVSMHK MPRVWLDYLQ LLMDQRLVTR TRRTFDRALG SLPITQHDRV WALYLKFIHT P GIPVETAV ...String:
MALVATDAQK KAMEDLLPNQ EDLLYEEELA RNPYNLKMWI RYIHARADAV PKRRYLLYER AVRALPGSYK LWHAYLTERR LAVRGLRPD DVAYEALNNT FERALVSMHK MPRVWLDYLQ LLMDQRLVTR TRRTFDRALG SLPITQHDRV WALYLKFIHT P GIPVETAV RLYRRYLKLE PTHAEEYVAY LKAKNRWGEA ARKLAELLND DTFRSLEGKS KHQLWLELCD MITKHPKEVE GM RVDAIIR GGIRRFTDEV GRLWTSLADY YIRRGMFEKA RDVYEEGLCS VITVHDFSLI YDAYTQFEES LLSASMEQLA GDD EDEEGG KEKEGDDGTD FLLKDDGNDV DLRLARLEHL MNRRPELLSS VILRQNPHNV AEWHKRVKIF TGKPTKQILT YTEA VRTVD PDKAIGKPFT LWCAFAKFYE HHGDVANARI IFQKATEARH KYVDDLAHVW CEWAEMELRH SNFKRALDVV TRAIT PTPR PARMTPEEER ALPVADRVYR NLKLHLMHTD LEESLGTRES TCAAYDRILE LRIATPQVIL NYALFLTEQK AFEDAF KVY ERGIALFKYP HVKDIWTAYL AAFVERYGGK KLERARDLYE QAIKDAPPTE CKPLYLEYAK LEETYGLARH AMDIYAR AL AAVPKAERKS VLDLYVSRAS DFFGIAKVRE IYESAIEAEP PHELSDDDVR EVCMRYSALE TKLGEIDRAR AIYVHGSA V SHPDRAADFW AAWRAFEVRH GNEDTFKEMM RILRSVKVSF SHTQINSIVD AARLTSKADA EAGAGGAVAG AKRPRDGGD AMAELEAQAV AEMGLGAGAA AGGAGRGTAL SGFVSAGVYQ QGADKDKEGG TAAAPQQRKP APVAANPEEI DLDADADDDG AGAGGAEDM DVAEKAVPAG VFGSLAGLAG KGQGEGGEAA AKKQRT

UniProtKB: Uncharacterized protein

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Macromolecule #15: Crooked neck protein

MacromoleculeName: Crooked neck protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 96.708734 KDa
SequenceString: MSDPKGQLPR TTRVKNKQPA DKQITAEQLL REAKEIQLED DYKAPKQIIT DPEELAEYRL SKRKQFEDLV RRVGRFNGGV WVKYATWEE QQKDFRRARS VWERCLAIEY RNVSMWLKYA EMEMRHRFVN HARNVWDRAV SLLPRIDQLW YKYIHMEEML G NVAGARQV ...String:
MSDPKGQLPR TTRVKNKQPA DKQITAEQLL REAKEIQLED DYKAPKQIIT DPEELAEYRL SKRKQFEDLV RRVGRFNGGV WVKYATWEE QQKDFRRARS VWERCLAIEY RNVSMWLKYA EMEMRHRFVN HARNVWDRAV SLLPRIDQLW YKYIHMEEML G NVAGARQV FERWMRFEPD HTGWMAYIKF ELRYNEVDRA RAIFERYIQI LPTVKAWVRY AKFEMQNGEV GLARRCYERA VD ELGEDAQ TEEFFIKFAE FEEKAREVER ARAIYRYALD HIPKASAPSL YQRFVAFEKQ HGDREGIEQV VVSKRRFQYE EDI AKSPYN YDTWFDYIKL EEGTGDIERT REVYERAVAQ LPPSSAEKRF WRRYIYLWVK YALFEELDCA DPDRTRDVYR AVLD LIPHR QFTFAKIWIM AAKFEIRQRN VEGCRKLLGR ALGLCPKEKL FKAYIELELT MGNVDRVRKL YEKYLEWRPS NVGAW VRFA DLERQLGETG RARALYELAI GQPLLDMPEA LWKSYIDFEI AAGERERVRV LYTRLLDRTK HVKVWLSFAR FEATPM PAE GAEGEDGQPA EPAEGPESAP ARAARARSVY DRAFTTLRDV SPDSKEEAVL LLEAWRDFER EQAWSSEADR AARVSEV EK KMPRRVKRKR PVTTEDGHEV GQEEYYDYIF PEEGAAAPGL KLLEAAYRWK RQRQGAAPGE EPQAQQGRGG AGEEAPSA S DAAQAFAAAA MSRFAGAGEK SGSEEPRQEE EQEQDGRPAR GGRRGDEEGD EVEEGRARGR EGGDEEEKQE EGVRAGEVE EGRPGRGHGS QAGEAGVEGE GPGAAGGEAM AGEDS

UniProtKB: Crooked neck protein

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Macromolecule #16: Cwf15/Cwc15 cell cycle control protein

MacromoleculeName: Cwf15/Cwc15 cell cycle control protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.422254 KDa
SequenceString: MTTAHRPTWA PAIGGEEQGG MRIFKPSVQQ SAKNLPGHTK LKFRQTGQAA EEELRAKDLR AELEAKERKH FGKQSGTDTS FEDERKRDL ELLQSAPPEG GARQLIPKAI DADDEDPESS ESSDDDDDDD EKALLLAELE RIKKERAEDA AKKAREEADV A AKAREEEL ...String:
MTTAHRPTWA PAIGGEEQGG MRIFKPSVQQ SAKNLPGHTK LKFRQTGQAA EEELRAKDLR AELEAKERKH FGKQSGTDTS FEDERKRDL ELLQSAPPEG GARQLIPKAI DADDEDPESS ESSDDDDDDD EKALLLAELE RIKKERAEDA AKKAREEADV A AKAREEEL RAGNPLLAIG AAGGAAGADV SFNIKRRWDD DVVFKNQARG EPKQQKRFVN DTIRNDFHRR FLQRYIR

UniProtKB: Cwf15/Cwc15 cell cycle control protein

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Macromolecule #17: PPIase cyclophilin-type domain-containing protein

MacromoleculeName: PPIase cyclophilin-type domain-containing protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 61.094574 KDa
SequenceString: MENPRVWFDI QIGSEAAGRV VMELYADIVP KTAENFRALC TGEKGVGKSG KRLHFKGCVF HRIIPEFMCQ GGDFTASDGT GGESIYGAK FADEAPGLAL KHDKPGILSM ANAGANTNGS QFFICTVPTP WLNGKHVVFG RVVEGMTTVK RMEVVGQRSG K PSRKVIIT ...String:
MENPRVWFDI QIGSEAAGRV VMELYADIVP KTAENFRALC TGEKGVGKSG KRLHFKGCVF HRIIPEFMCQ GGDFTASDGT GGESIYGAK FADEAPGLAL KHDKPGILSM ANAGANTNGS QFFICTVPTP WLNGKHVVFG RVVEGMTTVK RMEVVGQRSG K PSRKVIIT DCGQLPSKLQ MMLKLKAEKE EAAKLKSDPN ELNPDQDSLA RLKALQQQTE QQQAAKKARK APAGALGSIA AS LLGIGKE GAAGSEAAAA PAEGGEKRKR KARDASAEPE AEQQQQQQQQ QGGEEMEMEQ EERQHGGAGA GPGPGSEGGE EEG AGGAGG GVVVEGADPF AGLSAKQRKL MELRQRLQVC RKQNQGAVIA EKKRQKSPGA EAAADDSASG QRRWHEEKQR KRAE ELARL GLDPTQAHRL ETAETAALKY EKRDKKGGPT GWGVFAPENL YQAHLKRAEA GVAAAYSVED YERAKAADPE FYRGA DSLK YGQQPKLPAA NVEKMVAELQ ERDRKKDEFS RRRAHNDGSD VDFINDRNAH FNKKIQRAFG QYTTEIKGNL ERGTAL PDR

UniProtKB: peptidylprolyl isomerase

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Macromolecule #18: PLRG1

MacromoleculeName: PLRG1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 56.10048 KDa
SequenceString: MAAETTSEVV IESFNPDLSA DAKTIKALTL QSLKRTYEVF ASNYGQPVPL DETSQLIKAA VKFRDEYEHV SHLVAPAPAP KPTAAPPPG PSGRTAAAAG AAGPSADGDA SRPESRSNLA KLIDNIPAAV AKKLGDAPGA GQLTLYQPPG GPAAPGADAE A HQRAVMAV ...String:
MAAETTSEVV IESFNPDLSA DAKTIKALTL QSLKRTYEVF ASNYGQPVPL DETSQLIKAA VKFRDEYEHV SHLVAPAPAP KPTAAPPPG PSGRTAAAAG AAGPSADGDA SRPESRSNLA KLIDNIPAAV AKKLGDAPGA GQLTLYQPPG GPAAPGADAE A HQRAVMAV IADKGGNSSA AVSRRIASKW PRPVWHAPWK MYRVISGHLG WVRCVAVDPS NEWFATGSAD RTIKIWDLAS GQ LKLTLTG HIEQVTGLAV SSRHPYMFSC GLDKMVKCWD LEQNKVIRSY HGHLSGVYSI ALHPNLDVLM TGGRDSVVRV WDM RTKVQA MVLSGHDQTV CSLLAQAPDP QVISGSHDST IRLWDLRKGK ASAVLTHHKK SIRALAMHPH EFAFASASAE NIKK WALPD GDFLHNMLSQ QRAIINSMAI NQDGVVATGG DNGSLWLWDW RSGHCFQQDE TVVQPGSLES EGALYDMTFD MTGSR LITA EGDKTVKMWK EVEDATPETH PGLPFRPPKD IKRF

UniProtKB: Uncharacterized protein

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Macromolecule #19: Rbm22

MacromoleculeName: Rbm22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 44.413184 KDa
SequenceString: MAARLLRDPE ADGWERSDMP IVCETCLGPN PFVRMQRIEF GGTCHISGRP YTVFRWRPGN DARYKKTVIC QEVAKAKNVC QVCLLDLEY GLPVQVRDAA MGVKPDEEPQ SEVGKEYKLQ MEADAGTLGG GGVGGASSSY AAGRPNEMLQ KLQRSQPYYK R NQARVCSF ...String:
MAARLLRDPE ADGWERSDMP IVCETCLGPN PFVRMQRIEF GGTCHISGRP YTVFRWRPGN DARYKKTVIC QEVAKAKNVC QVCLLDLEY GLPVQVRDAA MGVKPDEEPQ SEVGKEYKLQ MEADAGTLGG GGVGGASSSY AAGRPNEMLQ KLQRSQPYYK R NQARVCSF FAKGQCTRGA ECPYRHELPT ADPALANQSY KDRYYGTNDP VAAKMLKRVD ELNKLTPPED TSITTLYVGG VD ASITEDD VRDAFYSFGE LASVRKMDVK SCAFVTYTTR SAAEKAAEEL GGNPLIKGAR VKLMWGRPPP APAARNAAAA DPM QPSTSG AGGYGGAAPG SAASYYPSMD PSAMGSRAPG GPPGMRPGGE GGGPGGPGGM APPRPMGYGA PPGYGAPPPG YMPP PRPMV SASMQPPQQQ HQ

UniProtKB: Uncharacterized protein

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Macromolecule #20: G10 protein

MacromoleculeName: G10 protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.355854 KDa
SequenceString: MSLRRKLKGK KPPEGWELIE EVIEDFEQQL KEAVNEEHEG KRKTELTWKI HRLHWEKNRF IYDLMYQRKV MSKELFEWLV REKVADGAL IAKWRKPGYE ILCSMLAIQK GNHNFGTTSH CRVPLRARAK QQRITPDVQT GCISCASGDG KFGGPVWWNT P LDEEDSAK ...String:
MSLRRKLKGK KPPEGWELIE EVIEDFEQQL KEAVNEEHEG KRKTELTWKI HRLHWEKNRF IYDLMYQRKV MSKELFEWLV REKVADGAL IAKWRKPGYE ILCSMLAIQK GNHNFGTTSH CRVPLRARAK QQRITPDVQT GCISCASGDG KFGGPVWWNT P LDEEDSAK LEENRTTWGQ AGTGEPGDGM GGGGGGPGPS RKRPASTLDG DDDDELPDEV RARLEALKKG TKALD

UniProtKB: G10 protein

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Macromolecule #21: SKI-interacting protein SKIP SNW domain-containing protein

MacromoleculeName: SKI-interacting protein SKIP SNW domain-containing protein
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 73.924797 KDa
SequenceString: MSLLGTLPAP QQKHAAPIAP VPASAAIVSA SFKEPPPYLR RKGWVPRRAE DFGDGGAFPE IHVAQYPLDM GKGSTGGKQQ LAISVNEKG EINYDAVVKQ GQNKDRVVHS DHGALVPKVD RLSKEALARP DEEDVEKTVA ETAAALERVV QGKLSSVNPT K LPGGPGGS ...String:
MSLLGTLPAP QQKHAAPIAP VPASAAIVSA SFKEPPPYLR RKGWVPRRAE DFGDGGAFPE IHVAQYPLDM GKGSTGGKQQ LAISVNEKG EINYDAVVKQ GQNKDRVVHS DHGALVPKVD RLSKEALARP DEEDVEKTVA ETAAALERVV QGKLSSVNPT K LPGGPGGS TLIKYTPAQQ GAQYASGAGQ RIIKMQDLPV DPLEPPKFRH VKVPRGPG(SEP)P PVPVLH(SEP)PPR PLTV KDQQD WKIPPSISNW KNPKGYTIPL DKRLAADGRG LQQTQINDKF AALSEALFTA EAKAREAITM RASIQKELAL KEKNK RETE LRNLAMQARM ERQGAPGAGA GAGGLPPPPP LAGRAEGSLP GPPPGPPPRG AGGRATAADA FDDHEEDYDR RREPEP ERE RERERDRGGD RERGERDRDY DRDYDRGERG GYDRERERDA DRGGRGGGED ERYQESVAER EERRRRDEIR EERRRER ER ERRLEAANQG GAKKSKITRD RERDISEKVA LGMANVGGGG EVQYDQRLFN QDAGMQSGFG ADDSYNAYDK PLFADRGS H LFKASRATAD DDEDAGGAAA GGDAGPRTER FKPDKGFQGA EGAPGAGGGR GGGGRLEFER QQAEEADPFG LDQFLSEVK GGGRDRGGKG NALDAIGKRG GMSAAGGGAS LDDLAGAGAG MGRRMQFTSG KG

UniProtKB: SKI-interacting protein SKIP SNW domain-containing protein

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Macromolecule #23: Peptidyl-prolyl cis-trans isomerase

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 17.30967 KDa
SequenceString:
MEAPLVQIDT SMGSFTVELY YKHAPRTCKN FEELAKKGYY DGTIFHRIIR DFMCQGGDPT GTGRGGESIY GGKFEDEITR DLKHTGAGI LSMANSGPNT NGSQFFVTLA PTPWLDGKHT IFGRVTSGMA VIKRLGNVQT DNLDRPVTEV KVIRARPV

UniProtKB: Peptidyl-prolyl cis-trans isomerase

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Macromolecule #24: Prp17

MacromoleculeName: Prp17 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 63.995871 KDa
SequenceString: MDLLASYGDD EDRSPLDAPE SPTAAAIAAS APKLNLSISA APQVDTTGLV LRGNEAKSLG EVRKLQDAGA RKVMYNLPVE DMHAPVLGP AHPYSRDGLA AGLKNHFVGH VEDAHMHPIH FEQQYHNFHA LGFGEAPSGI GVVGKPVDED AVEAAEEGGP S AWKRRKTT ...String:
MDLLASYGDD EDRSPLDAPE SPTAAAIAAS APKLNLSISA APQVDTTGLV LRGNEAKSLG EVRKLQDAGA RKVMYNLPVE DMHAPVLGP AHPYSRDGLA AGLKNHFVGH VEDAHMHPIH FEQQYHNFHA LGFGEAPSGI GVVGKPVDED AVEAAEEGGP S AWKRRKTT QERKAEQVAR AERLAAPFDP SAPFSLQERQ PWASKQAEIA ELTEEQKEFI AKAEAAKLEA AAGSVDKQGP TS FFHAKEE KDYQGRSWIL PPRDKKNESD SCFLPKRWVH TWAGHTKGVN AVRFFPGTGH LLLSAGMDGK VKIWDVYGSG KCM RTYLGH SKGVRDVCFS NDGRRFLSTG YDKNIRLWDT ETGQCIKSFN TGKVYYCVKF HPSDDKQNVF MSGCQDKKIY QFDT DTGES VQEYNYHLGP VNSVTFIDDG RQFVSTSDDK TIRVWEFGIP VQIKYIADPS MHSMPVVATH PSKNYLLMQS LDNQV LTYM VKDGRFKSMK NKTFKGHNTA GYACQVGCSP DGKYVMSGDS EGRCFFWEWG APQKIVRTIK AHDAVCIGAE WNPMES SKV ATCGWDGLIK YWD

UniProtKB: Uncharacterized protein

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Macromolecule #25: Cdc5L

MacromoleculeName: Cdc5L / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 91.838617 KDa
SequenceString: MRIMIKGGVW KNTEDEILKA AVMKYGLNQW ARISSLLVRK SAKQCKARWY EWLDPAIKKT EWTREEDEKL LHLAKLMPCQ WRTIAPIVG RTPAQCLDRY ERLLDQAVAK DVNYDPRDDP RRLRPGEIDP NPEAKPARPD AVDMDEDEKE MLSEARARLA N TRGKKAKR ...String:
MRIMIKGGVW KNTEDEILKA AVMKYGLNQW ARISSLLVRK SAKQCKARWY EWLDPAIKKT EWTREEDEKL LHLAKLMPCQ WRTIAPIVG RTPAQCLDRY ERLLDQAVAK DVNYDPRDDP RRLRPGEIDP NPEAKPARPD AVDMDEDEKE MLSEARARLA N TRGKKAKR KAREKQLEEA RRLAQLQKKR ELKAAGIDVR ARNKNSRAVD YSSEVAFELK PQAGFYSTAD EEKTTRSMQQ EF RPVTVEE LEGRRRKDIE EALIKKDVKR QKINEMHDAP NAVAKHMGLD QEGPRRRGKL MLPAPQVSEA ELEQIARLGV DGA LEASVS EGAGGDATRT LLGQYNQTPA ARLGMGPGAT PMRTPRAGPG GSDRIMAEAA ALARLQGMGT VLEGGEAGVD VAAM DFAGV TPRNVVAATP NPLAGMTPSV RGAGGATGAR VVPSIAGVSA TPSVAGTPLR AGSGGVGPGA TPLIRDALGL NEADA LTME AAMSKRAAAA RAAVMRGELR ERLAGLPTPQ NEYAFEVPEL PEAEEEAEDA MEEDLADVRA RKAREEAERR RIEELK KSK ALQRQLPRPL NLDSLPAPKP AAPSTAGASA EQLRDRAEEL VAAEMRGLLQ HDAAKYPVKD GRDARKKGKQ QHAAPGP VP VLEEFELEAL QSAAELVDRE VAYLRSAWDH AKLSPDDYSE VWMSVHRDLI YLPSRQRYER AASATNVDRI DSLKSEFD N VRADMEREAK KAAKLEGKLG LLLGGLQRRH GDLTGRVGEL WAQVRDAAQE LVCFKALHER ELRAAPERLE ALGELVDAT KRREVDLQER FKALTRRRDE LAAALAQKRA AAS

UniProtKB: Uncharacterized protein

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Macromolecule #26: Pre-mRNA-splicing factor SPF27

MacromoleculeName: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 32.563047 KDa
SequenceString: MAAKTSQATL ALEYTAHGAA KGWRANEHLV DALPYVDSVP PELKPHVEAL IEEEKRRSTK LPSDYLREMP SVRAPKFDDH PVLKTEYER VRNKEPMAPL DSVRYRLEPP PQARRGDVGA WKSSLDNAAA QLEHQHLRIL NQELLLKYGD KAWRAQVALD E AAVRGLEA ...String:
MAAKTSQATL ALEYTAHGAA KGWRANEHLV DALPYVDSVP PELKPHVEAL IEEEKRRSTK LPSDYLREMP SVRAPKFDDH PVLKTEYER VRNKEPMAPL DSVRYRLEPP PQARRGDVGA WKSSLDNAAA QLEHQHLRIL NQELLLKYGD KAWRAQVALD E AAVRGLEA QLAALRKETD GLNRERKLQQ HAAGSELSKL ERQYLSQVRK NADIERACDR LEDAVAAMEA ELDTHIRADT GA EAEAEAG AAEGQQGPGA AGEAEAAGAD KPAAAADGSG DTDMADGAAA AEGAAANGTH AAGAES

UniProtKB: Pre-mRNA-splicing factor SPF27

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Macromolecule #27: CWF21 domain-containing protein

MacromoleculeName: CWF21 domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 80.630266 KDa
SequenceString: MYNGIGLVTP RGSGTSGYVQ GNKFNMRGPP QVRTDAPKDG GPRVKQPNQE ILDHKKKRDI ELKVEQERER LEAEGVHSEE EIEELLGLF RQQELAKLTA EANTVKKLDE ETHAVAQRKL EQMSKLKAAL GLKEDVREGD AFNRELQEKI KQDRIAEREK R ERDREADI ...String:
MYNGIGLVTP RGSGTSGYVQ GNKFNMRGPP QVRTDAPKDG GPRVKQPNQE ILDHKKKRDI ELKVEQERER LEAEGVHSEE EIEELLGLF RQQELAKLTA EANTVKKLDE ETHAVAQRKL EQMSKLKAAL GLKEDVREGD AFNRELQEKI KQDRIAEREK R ERDREADI KRREKEARRR EKEWAKEQKR REKERKQREK ERREEEERRR NASPEARLYR KHEAAPDRYD PAADREAEQR QR ERARAGR DDRDSGRGDA AERGRSKDAK GPKDGKGKEK GGKEKEEKRR RRRRSPSSSS SSGSSSGSDS DSESGSSGSS SGS GSDSDS GTSSGSGTSS SSSDSEDRGG DRRQARSDPS GDRGAKRGPA DRGLPPPPEP EPKRARNGDG PPPPRDLPLP PPPA AAGRP PAGDDRRRDA GERDRLAERA PEGRRRHDSD DEREQARNVR ARREEEEERG RGREEPGPAR RDARDAGGDR DGGRR PPPP PSREVDREPE RREREREPEP DRRERRDLPP PPAAPSGRER DARDVRDARG DGHRADFERP RGREQPDRER ERERGR EDG AGREDRGGDG RGGDRVGHRG NGREEERPAR GGDRGGDRGG DRERRGGEEP PARGGPWRGE EERRRGGDRE REPERAR EP ELGQGRERER ERAGDGGDRG APARGRDERE RDAGRRRRSP SPSSSSSSGS SSGSGSSSSG SGSSSSSGDS SDSASSGR R

UniProtKB: Uncharacterized protein

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Macromolecule #28: MI domain-containing protein

MacromoleculeName: MI domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 102.389445 KDa
SequenceString: MAPRSDDEDG RRGGGSRRKD RRSPSPSSSG SSGSSSSSGS SSSSDSDSEP PRRDRGSDRG PDRRESERER EREERPAGDR PSGADKQRA EGEDKDAAGA SDAAPPAAGP DAPKESERAA GDDKAAAEAG PDGDQPRRSV FDRLGGGAAG GGGSGRDRER G GDRDEDRY ...String:
MAPRSDDEDG RRGGGSRRKD RRSPSPSSSG SSGSSSSSGS SSSSDSDSEP PRRDRGSDRG PDRRESERER EREERPAGDR PSGADKQRA EGEDKDAAGA SDAAPPAAGP DAPKESERAA GDDKAAAEAG PDGDQPRRSV FDRLGGGAAG GGGSGRDRER G GDRDEDRY RSGDRDRDRY EPERDRRRGD DDRYRRGGSY DDRDRRDRDL DRDRRDRDRD RGYDRDRERE RERDRERYRD DD RDRRDRD RDRSRERDRS PRKRRRDDEA DEPRREPAAD AADAPANGGA VDAGAAAGAE GAEAVARKPV ALGKTGGVYI PPF KLARMM AEQGAQDKAS PEYQRLTWEA LKKSLNGIIN KINTVNIKNM LPEVFRENLI RGRGLFCRSL MKSQAASPGY SPVY AALVA VVNTKFPELG ELLLSRLILQ FKRSFKRNDK PVCTAAAKFL AHLVNQGVAH EVLALEVLIL LLEAPSEDSV EMAVE FVRE VGAHLQDVAP QGLHGVFERF RAILHEGSIS KRVQFIIEGM FALRKAGFEA SGFPALKPEL DLVEGEDQIT HEMGLD DAL QAQTNLDIFR VDPDYEEEER KYAVIAKEIL GDEEEEEGGG GAEGAAGEAA DDEDSSDDDD DDEDGGDPGS GGGGGSG GG GIHDATATNL VNLRRTIYLT LMSSFDFEEA GHKLLKIGIQ PGQEIELVTM IIECCSQERT FKRFYGLLAQ RFCYLNRA Y AEQFEDCFRK QYAVIHRLET NKLRNVSCLF AHLLATDALP WSSLSCIMLT EEETTSSSRI FIKYLFQELS STMGLVKLN QRLNDPAFSP WFEGLFPRDS LGNMRFSINF FTSIGLGGVT DVMREQYKSA VAAQAAAQQQ QVAKRMLGSG SGSDSGSSSS DSSSSDDSS SDSSSSSDSD SDDSRGRRRS KGKGKGKKSD KKKKSSKRR

UniProtKB: MI domain-containing protein

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Macromolecule #29: Intron-binding protein aquarius

MacromoleculeName: Intron-binding protein aquarius / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 201.450953 KDa
SequenceString: MADTDKAPEP MAEDDQPQQQ PENETPDAAT EAPPAEGASE EPAAKKARTA PKFVPKQFQV SGPMQGRGQG RGFGRGQQFM GPMGAAGRF GWPRPQFMPQ QQQQQPQRPR GGLTLTEIAE DKLTKTAKQE WSNEARAQEN PPAFKPELVQ TIYKQELGGA S KRAPGHKR ...String:
MADTDKAPEP MAEDDQPQQQ PENETPDAAT EAPPAEGASE EPAAKKARTA PKFVPKQFQV SGPMQGRGQG RGFGRGQQFM GPMGAAGRF GWPRPQFMPQ QQQQQPQRPR GGLTLTEIAE DKLTKTAKQE WSNEARAQEN PPAFKPELVQ TIYKQELGGA S KRAPGHKR IMLLEISQYL ENYLWPNFDV EKATFEHVMS LVLMVNEKFR EGVPAWTCFH TREDAFPGFF KRVLSLKDGR EE ELKLHER TAYVLFMIRS FQSLEDEMVR AQVLRLVSLP LWHALSPGRL QLELHAHEAL AKHWKAAAKK EAKAAAKKAK KGE EGGEGG SYVPVTQRPE TRFLPALMDE FLAVLDQVVV PPKLPGCYPS LNRGALLYCE RFLEFLIDLL SQLPTRRFVR TLID DRQLL VKVRMSGLFK YEVPPPGAAA AALANAKAAA RAAARAAKAA ARRAAKEAAK ENGAEEAEDA EDAEEAEEEQ DDGAS DSGS LGDGDAGGDS SSEDGGDAGG EGGKGEEGGE GGAGEEGSEV AAAEDDAAAP AEAAGDERPA AEANEDAAND AAAAGP AST PSKEGEGKDA AAGGQGRGRG RGASRGRGGR GRGGRSPGRG GRGRGRGQGR GASAGRRRGR SAAEGDGSGR GPAPPPS NL LYRQLVDLFS YYMSFPINDH TGEPLTDDEV NAAHYEKVCQ FQRLCFKHWQ GVEAMQELAL SHCGAVEARD TLRRHLAS L TGEQVRELVC RQLRLVGEDD PWAADGAFLL EVLLAAYERR RSQREVVNEM PLYPTEGLLW DESQIPASSE HYTGEGALA LPKLNLQFLT VADYLLRSFH LFRLEATYEV REDLADVLGR VGAYTGDDGR TRFAGWARMA LPLTSFKVTE VRKPNVGEAK PAGVTANVV IDTRPLRGDV RSEWDELKQH DVLFLLTIRP PDAHAAAAAH ADGRTPNPAE KFGLVYVRGC EVIELRDEGG K LMNDFTGR VRRDEVKPPE GTARTVTVAL DTAQYQIDMN TMARHKSEDP YATFNLLMRR KPKENNFKAV LESIRDLMND DT AVIPPWL HDVFLGYGDP AAAQARVPGT AAAAAAAAAE GGTGGEGVGG QAPLRTVDFG DTFLDAQHVV EAFPQFKVSF VNK SGKAVP APPFRITFPT QPLPEDASTS GADAAAAAAE AAAAGSFPAP ADPANPAAGE LVVEAYVPPD PGPYPQDQPR RNAV RFTPV QVEAIASGVQ PGLTMVVGPP GTGKTDTAVQ VMTCLYHNCP GQRTLLITHS NQALNDLFSK IMERDVPERY LLRLG MGEA ELDTEQDFSR VGRVNAMLAR RLELLAEVEK MARQLGVPEA ESVAYTCETA GYFWLIHVLA RWEKFTAAVE RARAGG AGA AVIAELFPFK EYFADAPQPV FAGASFDADM ERARGCFRHL KTLFQELEEC RAFEMLKGQA DRVNYLSTKQ AKIVAMT CT HAALKRREFL QLAFKYDNLL MEEAAQILEI ETFIPMLLQK PEDGVSRLKR VVLIGDHHQL PPVVKNQAFQ KYSHLDQS L FTRFIRLGTP YVQLNMQGRA RASLAQLYNW RYKALGDLPA VQALPAFRAA NPGFVHEYQF VDVPDYLGRG ESEPLPYFY QNLGEAEYVV ATFMFMRLLG YPAHKISILT TYNGQKALIR DVIEQRCAPY PMFGRPYRIA TVDKYQGAQN DYILLSLVRS RAVGHLRDV RRLVVAMSRA RLGLYVFGRK ELFANCYELK NTFRLLMARP TKLALVKGES YGVCSRQVDD PVAQPDLMDG V EAMSGLVA AITEEQTAAA YAAYQTQVPA QAAEGEQGQA QAEGQEDKPM AEAAGEEVKD KEGEEAAKPE GRETVEGKAG AA VMEE

UniProtKB: Intron-binding protein aquarius

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Macromolecule #2: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 35.37075 KDa
SequenceString:
AUCCGCGACG AGCUGAACGC GUAGCGAAUA UCUCUUUCGC CUUUUACUAA AGAGAUCCGC GAGUUUUGCU CACGCAGUCG CAUAUCCAU CUUUUUGUGG GGCUUUUGCC UU

+
Macromolecule #5: U6 snRNA

MacromoleculeName: U6 snRNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 32.76043 KDa
SequenceString:
(G5J)UGCUUCGGC ACAACUGUUA AAAUUGGAAA AAUACAGAGA AGAUUAGCAU GGCCCCUGCG CAAGGAUGAC ACGCAU AAA UCGAGAAAUG GUUCCAAAUU UU

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Macromolecule #22: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 61.386055 KDa
SequenceString: AUACCUUUCU CGGCCUUUUG GCUCAGAUCA UGUGUAGUAU CUGUUCUUAU AAGCUUAAUC GCUUAUAUGG UUCUCAUUGA GAACCUGCG AUUAACUUUA AUAUUUUAGG GGGUCAAGGC AAUGGGGGCG CUUGCGUCCC GCCGCGACAG CUUGGCAGGA G GCUUACAC ...String:
AUACCUUUCU CGGCCUUUUG GCUCAGAUCA UGUGUAGUAU CUGUUCUUAU AAGCUUAAUC GCUUAUAUGG UUCUCAUUGA GAACCUGCG AUUAACUUUA AUAUUUUAGG GGGUCAAGGC AAUGGGGGCG CUUGCGUCCC GCCGCGACAG CUUGGCAGGA G GCUUACAC UACCUCUCUU GCCGAGCACC CCAG

GENBANK: GENBANK: X71483.1

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Macromolecule #30: RNA (5'-R(P*CP*CP*GP*AP*AP*CP*G)-3')

MacromoleculeName: RNA (5'-R(P*CP*CP*GP*AP*AP*CP*G)-3') / type: rna / ID: 30 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 2.219411 KDa
SequenceString:
CCGAACG

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Macromolecule #31: RNA (173-mer)

MacromoleculeName: RNA (173-mer) / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 55.292445 KDa
SequenceString:
GUAAGAGCCU AGCAUUAGUA CUGGUUACCU GCAGCCCAAG CUUGCUGCAC GUCUAGGGCG CAGUAGUCCA GGGUUUCCUU GAUGAUGUC AUACUUAUCC UGUCCCUUUU UUUUCCACAG CUCGCGGUUG AGGACAAACU CUUCGCGGUC UUUCCAGUGG G GAUCCGAU AUCCG

+
Macromolecule #32: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 32 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #33: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 33 / Number of copies: 7 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #34: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 34 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.9
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 518369
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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