EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ribosomes hibernate on mitochondria during cellular stress.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 8666, Year 2024
掲載日	2024年10月8日
著者	Olivier Gemin / Maciej Gluc / Higor Rosa / Michael Purdy / Moritz Niemann / Yelena Peskova / Simone Mattei / Ahmad Jomaa /
PubMed 要旨	Cell survival under nutrient-deprived conditions relies on cells' ability to adapt their organelles and rewire their metabolic pathways. In yeast, glucose depletion induces a stress response mediated ...Cell survival under nutrient-deprived conditions relies on cells' ability to adapt their organelles and rewire their metabolic pathways. In yeast, glucose depletion induces a stress response mediated by mitochondrial fragmentation and sequestration of cytosolic ribosomes on mitochondria. This cellular adaptation promotes survival under harsh environmental conditions; however, the underlying mechanism of this response remains unknown. Here, we demonstrate that upon glucose depletion protein synthesis is halted. Cryo-electron microscopy structure of the ribosomes show that they are devoid of both tRNA and mRNA, and a subset of the particles depicted a conformational change in rRNA H69 that could prevent tRNA binding. Our in situ structural analyses reveal that the hibernating ribosomes tether to fragmented mitochondria and establish eukaryotic-specific, higher-order storage structures by assembling into oligomeric arrays on the mitochondrial surface. Notably, we show that hibernating ribosomes exclusively bind to the outer mitochondrial membrane via the small ribosomal subunit during cellular stress. We identify the ribosomal protein Cpc2/RACK1 as the molecule mediating ribosomal tethering to mitochondria. This study unveils the molecular mechanism connecting mitochondrial stress with the shutdown of protein synthesis and broadens our understanding of cellular responses to nutrient scarcity and cell quiescence.
リンク	Nat Commun / PubMed:39379376 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	1.94 - 14.64 Å
構造データ	EMDB-43970: Non-translating Schizosaccharomyces pombe ribosome large subunit 手法: EM (単粒子) / 解像度: 2.44 Å EMDB-43971: Non-translating Schizosaccharomyces pombe ribosome small subunit 手法: EM (単粒子) / 解像度: 2.96 Å 43972 9axt EMDB-43972, PDB-9axt: Non-translating S. pombe ribosome 手法: EM (単粒子) / 解像度: 2.4 Å 43973 9axu EMDB-43973, PDB-9axu: Non-translating S. pombe ribosome large subunit 手法: EM (単粒子) / 解像度: 1.94 Å EMDB-43974: Translating Schizosaccharomyces pombe ribosome small subunit 手法: EM (単粒子) / 解像度: 2.84 Å EMDB-43975: Translating Schizosaccharomyces pombe ribosome large subunit 手法: EM (単粒子) / 解像度: 2.41 Å 43976 9axv EMDB-43976, PDB-9axv: Translating S. pombe ribosome 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-50266: Cytoplasmic Fission Yeast Ribosome Tethered to the Outer Mitochondrial Membrane 手法: EM (サブトモグラム平均) / 解像度: 11.4 Å EMDB-51002: Untethered Cytoplasmic Fission Yeast Ribosome 手法: EM (サブトモグラム平均) / 解像度: 14.64 Å EMDB-51030: Untethered Cytoplasmic Fission Yeast Ribosome (S. pombe) 手法: EM (サブトモグラム平均) / 解像度: 14.2 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	Schizosaccharomyces pombe 972h- (分裂酵母) schizosaccharomyces pombe (分裂酵母)
キーワード	RIBOSOME / Schizosaccharomyces pombe / protein synthesis / S. pombe / TRANSLATION / Non-translating / 60S / Large subunit / Schizosasccharomyces pombe / tRNA / mRNA