[English] 日本語
Yorodumi
- EMDB-43973: Non-translating S. pombe ribosome large subunit -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-43973
TitleNon-translating S. pombe ribosome large subunit
Map data
Sample
  • Complex: Non-translating S. pombe ribosome large subunit
    • Protein or peptide: x 40 types
    • RNA: x 3 types
  • Ligand: x 2 types
KeywordsNon-translating / 60S / Large subunit / S. pombe / Schizosasccharomyces pombe / RIBOSOME
Function / homology
Function and homology information


cytosolic large ribosomal subunit assembly / ribonuclease MRP complex / L13a-mediated translational silencing of Ceruloplasmin expression / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / SRP-dependent cotranslational protein targeting to membrane / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / preribosome / tRNA processing ...cytosolic large ribosomal subunit assembly / ribonuclease MRP complex / L13a-mediated translational silencing of Ceruloplasmin expression / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / SRP-dependent cotranslational protein targeting to membrane / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / preribosome / tRNA processing / pre-mRNA intronic binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / rRNA processing / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / negative regulation of gene expression / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily ...Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L44e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein L19, eukaryotic / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal Protein L6, KOW domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / : / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal protein L31e, conserved site / Ribosomal L30 N-terminal domain / Ribosomal protein L31e signature. / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal protein L35A superfamily / Ribosomal L39 protein / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14
Similarity search - Domain/homology
Large ribosomal subunit protein eL8 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL19B / Large ribosomal subunit protein eL21B / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein uL30C / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL18B ...Large ribosomal subunit protein eL8 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL19B / Large ribosomal subunit protein eL21B / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein uL30C / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL18B / Large ribosomal subunit protein eL24B / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL6B / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL36B / Large ribosomal subunit protein eL37B / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL14B / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein uL2C / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein uL3A / Large ribosomal subunit protein eL30A / Large ribosomal subunit protein uL15B / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL32A / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL16A / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL23A / Large ribosomal subunit protein eL18B / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL34B / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL15B / Large ribosomal subunit protein eL38A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL42
Similarity search - Component
Biological speciesSchizosaccharomyces pombe 972h- (yeast) / Schizosaccharomyces pombe (fission yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 1.94 Å
AuthorsGluc M / Gemin O / Purdy M / Mattei S / Jomaa A
Funding support United States, Germany, 2 items
OrganizationGrant numberCountry
American Cancer Society United States
EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND Germany
CitationJournal: Nat Commun / Year: 2024
Title: Ribosomes hibernate on mitochondria during cellular stress.
Authors: Olivier Gemin / Maciej Gluc / Higor Rosa / Michael Purdy / Moritz Niemann / Yelena Peskova / Simone Mattei / Ahmad Jomaa /
Abstract: Cell survival under nutrient-deprived conditions relies on cells' ability to adapt their organelles and rewire their metabolic pathways. In yeast, glucose depletion induces a stress response mediated ...Cell survival under nutrient-deprived conditions relies on cells' ability to adapt their organelles and rewire their metabolic pathways. In yeast, glucose depletion induces a stress response mediated by mitochondrial fragmentation and sequestration of cytosolic ribosomes on mitochondria. This cellular adaptation promotes survival under harsh environmental conditions; however, the underlying mechanism of this response remains unknown. Here, we demonstrate that upon glucose depletion protein synthesis is halted. Cryo-electron microscopy structure of the ribosomes show that they are devoid of both tRNA and mRNA, and a subset of the particles depicted a conformational change in rRNA H69 that could prevent tRNA binding. Our in situ structural analyses reveal that the hibernating ribosomes tether to fragmented mitochondria and establish eukaryotic-specific, higher-order storage structures by assembling into oligomeric arrays on the mitochondrial surface. Notably, we show that hibernating ribosomes exclusively bind to the outer mitochondrial membrane via the small ribosomal subunit during cellular stress. We identify the ribosomal protein Cpc2/RACK1 as the molecule mediating ribosomal tethering to mitochondria. This study unveils the molecular mechanism connecting mitochondrial stress with the shutdown of protein synthesis and broadens our understanding of cellular responses to nutrient scarcity and cell quiescence.
History
DepositionMar 6, 2024-
Header (metadata) releaseOct 16, 2024-
Map releaseOct 16, 2024-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: RCSB / Status: Released

-
Structure visualization

Supplemental images

emd_43973.png

Downloads & links

-
Map

FileDownload / File: emd_43973.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 512 pix.
= 424.96 Å		0.83 Å/pix.
x 512 pix.
= 424.96 Å		0.83 Å/pix.
x 512 pix.
= 424.96 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.83 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.38
Minimum - Maximum-1.4279369 - 3.5506682
Average (Standard dev.)0.0031022618 (±0.08045975)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 424.96 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Half map: #2

Fileemd_43973_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: #1

Fileemd_43973_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Sample components

+
Entire : Non-translating S. pombe ribosome large subunit

EntireName: Non-translating S. pombe ribosome large subunit
Components
  • Complex: Non-translating S. pombe ribosome large subunit
    • Protein or peptide: Large ribosomal subunit protein eL42
    • Protein or peptide: Large ribosomal subunit protein eL43A
    • RNA: 28S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: Large ribosomal subunit protein uL2C
    • Protein or peptide: Large ribosomal subunit protein uL3A
    • Protein or peptide: Large ribosomal subunit protein uL4A
    • Protein or peptide: Large ribosomal subunit protein uL18B
    • Protein or peptide: Large ribosomal subunit protein eL6
    • Protein or peptide: Large ribosomal subunit protein uL30C
    • Protein or peptide: Large ribosomal subunit protein eL8
    • Protein or peptide: Large ribosomal subunit protein uL6B
    • Protein or peptide: Large ribosomal subunit protein uL16A
    • Protein or peptide: Large ribosomal subunit protein uL5A
    • Protein or peptide: Large ribosomal subunit protein eL13
    • Protein or peptide: Large ribosomal subunit protein eL14
    • Protein or peptide: Large ribosomal subunit protein eL15B
    • Protein or peptide: Large ribosomal subunit protein uL13A
    • Protein or peptide: Large ribosomal subunit protein uL22A
    • Protein or peptide: Large ribosomal subunit protein eL18B
    • Protein or peptide: Large ribosomal subunit protein eL19B
    • Protein or peptide: Large ribosomal subunit protein eL20A
    • Protein or peptide: Large ribosomal subunit protein eL21B
    • Protein or peptide: Large ribosomal subunit protein eL22
    • Protein or peptide: Large ribosomal subunit protein uL14B
    • Protein or peptide: Large ribosomal subunit protein eL24B
    • Protein or peptide: Large ribosomal subunit protein uL23A
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein eL27A
    • Protein or peptide: Large ribosomal subunit protein uL15B
    • Protein or peptide: Large ribosomal subunit protein eL29
    • Protein or peptide: Large ribosomal subunit protein eL30A
    • Protein or peptide: Large ribosomal subunit protein eL31
    • Protein or peptide: Large ribosomal subunit protein eL32A
    • Protein or peptide: Large ribosomal subunit protein eL33A
    • Protein or peptide: Large ribosomal subunit protein eL34B
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein eL36B
    • Protein or peptide: Large ribosomal subunit protein eL37B
    • Protein or peptide: Large ribosomal subunit protein eL38A
    • Protein or peptide: Large ribosomal subunit protein eL39
    • Protein or peptide: Large ribosomal subunit protein eL28
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION

+
Supramolecule #1: Non-translating S. pombe ribosome large subunit

SupramoleculeName: Non-translating S. pombe ribosome large subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43
Source (natural)Organism: Schizosaccharomyces pombe 972h- (yeast)
Molecular weightTheoretical: 3.5 MDa

+
Macromolecule #1: Large ribosomal subunit protein eL42

MacromoleculeName: Large ribosomal subunit protein eL42 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 12.239647 KDa
SequenceString:
MVNIPKTRKT YCPGKNCRKH TVHRVTQYKK GPDSKLAQGK RRYDRKQSGF GGQTKPVFHK KAKVTKKVVL RLECVSCKYK NQLVLKRCK HFELGGEKKT KGAAIQF

UniProtKB: Large ribosomal subunit protein eL42

+
Macromolecule #2: Large ribosomal subunit protein eL43A

MacromoleculeName: Large ribosomal subunit protein eL43A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 10.444286 KDa
SequenceString:
MTKRTKKVGV TGKYGVRYGA SLRRDVRKIE VQQHSRYQCP FCGRNTVKRT AAGIWCCNGK GCKKVLAGGA WTVTTAAATS ARSTIRRLR EMVEV

UniProtKB: Large ribosomal subunit protein eL43A

+
Macromolecule #6: Large ribosomal subunit protein uL2C

MacromoleculeName: Large ribosomal subunit protein uL2C / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 27.151246 KDa
SequenceString: MGRVIRAQRK SGGIFQAHTR LRKGAAQLRT LDFAERHGYI RGVVQKIIHD PGRGAPLAKV AFRNPYHYRT DVETFVATEG MYTGQFVYC GKNAALTVGN VLPVGEMPEG TIISNVEEKA GDRGALGRSS GNYVIIVGHD VDTGKTRVKL PSGAKKVVPS S ARGVVGIV ...String:
MGRVIRAQRK SGGIFQAHTR LRKGAAQLRT LDFAERHGYI RGVVQKIIHD PGRGAPLAKV AFRNPYHYRT DVETFVATEG MYTGQFVYC GKNAALTVGN VLPVGEMPEG TIISNVEEKA GDRGALGRSS GNYVIIVGHD VDTGKTRVKL PSGAKKVVPS S ARGVVGIV AGGGRIDKPL LKAGRAFHKY RVKRNCWPRT RGVAMNPVDH PHGGGNHQHV GHSTTVPRQS APGQKVGLIA AR RTGLLRG AAAVEN

UniProtKB: Large ribosomal subunit protein uL2C

+
Macromolecule #7: Large ribosomal subunit protein uL3A

MacromoleculeName: Large ribosomal subunit protein uL3A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 43.889832 KDa
SequenceString: MSHCKFEQPR HGSLGFLPRK RASRQRGKVK AFPKDDASKP VHLTAFLGYK AGMTHIVRDL DRPGSKMHKR EILEAVTVIE TPPMVVVGV VGYVETPRGL RSLTTVWAEH LSEEVKRRFY KNWFKSKKKA FTKYAKKYAE STQSINRELE RIKKYCSVVR V LAHTQIRK ...String:
MSHCKFEQPR HGSLGFLPRK RASRQRGKVK AFPKDDASKP VHLTAFLGYK AGMTHIVRDL DRPGSKMHKR EILEAVTVIE TPPMVVVGV VGYVETPRGL RSLTTVWAEH LSEEVKRRFY KNWFKSKKKA FTKYAKKYAE STQSINRELE RIKKYCSVVR V LAHTQIRK TPLAQKKAHL MEIQVNGGSV ADKVEWAREH FEKTVDIKST FEQNEMIDVI GVTRGKGNEG TTARWGTKRL PR KTHRGLR KVACIGAWHP ANVQWTVARA GNAGYMHRTQ LNSKIYRIGA GDDAKNASTD FDATEKRITP MGGFVRYGVV END FVMLNG ATPGPVKRVL TLRKSLLTHT SRKALEPVSL KWIDTASKFG HGRFQTPAEA KQFLGTLKKD VA

UniProtKB: Large ribosomal subunit protein uL3A

+
Macromolecule #8: Large ribosomal subunit protein uL4A

MacromoleculeName: Large ribosomal subunit protein uL4A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 39.839129 KDa
SequenceString: MAAARPTVSI YSKDGSVSSE TIALPFVFKA PIRPDLVRSV HTAVAKNKRQ PYAVSEKAGH QTSAESWGTG RALARIPRVG GGGTHRSGQ AAFGNMCRSG RMFAPTKTWR KWHVKVNQNE KRYAISSAVA ASGVPSLLLA RGHRIEEIPE VPLVVDDAVQ S FQKTKEAV ...String:
MAAARPTVSI YSKDGSVSSE TIALPFVFKA PIRPDLVRSV HTAVAKNKRQ PYAVSEKAGH QTSAESWGTG RALARIPRVG GGGTHRSGQ AAFGNMCRSG RMFAPTKTWR KWHVKVNQNE KRYAISSAVA ASGVPSLLLA RGHRIEEIPE VPLVVDDAVQ S FQKTKEAV ALLKEIKAYR DVVKVANSRK LRAGKGKLRN RRHVQRRGPL VVFNEDAGIV KAFRNIPGVE IVNVRRLNLL QL APGGHLG RFVIWTKSAF GLLDSVFGST TEAAQLKKNY FLPENIISNA DVTRLINSDE IQSIVKAAGP SRVKRAHVQK KNP LKNKAV LARLNPYAKA YKANVKLNTG KTPKAAGEKF LTVLHEN

UniProtKB: Large ribosomal subunit protein uL4A

+
Macromolecule #9: Large ribosomal subunit protein uL18B

MacromoleculeName: Large ribosomal subunit protein uL18B / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 33.538898 KDa
SequenceString: MPFIKAVKSS PYFSRYQTKY RRRREGKTDY YARKRLIAQA KNKYNAPKYR LVVRFSNRFV TCQIVSSRVN GDYVLAHAHS SELPRYGIK WGLANWTAAY ATGLLVARRA LAKVGLADKY EGVTEPEGEF ELTEAIEDGP RPFKVFLDVG LKRTSTGSRV F GAMKGASD ...String:
MPFIKAVKSS PYFSRYQTKY RRRREGKTDY YARKRLIAQA KNKYNAPKYR LVVRFSNRFV TCQIVSSRVN GDYVLAHAHS SELPRYGIK WGLANWTAAY ATGLLVARRA LAKVGLADKY EGVTEPEGEF ELTEAIEDGP RPFKVFLDVG LKRTSTGSRV F GAMKGASD GGLFIPHSPN RFPGFDIETE ELDDETLRKY IYGGHVAEYM EMLIDDDEER YQKQFSGLIA DGIESDQLED IY AEAYAKI REDPSFQKSG KDAAAFKAES LKYTQRKLTA EERKERFNAK VIEAGRA

UniProtKB: Large ribosomal subunit protein uL18B

+
Macromolecule #10: Large ribosomal subunit protein eL6

MacromoleculeName: Large ribosomal subunit protein eL6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 21.285996 KDa
SequenceString: MSTVKVNGAK NGGERMVLPA GEAAAKYYPA YRENVPKKAR KAVRPTKLRA SLAPGTVCIL LAGRFRGKRV VVLSQLEDTL VVTGPYKVN GVPIRRVNHR YVIATSAPKI DVSGVSVEKF TKAYFAKQKR SGPVKKDEAF FAENAPKNAL PAERIADQKA V DAKLLPAI ...String:
MSTVKVNGAK NGGERMVLPA GEAAAKYYPA YRENVPKKAR KAVRPTKLRA SLAPGTVCIL LAGRFRGKRV VVLSQLEDTL VVTGPYKVN GVPIRRVNHR YVIATSAPKI DVSGVSVEKF TKAYFAKQKR SGPVKKDEAF FAENAPKNAL PAERIADQKA V DAKLLPAI KAIPNMKEYL AASFALSNGD RPHLMKF

UniProtKB: Large ribosomal subunit protein eL6

+
Macromolecule #11: Large ribosomal subunit protein uL30C

MacromoleculeName: Large ribosomal subunit protein uL30C / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 28.780457 KDa
SequenceString: MAVASSTVPS KEQIFAPESL LKKKKTQEQS REQRVAAAAE KKAAQQKKRE LIAKRAESYD AEYRKAEREQ IELGRKARAE GNYYVPDET KLVFVIRIRG INNIPPKARK IMQLLRLIQI NNGVFVKFNK ATKEMLQVVE PYVTYGIPNL KTVRELLYKR G FGKVNKQR ...String:
MAVASSTVPS KEQIFAPESL LKKKKTQEQS REQRVAAAAE KKAAQQKKRE LIAKRAESYD AEYRKAEREQ IELGRKARAE GNYYVPDET KLVFVIRIRG INNIPPKARK IMQLLRLIQI NNGVFVKFNK ATKEMLQVVE PYVTYGIPNL KTVRELLYKR G FGKVNKQR IALSDNAIIE AALGKYSILS IEDLIHEIYT VGPNFKQAAN FIWPFQLSSP LGGWRDRKFK HFIEGGDAGK RD EHINSLV RKML

UniProtKB: Large ribosomal subunit protein uL30C

+
Macromolecule #12: Large ribosomal subunit protein eL8

MacromoleculeName: Large ribosomal subunit protein eL8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 28.659777 KDa
SequenceString: MAPKSKKVAP SPFAQPKAAK TTKNPLFVSR PRSFGIGQDI QPKRDLSRFV KWPEYIRLQR RRKILNLRLK VPPAIAQFQK TLDKNTATQ VFKLLNKYRP ETAAEKKQRL VAEAEAVANG KSAQDVSKKP YNVKYGLNHV VALIEAKKAK LVLIASDVDP I ELVVFLPA ...String:
MAPKSKKVAP SPFAQPKAAK TTKNPLFVSR PRSFGIGQDI QPKRDLSRFV KWPEYIRLQR RRKILNLRLK VPPAIAQFQK TLDKNTATQ VFKLLNKYRP ETAAEKKQRL VAEAEAVANG KSAQDVSKKP YNVKYGLNHV VALIEAKKAK LVLIASDVDP I ELVVFLPA LCKKMGVPYA IVKNKARLGT VIHQKTAAVL AVTEVREEDK NELASIVSAV DANFSAKYDE SRRKWGGGIM GG KTQALLA KRAKAAAATV RL

UniProtKB: Large ribosomal subunit protein eL8

+
Macromolecule #13: Large ribosomal subunit protein uL6B

MacromoleculeName: Large ribosomal subunit protein uL6B / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 21.413887 KDa
SequenceString:
MGRDIYKDET LTIPEGVSVD IKARLVTVKG PRGVLKQNLR RVDIELKKQG NTIKFIVWHG SRKHNACIRT AYSIINNMII GVTQGFRYK MRLVYAHFPI NINLTENGTV VEIRNFLGER ITRVIKCLPG VTVSISSAVK DEIIIEGNSL ENVSQSAANI K QICNVRNK DIRKFLDGIY VSERGNIEEL E

UniProtKB: Large ribosomal subunit protein uL6B

+
Macromolecule #14: Large ribosomal subunit protein uL16A

MacromoleculeName: Large ribosomal subunit protein uL16A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 25.421596 KDa
SequenceString: MARRPARCYR YCKNKPYPKS RYNRAVPDSK IRIFDLGRKR AGVDEFPLCI HLVSNEYEQI TSEALEAARI CANKYLVKIG GKDSFHLRV RAHPFHVVRI NKMLSCAGAD RLQTGMRHAF GKPNGLVARV NIGQILMSVR TKDSSRATAI EALRRCQYKF P GQQRIIVS ...String:
MARRPARCYR YCKNKPYPKS RYNRAVPDSK IRIFDLGRKR AGVDEFPLCI HLVSNEYEQI TSEALEAARI CANKYLVKIG GKDSFHLRV RAHPFHVVRI NKMLSCAGAD RLQTGMRHAF GKPNGLVARV NIGQILMSVR TKDSSRATAI EALRRCQYKF P GQQRIIVS KKWGFSQYAR DEYIEKRSRG EIIPDGCYAK FLNKRGSLQE KLDLFPEASF NLA

UniProtKB: Large ribosomal subunit protein uL16A

+
Macromolecule #15: Large ribosomal subunit protein uL5A

MacromoleculeName: Large ribosomal subunit protein uL5A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 19.928182 KDa
SequenceString:
MAEKAQNPMK ELRISKLVLN ISLGESGDRL TRAAKVLEQL SGQTPVFSKA RYTIRRFGIR RNEKIACHVT VRGPKAEEIL ERGLKVKEY ELKKRNFSAT GNFGFGIQEH IDLGIKYDPS IGIYGMDFYV VMDRPGMRVA RRKAQRGRVG YTHKINAEDT I NWFKQKYD AVVLGK

UniProtKB: Large ribosomal subunit protein uL5A

+
Macromolecule #16: Large ribosomal subunit protein eL13

MacromoleculeName: Large ribosomal subunit protein eL13 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 23.578145 KDa
SequenceString: MAIHVKGQLP NAHFHKDWQR YVKTWFNQPG RKLRRRQARQ TKAAKIAPRP VEAIRPAVKP PTIRYNMKVR AGRGFTLEEL KAAGVSRRV ASTIGIPVDH RRRNRSEESL QRNVERIKVY LAHLIVFPRK AGQPKKGDAT DVSGAEQTDV AAVLPITQEA V EEAKPITE ...String:
MAIHVKGQLP NAHFHKDWQR YVKTWFNQPG RKLRRRQARQ TKAAKIAPRP VEAIRPAVKP PTIRYNMKVR AGRGFTLEEL KAAGVSRRV ASTIGIPVDH RRRNRSEESL QRNVERIKVY LAHLIVFPRK AGQPKKGDAT DVSGAEQTDV AAVLPITQEA V EEAKPITE EAKNFNAFST LSNERAYARY AGARAAFQKK RAEEAEAKKK

UniProtKB: Large ribosomal subunit protein eL13

+
Macromolecule #17: Large ribosomal subunit protein eL14

MacromoleculeName: Large ribosomal subunit protein eL14 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 15.241078 KDa
SequenceString:
MEGFKRYVEV GRVVLVTKGE YTGKLAVIVD IVDHKRALID SPCSEFPRQV IRYGSVVLTH IVMKLPRGAR SGIVAKKWKA QDVCNKWAS SAWAKKLEAK KVRSQLNDFD RFAVMRLKKQ RREQVNVAVA KALKA

UniProtKB: Large ribosomal subunit protein eL14

+
Macromolecule #18: Large ribosomal subunit protein eL15B

MacromoleculeName: Large ribosomal subunit protein eL15B / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 23.878609 KDa
SequenceString: MGAYKYLEEL AKKKQSDVNL FLSRVRAWEY RQMNVIHRAS RPSRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGQT YGKPVHQGV NHLKYQRSAR CTAEERVGRY CSNLRVLNSY WVNQDATYKF FEVILVDPSH KAIRRDPRIN WIVNPVHKHR E SRGLTSIG ...String:
MGAYKYLEEL AKKKQSDVNL FLSRVRAWEY RQMNVIHRAS RPSRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGQT YGKPVHQGV NHLKYQRSAR CTAEERVGRY CSNLRVLNSY WVNQDATYKF FEVILVDPSH KAIRRDPRIN WIVNPVHKHR E SRGLTSIG KKSRGIGKGH RYNNSPQHAT WLRHNTLSLR RYR

UniProtKB: Large ribosomal subunit protein eL15B

+
Macromolecule #19: Large ribosomal subunit protein uL13A

MacromoleculeName: Large ribosomal subunit protein uL13A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 22.050088 KDa
SequenceString: MSEFQKVVVI DAKGHLLGRL ASVVAKQLLG GQKVVVVRCE ELNISGHFFR NKLKYLAYLR KACRYNPSRG AFHFRAPSRI FQKAVRGML PHKTARGQAA LEHLQAVEGI PPPFDKQKRV VVPAALRVLR LKPGRKYCTV GRLSSEVGWK YSDIVSKLEE R RKVKSAAF ...String:
MSEFQKVVVI DAKGHLLGRL ASVVAKQLLG GQKVVVVRCE ELNISGHFFR NKLKYLAYLR KACRYNPSRG AFHFRAPSRI FQKAVRGML PHKTARGQAA LEHLQAVEGI PPPFDKQKRV VVPAALRVLR LKPGRKYCTV GRLSSEVGWK YSDIVSKLEE R RKVKSAAF YQAKLAKQKK IASAKAASSV NGKLAEFGY

UniProtKB: Large ribosomal subunit protein uL13A

+
Macromolecule #20: Large ribosomal subunit protein uL22A

MacromoleculeName: Large ribosomal subunit protein uL22A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 20.854113 KDa
SequenceString:
MVRYSASPAL ETKCAKARGA YLRTHFKNSR EVAFTINGMS LKKAFIFLDN VKEHKQAVPF RRFNGGVGRT AQGKEFGVTQ ARWPVKSVK FFYDLLKNAE ANAEAKGLDM DKLIIKHVQV NAAPKQRRRT YRAHGRVTAY LSSPSHIEII VAEEEEAVPK A NDTVSRVS LKQGAKARNL AARKAITAA

UniProtKB: Large ribosomal subunit protein uL22A

+
Macromolecule #21: Large ribosomal subunit protein eL18B

MacromoleculeName: Large ribosomal subunit protein eL18B / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 21.234957 KDa
SequenceString:
MGIDIERHHV RKSQRSKPAS ENVYLKLLVK LYRFLARRTD SRFNKAILKR LFQSKTNRPP ISISKIAALT SRKSASLEGK TTVIVGTVT DDERLLTVPK LSVAALRFTK SARARILKAG GEVLTLDQLA LRAPTGSNTV LLRGKKHARE AYRHFGFGPH K HKAPYVRS EGRKFERARG RRKSRAFKV

UniProtKB: Large ribosomal subunit protein eL18B

+
Macromolecule #22: Large ribosomal subunit protein eL19B

MacromoleculeName: Large ribosomal subunit protein eL19B / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 22.813801 KDa
SequenceString: MANLRTQKRL AASVLKCGKR KVWMDPNEIS EISNANSRQN IRKLVKDGLV IRKPNLMHSR FRIRKTHAAK RLGRHTGYGK RKGTAEARM PSTVVWMRRQ RVLRRLLRKY RESGKIDKHL YHTLYLEAKG NTFKHKRALI EHIQRAKAEA NRTKLIQEQQ D ARRARAKA ...String:
MANLRTQKRL AASVLKCGKR KVWMDPNEIS EISNANSRQN IRKLVKDGLV IRKPNLMHSR FRIRKTHAAK RLGRHTGYGK RKGTAEARM PSTVVWMRRQ RVLRRLLRKY RESGKIDKHL YHTLYLEAKG NTFKHKRALI EHIQRAKAEA NRTKLIQEQQ D ARRARAKA ARQRRAKAVE EKREQIYTAA EKIEE

UniProtKB: Large ribosomal subunit protein eL19B

+
Macromolecule #23: Large ribosomal subunit protein eL20A

MacromoleculeName: Large ribosomal subunit protein eL20A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 20.642348 KDa
SequenceString:
MALKEYQVVG RKVPTEHEPV PKLFRMRLFA PNESVAKSRY WYFLKMINKV KKATGEIVAI NEISEPKPLK AKVFGIWIRY DSRSGTHNM YKEFRDTTRV GAVEAMYADM AARHRARFRS IRILKVVEVE KKEDVRRNYV KQLLNPHLKF PLPHRRTGVV G LAGKKVFA PHRPSTFY

UniProtKB: Large ribosomal subunit protein eL20A

+
Macromolecule #24: Large ribosomal subunit protein eL21B

MacromoleculeName: Large ribosomal subunit protein eL21B / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 18.39017 KDa
SequenceString:
MPHSYGIRAR TRYTFQRGFR EHGQIRLSTY LKTYKVGDIV DIKVNGAVQK GMPHKYYHGK TGVVYNVTQS SVGVLIYKVV GNRYMEKRV NVRIEHVKHS KCRQDFLDRV KANEAKRKEA KAQGKTVQLR RQPAPPATAH FVSTENNEPV TLHPVAYDTT I

UniProtKB: Large ribosomal subunit protein eL21B

+
Macromolecule #25: Large ribosomal subunit protein eL22

MacromoleculeName: Large ribosomal subunit protein eL22 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 13.344412 KDa
SequenceString:
MVKKNTKVSN KYIIDATAAV NDKIFDVAAF EKYLIDRIKV DGKTGNLGSS VVVSREGSSK IAVIAHIDFS GRYLKYLTKK FLKKHSLRD WLRVVSTKKG VYELRYYNVV VGNDEEEQ

UniProtKB: Large ribosomal subunit protein eL22

+
Macromolecule #26: Large ribosomal subunit protein uL14B

MacromoleculeName: Large ribosomal subunit protein uL14B / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 14.905514 KDa
SequenceString:
MSRGRGAASG TKYRMTLGLP VQAIMNCADN SGAKNLYIVS VFGTGARLNR LPAASCGDMV LATVKKGKPD LRKKIMPAIV VRQRKAWRR KDGVYLYFED NAGVIVNPKG EMKGSAITGP VAKECADLWP RIASNAGTVV

UniProtKB: Large ribosomal subunit protein uL14B

+
Macromolecule #27: Large ribosomal subunit protein eL24B

MacromoleculeName: Large ribosomal subunit protein eL24B / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 16.957908 KDa
SequenceString:
MKVEVCSFSG SKVYPGAGRL FVRGDNKVFR FVNKKSESLF LQRKNPRRLS WTVLYRRMHK KGISEEHAKK RTRRTVKHQR GIVGANLDV IKEKRNQRPE VRAAARAAAL KQRKDKRAAS ESEKKAIKAK SAASSARGQA IKNAKVAARR

UniProtKB: Large ribosomal subunit protein eL24B

+
Macromolecule #28: Large ribosomal subunit protein uL23A

MacromoleculeName: Large ribosomal subunit protein uL23A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 15.870718 KDa
SequenceString:
MSVAKAKGAQ KTVQKGIHNK VAKKVRTSTT FRRPKTLQLS RKPKYARKSV AHAPRLDEYK IIVNPINSES AMKKIEDDNT LVFHVHLKA NKFTIKEAVR KLYSVEPVKI NTLIRPNGTK KAFVKLSADA DALDVANRIG FL

UniProtKB: Large ribosomal subunit protein uL23A

+
Macromolecule #29: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 14.375912 KDa
SequenceString:
MKFSRDVTSS RRKQRKAHFG APSSVRRVLM SAPLSKELRE QYKIRSLPVR RDDQITVIRG SNKGREGKIT SVYRKKFLLL IERVTREKA NGASAPVGID ASKVVITKLH LDKDRKDLIV RKGGKVE

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #30: Large ribosomal subunit protein eL27A

MacromoleculeName: Large ribosomal subunit protein eL27A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 15.421304 KDa
SequenceString:
MVKILKPGKV ALITRGRFAG KKVVILQAID QGSKSHPFGH AVVAGVERYP LKVTKSMGAK RIARRSRVKP FIKVVNYNHL MPTRYALEL DNLKGLITAD TFKEPTQRSA ARKTVKKTFE EKYQSGKSAW FFTPLRF

UniProtKB: Large ribosomal subunit protein eL27A

+
Macromolecule #31: Large ribosomal subunit protein uL15B

MacromoleculeName: Large ribosomal subunit protein uL15B / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 16.723518 KDa
SequenceString:
MPTHTSKTRK LRGHVSAGHG RIGKHRKHPG GRGKAGGLQH LRSHFDKYHP GYFGKVGMRR FHLMKNPLWR PTVNLDRLWT LVPNETREK YLGKNTEVAP VINVLQSGYG KVLGKGRLPD TPVIIQTRYV SRRAEEKIKQ AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15B

+
Macromolecule #32: Large ribosomal subunit protein eL29

MacromoleculeName: Large ribosomal subunit protein eL29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 7.224249 KDa
SequenceString:
MAKSKNHTNH NQNKKAHRNG IKRPQKHRYD SLKYRDAKFR RNQKFANRGT VEAIRQAKAS A

UniProtKB: Large ribosomal subunit protein eL29

+
Macromolecule #33: Large ribosomal subunit protein eL30A

MacromoleculeName: Large ribosomal subunit protein eL30A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 11.610639 KDa
SequenceString:
MASVVTKKSK KSGDTINAKL ALTMKSGKYV LGYKSTLKTL RSGKAKLILI AGNCPPLRKS ELEYYAMLSK ANVHHYAGTN IDLGTACGK LFRVGVLAIT DAGDSDILDA

UniProtKB: Large ribosomal subunit protein eL30A

+
Macromolecule #34: Large ribosomal subunit protein eL31

MacromoleculeName: Large ribosomal subunit protein eL31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 13.290468 KDa
SequenceString:
MANTKKSAIN QVVTRDYTIH MHKRLYGVSF KKRAPRAIKE IVAFAQKHMQ TKEVRVDPSL NKEVWKRGIR NVPHRLRLRL SRKRSDEDD KALYTYVQAV DVANPKMETT VVEE

UniProtKB: Large ribosomal subunit protein eL31

+
Macromolecule #35: Large ribosomal subunit protein eL32A

MacromoleculeName: Large ribosomal subunit protein eL32A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 14.491099 KDa
SequenceString:
MAAVNIIKKR TKPFKRHQSD RFKRVGESWR KPRGIDSCVR RRFRGTISMP KIGYGNNKKT RYCMPNGLKA FLVRNVSDVE LLLMHNKTY AAEIAGNVSA RKRVEIVEKA RALGVKVTNA GAKVRSQE

UniProtKB: Large ribosomal subunit protein eL32A

+
Macromolecule #36: Large ribosomal subunit protein eL33A

MacromoleculeName: Large ribosomal subunit protein eL33A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 12.212304 KDa
SequenceString:
MPAQGHRLYV KAKHLSFQRS KHVIHPGTSL VKIEGCDSKE EAQFYLGKRI CFVYKSNKPV RGSKIRVIWG TVSRPHGNSG VVRARFTHN LPPKTFGASL RVMLYPSNV

UniProtKB: Large ribosomal subunit protein eL33A

+
Macromolecule #37: Large ribosomal subunit protein eL34B

MacromoleculeName: Large ribosomal subunit protein eL34B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 12.828181 KDa
SequenceString:
MAQRVTYRRR LAYNTRSNKT RIIKTPGNNI RYLHIKKLGT IPRCGDTGVP LQGIPALRPR EFARLSHNQK KVQRAYGGCL SANAVKDRI VRAFLIEEQK IVKQKLKQMS QK

UniProtKB: Large ribosomal subunit protein eL34B

+
Macromolecule #38: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 14.333901 KDa
SequenceString:
MALKTFELRK QSQENLAEQL QELRQELASL RVQKIAGGSG SKLSKIKTTR KDIARILTVI NESNRLAARE AYKNKKYIPL DLRQKKTRA IRRALTPYEQ SRKTLKQIKK ERYFPLRKYA LKA

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #39: Large ribosomal subunit protein eL36B

MacromoleculeName: Large ribosomal subunit protein eL36B / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 11.287438 KDa
SequenceString:
MAPGLVVGLN KGKTLTKRQL PERPSRRKGH LSKRTAFVRS IVREVAGFAP YERRVMELIR NSQDKRARKL AKKRLGTLKR AKGKIEELT SVIQSSRLAH

UniProtKB: Large ribosomal subunit protein eL36B

+
Macromolecule #40: Large ribosomal subunit protein eL37B

MacromoleculeName: Large ribosomal subunit protein eL37B / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 10.112824 KDa
SequenceString:
MTKGTQSFGM RHNKSHTICR RCGKRSFHIQ KSTCACCGYP AAKTRSYNWG AKAKRRRTTG TGRMSYLKKV HRSFKNGFRS GKPAAAVAA SA

UniProtKB: Large ribosomal subunit protein eL37B

+
Macromolecule #41: Large ribosomal subunit protein eL38A

MacromoleculeName: Large ribosomal subunit protein eL38A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 8.511081 KDa
SequenceString:
MPRQISDIKQ FLEIARRKDA TSARIKKNTN KDVKFKLRCS KYLYTLVVAD AKKAEKLRQS LPPDLTVTEV GKKA

UniProtKB: Large ribosomal subunit protein eL38A

+
Macromolecule #42: Large ribosomal subunit protein eL39

MacromoleculeName: Large ribosomal subunit protein eL39 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 6.326514 KDa
SequenceString:
MPSHKSFRTK QKLAKAARQN RPIPQWIRLR TGNTVHYNMK RRHWRRTKLN I

UniProtKB: Large ribosomal subunit protein eL39

+
Macromolecule #43: Large ribosomal subunit protein eL28

MacromoleculeName: Large ribosomal subunit protein eL28 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 14.781037 KDa
SequenceString:
MSVSNDLIWQ VIRDNNRFLV KRPEFGGIQF NREPVNVSGK NAQRFSGLCN DKAVGVQANS PRGVVLITKT NPKNAQKPAK LFRKDVIAN ASSRKTYKSI AGRIGRTGYR DDLVKVSVAR ASAILSSQRP KKTVA

UniProtKB: Large ribosomal subunit protein eL28

+
Macromolecule #3: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 1.1298282499999999 MDa
SequenceString: AUUUGACCUC AAAUCAGGUA GGACUACGCG CUGAACUUAA GCAUAUCAAU AAGCGCAGGA AAAGAAAAUA ACCAUGAUUC CCUCAGUAA CGGCGAGUGA AGCGGGAAAA GCUCAAAUUU GAAAUCUGGC AACAUUUCUU UUGUUGUCCG AGUUGUAAUU U CAAGAAGC ...String:
AUUUGACCUC AAAUCAGGUA GGACUACGCG CUGAACUUAA GCAUAUCAAU AAGCGCAGGA AAAGAAAAUA ACCAUGAUUC CCUCAGUAA CGGCGAGUGA AGCGGGAAAA GCUCAAAUUU GAAAUCUGGC AACAUUUCUU UUGUUGUCCG AGUUGUAAUU U CAAGAAGC UGCUUUGAGU GUAGACGAUC GGUCUAAGUU CCUUGGAACA GGACGUCAGA GAGGGUGAGA ACCCCGUCUU UG GUCGAUU GGAUAUGCCA UAUAAAGCGC UUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAAUG GGUGGUAAAU UUC AUCUAA AGCUAAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUAGAG UGAUCGAAAG AUGAAAAGAA CUUUGAAAAG AGAG UUAAA UAGUACGUGA AAUUGCUGAA AGGGAAGCAU UGGAAAUCAG UCUUACCUGG GUGAGAUCAG UAGUCUCUUC GCGAG ACUA UGCACUCUGA ACCUGUGGUA GGUCAGCAUC AGUUUUCGGG GGCGGAAAAA GAAUAAGGGA AGGUGGCUUU CCGGGU UCU GCCUGGGGAG UGUUUAUAGC CCUUGUUGUA AUACGUCCAC UGGGGACUGA GGACUGCGGC UUCGUGCCAA GGAUGCU GA CAUAAUGGUU UUCAAUGGCC CGUCUUGAAA CACGGACCAA GGAGUCUAGC AUCUAUGCGA GUGUUUGGGU GAUGAAAA C CCAUCCGCGA AAUGAAAGUG AAUGCAGGUG GGAACGCCCU UGUGGCGUGC ACCAUCGACC GACCCGGAAG UUUGUCAAU GGAAGGGUUU GAGUAAGAGC AUAGCUGUUG GGACCCGAAA GAUGGUGAAC UAUGCCUGAA UAGGGUGAAG CCAGAGGAAA CUCUGGUGG AGGCUCGUAG AGAUUCUGAC GUGCAAAUCG AUCUUCAAAU UUGGGUAUAG GGGCGAAAGA CUAAUCGAAC C AUCUAGUA GCUGGUUCCU GCCGAAGUUU CCCUCAGGAU AGCAGAAACU CAGAUCAGUU UUAUGAGGUA AAGCGAAUGA UU AGAGGUC UUGGGGAAGG AAUUUCCUCA ACCUAUUCUC AAACUUUAAA UAUGUAAGAC GCCCUUGUCG CUUAAUUGGA CGU GGGCCA UCGAAUGAGA GUUUCUAGUG GGCCAUUUUU GGUAAGCAGA ACUGGCGAUG CGGGAUGAAC CGAACGUGAG GUUA AGGUG CCGGAAUGUA CGCUCAUCAG ACACCAGAAA AGGUGUUAGU UCAUCUAGAC AGCAGGACGG UGGCCAUGGA AGUCG GAAU CCGCUAAGGA GUGUGUAACA ACUCACCUGC CGAAUGAACU AGCCCUGAAA AUGGAUGGCG CUUAAGCGUA CUACCC AUA CCUCACCGUC UGGGUUAGCU UUGAGAAGCU CAGACGAGUA GGCAGGCGUG GAGGUUUGUG ACGAAGCCUU GGGCGUG AG CCUGGGUCGA ACAGCCUCUA GUGCAGAUCU UGGUGGAAGU AGCAAAUAUU CAAAUGAGAA CUUUGAAGAC UGAAGUGG G GAAAGGUUCC AUGUGAACAG CAGUUGGACA UGGGUUAGUC GAUCCUAAGA GAUAGGGAAG CUCCGUAUGA AAGUUGCAC GAUUUUUCGU GCCUCCUAUC GAAAGGGAAU CCGGUUAAUA UUCCGGAACC AGAAGGUGGA AUCAACACGG CAACGUAAAU GAAGUUGGA GACGUCGGCG GGAGCCCUGG GAAGAGUUCU CUUUUCUUUU UAACAAACCA UUGAACUACC CUGAAAUCGG U UUAUCCGG AGCUAGGGUA UGGUGUUUGG AAGAGUUCAG CGCCUCAUGC UGAAUCCGGU GCGCUCUCGA CGGCCCUUGA AA AUCCAAC GGAAGAAUGG ACCUUCGGGU CCUUGUUUUC ACAUCUGGUC GUACUCAUAA CCGCAGCAGG UCUCCAAGGU GAA CAGCCU CUAGUUGAUA GAACAAUGUA GAUAAGGGAA GUCGGCAAAA UGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUA AGGGU UGGGUACGUU GGGCCUUGGA ACCUGAACGG UUGCUGGACU GAGCGUGGAC CGAUGUCUUU UCUCGCCUUU CGGGG UGAG AAGGGAUGUU GGACCUGCUU GGACCUUGGC GGCCGGGAAG UCCUUGGUCG GGCUUUUCUC CUUCUCGGGG AUUAUG CUC UUACUGGCGU ACGUUUAACA ACCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCA UU GCGAUGGCCA GAAAGUGGUG UUGACGCAAU GUGAUUUCUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCA A GCGCGGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAUC UAACUAGUGA CGCGCAUGA AUGGAUUAAC GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCUGGGGA ACGGGCCAGG CAAAAUCAGC GGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGGAUA AGUGGGAGUA U GUUUCGGC AUACGCCGGU GAAAUACCAC UACCUUUAUC GUUUCUUUAC UUAAUCAAUG AAGCGGAAUU GGGAUUUAUU UC CCAUAUU CUAGCGUUAA AGUUUCUUCG CGAACUGAUC CGCGUUGAUG ACAUUGUCAG GUGGGGAGUU UGGCUGGGGC GGC ACAUCU GUUAAAAGAU AACGCAGGUG UCCUAAGGGG GACUCAUCGA GAACAGAAAU CUCGAGUAGA AUAAAAGGGU AAAA GUCCC CUUGAUUUUG AUUUUCAGUG UGAAUACAAA CCAUGAAAGU GUGGCCUAUC GAUCCUUUGU UCCCUCGAAA UUUGA GGAC AGAGGUGCCA GAAAAGUUAC CACAGGGAUA ACUGGCUUGU GGCAGCCAAG CGUUCAUAGC GACGUUGCUU UUUGAU UCU UCGAUGUCGG CUCUUCCUAU CAUACCGAAG CAGAAUUCGG UAAGCGUUGG AUUGUUCACC CACUAAUAGG GAACGUG AG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUACCCUA CUGAUGAAGU GUCGUCGCAA UGGUAAUUCA ACUUAGUA C GAGAGGAACC GUUGAUUCAG AUCAUUGGUA UUUGCGGCUG CCUGACAAGG CAAUGCCGCG GAGCUAUCAU CUGCCGGAU AACGGCUGAA CGCCUCUAAG CCAGAAUCCG UGCCAGAAAG CGACGAUUUU UUGGUCCGCA UGAUUUAUAU GUAUAAAAAU AGAGGUAGG ACUUGUUCCU ACUCUCCUGU AUCGUAGAAG AUGGGCGAUG GUUGAUGAAA CGGAAGUGUU UUAUUGACUU G UCCAUGAA AUUCCAUUGA AAUCUUGUGC GGAAUCGAAU CCAUUGCAUA CGACUUUAAU GUGGAACGGG GUAUUGUAAG CA GUAGAGU AGCCUUGUUG UUACGAUCUG CUGAGAUUAA GCCUUUGUUC CCAAGAUUUG U

GENBANK: GENBANK: CU329672.1

+
Macromolecule #4: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 78.518867 KDa
SequenceString: UUAAACGUCU ACGGCCAUAC CUAGGCGAAA ACACCAGUUC CCGUCCGAUC ACUGCAGUUA AGCGUCUGAG GGCCUCGUUA GUACUAUGG UUGGAGACAA CAUGGGAAUC CGGGGUGCUG UAGGCUUCCU UUUAUUCUUU UUGCUUUUUG UUUGAGCUCA U UUGGGAAU ...String:
UUAAACGUCU ACGGCCAUAC CUAGGCGAAA ACACCAGUUC CCGUCCGAUC ACUGCAGUUA AGCGUCUGAG GGCCUCGUUA GUACUAUGG UUGGAGACAA CAUGGGAAUC CGGGGUGCUG UAGGCUUCCU UUUAUUCUUU UUGCUUUUUG UUUGAGCUCA U UUGGGAAU AAUAAUCAUU UACCUUGAUU CGUACUUAUU CAUUUCAAUA CCUUUGCACA UAAUAUUAUU GUGUACUAUG AA UGAUAU

GENBANK: GENBANK: CU329671.1

+
Macromolecule #5: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 52.880188 KDa
SequenceString:
AUUAUUUAAA ACUUUCAGCA ACGGAUCUCU UGGCUCUCGC AUCGAUGAAG AACGCAGCGA AAUGCGAUAC GUAAUGUGAA UUGCAGAAU UCCGUGAAUC AUCGAAUCUU UGAACGCACA UUGCGCCUUU GGGUUCUACC AAAGGCAUGC CUGUUUGAGU G UCAUU

GENBANK: GENBANK: Z19578.1

+
Macromolecule #44: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 44 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 97 / Formula: MG
Molecular weightTheoretical: 24.305 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.25 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 0.2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: OTHER / Details: Ab initio
Final reconstructionResolution.type: BY AUTHOR / Resolution: 1.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 820453
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-9axu:
Non-translating S. pombe ribosome large subunit

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more