EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for substrate binding and selection by human mitochondrial RNA polymerase.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 7134, Year 2024
掲載日	2024年8月20日
著者	Karl Herbine / Ashok R Nayak / Dmitry Temiakov /
PubMed 要旨	The mechanism by which RNAP selects cognate substrates and discriminates between deoxy and ribonucleotides is of fundamental importance to the fidelity of transcription. Here, we present cryo-EM ...The mechanism by which RNAP selects cognate substrates and discriminates between deoxy and ribonucleotides is of fundamental importance to the fidelity of transcription. Here, we present cryo-EM structures of human mitochondrial transcription elongation complexes that reveal substrate ATP bound in Entry and Insertion Sites. In the Entry Site, the substrate binds along the O helix of the fingers domain of mtRNAP but does not interact with the templating DNA base. Interactions between RNAP and the triphosphate moiety of the NTP in the Entry Site ensure discrimination against nucleosides and their diphosphate and monophosphate derivatives but not against non-cognate rNTPs and dNTPs. Closing of the fingers domain over the catalytic site results in delivery of both the templating DNA base and the substrate into the Insertion Site and recruitment of the catalytic magnesium ions. The cryo-EM data also reveal a conformation adopted by mtRNAP to reject a non-cognate substrate from its active site. Our findings establish a structural basis for substrate binding and suggest a unified mechanism of NTP selection for single-subunit RNAPs.
リンク	Nat Commun / PubMed:39164235 / PubMed Central
手法	EM (単粒子)
解像度	2.54 - 2.9 Å
構造データ	42027 8u8u EMDB-42027, PDB-8u8u: Cryo-EM Structure of Cognate Substrate ATP Bound in the Entry Site (ES) of Human Mitochondrial Transcription Elongation Complex 手法: EM (単粒子) / 解像度: 2.9 Å 42028 8u8v EMDB-42028, PDB-8u8v: Cryo-EM structure of Substrate ATP Bound in the Insertion Site (IS) of Human Mitochondrial Transcription Elongation Complex 手法: EM (単粒子) / 解像度: 2.74 Å 44448 9bdc EMDB-44448, PDB-9bdc: Cryo-EM Structure of the TEFM bound Human Mitochondrial Transcription Elongation Complex in a Closed Fingers Domain Conformation 手法: EM (単粒子) / 解像度: 2.54 Å 44449 9bdd EMDB-44449, PDB-9bdd: Cryo-EM Structure of Non-Cognate Substrate Bound in the Entry Site (ES) of Human Mitochondrial Transcription Elongation Complex 手法: EM (単粒子) / 解像度: 2.86 Å
化合物	ChemComp-APC: DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / α,β-メチレンATP / AMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	TRANSCRIPTION/DNA/RNA / Mitochondrial RNA Polymerase / Nucleotide Selection / Nucleotide Discrimination / TRANSCRIPTION / Protein-RNA-DNA Complex / POLRMT / TRANSCRIPTION-DNA-RNA complex / TEFM