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TitleStructures and Protein Engineering of the alpha-Keto Acid C-Methyltransferases SgvM and MrsA for Rational Substrate Transfer.
Journal, issue, pagesChembiochem, Vol. 25, Page e202400258-e202400258, Year 2024
Publish dateNov 15, 2023 (structure data deposition date)
AuthorsSommer-Kamann, C. / Breiltgens, J. / Zou, Z. / Gerhardt, S. / Saleem-Batcha, R. / Kemper, F. / Einsle, O. / Andexer, J.N. / Muller, M.
External linksChembiochem / PubMed:38887142
MethodsX-ray diffraction
Resolution1.54 - 2.27 Å
Structure data

PDB-8r4z:
Crystal structure of alpha keto acid C-methyl-transferases MrsA native-form
Method: X-RAY DIFFRACTION / Resolution: 1.54 Å

PDB-8rpr:
Crystal Structure of SgvM methyltransferase in complex with alpha-ketoleucine and Zn2+ ion
Method: X-RAY DIFFRACTION / Resolution: 2.14 Å

PDB-8rvc:
Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine
Method: X-RAY DIFFRACTION / Resolution: 1.969 Å

PDB-8rvs:
Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to SAM
Method: X-RAY DIFFRACTION / Resolution: 1.632 Å

PDB-8rwm:
Crystal structure of selenomethionine derivatized alpha keto acid C-methyl-transferases MrsA
Method: X-RAY DIFFRACTION / Resolution: 1.644 Å

PDB-8rww:
Crystal structure of native alpha-keto C-methyl transferase SgvM bound to ketoleucine
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-8rxf:
Crystal structure of S-SAD phased alpha-keto C-methyl transferase SgvM bound to ketoleucine
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

PDB-8rxg:
Crystal structure of alpha-keto C-methyl transferase SgvM bound to SAM
Method: X-RAY DIFFRACTION / Resolution: 1.813 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-COI:
2-OXO-4-METHYLPENTANOIC ACID

ChemComp-CL:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-NWG:
5-[(diaminomethylidene)amino]-2-oxopentanoic acid

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NA:
Unknown entry

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-GOL:
GLYCEROL

Source
  • pseudomonas syringae (bacteria)
  • streptomyces griseoviridis (bacteria)
KeywordsTRANSFERASE / S adenosylmethionine-dependent methyltransferases / biocatalysis / C-alkylation / asymmetric methylation / mutagenesis / complex / Methylransferase

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