EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the multi-subunit chloroplast RNA polymerase.
ジャーナル・号・ページ	Mol Cell, Vol. 84, Issue 5, Page 910-925.e5, Year 2024
掲載日	2024年3月7日
著者	Paula F V do Prado / Frederik M Ahrens / Monique Liebers / Noah Ditz / Hans-Peter Braun / Thomas Pfannschmidt / Hauke S Hillen /
PubMed 要旨	Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase ...Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.
リンク	Mol Cell / PubMed:38428434
手法	EM (単粒子)
解像度	2.83 - 3.52 Å
構造データ	18496 8qma EMDB-18496, PDB-8qma: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18499: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map A 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-18500: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map B. 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-18501: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map C 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-18502: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map D 手法: EM (単粒子) / 解像度: 3.34 Å EMDB-18503: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba.- Map E 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-18504: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map F 手法: EM (単粒子) / 解像度: 2.83 Å
化合物	ChemComp-FE: Unknown entry ChemComp-ZN: Unknown entry ChemComp-SAM: S-ADENOSYLMETHIONINE
由来	sinapis alba (シロガラシ)
キーワード	TRANSCRIPTION / Chloroplasts / Gene Expression / RNA / Polymerase