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- EMDB-18501: Structure of the plastid-encoded RNA polymerase complex (PEP) fro... -
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Open data
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Basic information
Entry | ![]() | ||||||||||||||||||
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Title | Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map C | ||||||||||||||||||
![]() | Map C, sharpened. | ||||||||||||||||||
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![]() | Transcription / Chloroplasts / Gene Expression / RNA / Polymerase | ||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.52 Å | ||||||||||||||||||
![]() | do Prado PFV / Ahrens FM / Pfannschmidt T / Hillen HS | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of chloroplast transcription. Authors: do Prado PFV / Ahrens FM / Ditz N / Braun H-P / Pfannschmidt T / Hillen HS | ||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 306.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.2 KB 19.2 KB | Display Display | ![]() |
Images | ![]() | 18.4 KB | ||
Masks | ![]() | 325 MB | ![]() | |
Filedesc metadata | ![]() | 4.2 KB | ||
Others | ![]() ![]() ![]() | 163.3 MB 301.3 MB 301.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 17 KB | Display | |
Data in CIF | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Map C, sharpened. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.05 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Map C, unsharpened.
File | emd_18501_additional_1.map | ||||||||||||
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Annotation | Map C, unsharpened. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Map C, half map A.
File | emd_18501_half_map_1.map | ||||||||||||
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Annotation | Map C, half map A. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Map C, half map B.
File | emd_18501_half_map_2.map | ||||||||||||
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Annotation | Map C, half map B. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Plastid-encoded DNA-dependent RNA polymerase (PEP)
Entire | Name: Plastid-encoded DNA-dependent RNA polymerase (PEP) |
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Components |
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-Supramolecule #1: Plastid-encoded DNA-dependent RNA polymerase (PEP)
Supramolecule | Name: Plastid-encoded DNA-dependent RNA polymerase (PEP) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#17 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 1 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.6 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average exposure time: 2.7 sec. / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.52 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 46170 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |