EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y.
ジャーナル・号・ページ	Commun Chem, Vol. 7, Issue 1, Page 14, Year 2024
掲載日	2024年1月17日
著者	Luka Kavčič / Andreja Kežar / Neža Koritnik / Magda Tušek Žnidarič / Tajda Klobučar / Žiga Vičič / Franci Merzel / Ellie Holden / Justin L P Benesch / Marjetka Podobnik /
PubMed 要旨	The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid ...The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid nanoparticles. However, the potential of CPs of the wide-spread flexuous filamentous plant viruses remains to be explored. Here, we show that we can control the shape, size, RNA encapsidation ability, symmetry, stability and surface functionalization of nanoparticles through structure-based design of CP from potato virus Y (PVY). We provide high-resolution insight into CP-based self-assemblies, ranging from large polymorphic or monomorphic filaments to smaller annular, cubic or spherical particles. Furthermore, we show that we can prevent CP self-assembly in bacteria by fusion with a cleavable protein, enabling controlled nanoparticle formation in vitro. Understanding the remarkable structural diversity of PVY CP not only provides possibilities for the production of biodegradable nanoparticles, but may also advance future studies of CP's polymorphism in a biological context.
リンク	Commun Chem / PubMed:38233506 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	2.41 - 12.75 Å
構造データ	17046 8opa EMDB-17046, PDB-8opa: Virus-like Particle based on PVY coat protein with stacked-ring architecture 手法: EM (らせん対称) / 解像度: 3.34 Å 17047 8opb EMDB-17047, PDB-8opb: Virus-like Particle based on PVY coat protein with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 3.58 Å 17048 8opc EMDB-17048, PDB-8opc: Virus-like Particle based on PVY coat protein with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 2.99 Å 17049 8opd EMDB-17049, PDB-8opd: Virus-like Particle based on PVY coat protein with dC40 deletion with stacked-ring architecture 手法: EM (らせん対称) / 解像度: 3.5 Å 17050 8ope EMDB-17050, PDB-8ope: Virus-like Particle based on PVY coat protein with dC40 deletion with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 3.09 Å 17051 8opf EMDB-17051, PDB-8opf: Virus-like Particle based on PVY coat protein with dC60 deletion with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 2.83 Å 17052 8opg EMDB-17052, PDB-8opg: Virus-like Particle based on PVY coat protein with dC79 deletion with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 3.2 Å 17053 8oph EMDB-17053, PDB-8oph: Head-to-tail double ring assembly from truncated PVY coat protein 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-17054: RNA-free helical assembly from truncated PVY coat protein 手法: EM (らせん対称) / 解像度: 3.59 Å EMDB-17055: Stacked-ring assembly from truncated PVY coat protein 手法: EM (単粒子) / 解像度: 3.62 Å EMDB-17056: Stacked-ring assembly from truncated PVY coat protein with L99C mutation 手法: EM (らせん対称) / 解像度: 3.45 Å EMDB-17057: Helical assembly from truncated PVY coat protein with L99C mutation 手法: EM (らせん対称) / 解像度: 3.06 Å EMDB-17058: Helical assembly from truncated PVY coat protein with K153E mutation 手法: EM (らせん対称) / 解像度: 3.41 Å EMDB-17059: Cross-shaped junction assembly from truncated PVY coat protein with E150C mutation 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-17060: Global refinement map of head-to-head double ring assembly from truncated PVY coat protein with K176E mutation 手法: EM (単粒子) / 解像度: 4.56 Å EMDB-17061: Local refinement map of head-to-head double ring assembly from truncated PVY coat protein with K176E mutation 手法: EM (単粒子) / 解像度: 3.82 Å 17062 8opj EMDB-17062, PDB-8opj: Global refinement of cubic assembly from truncated PVY coat protein with K176C mutation 手法: EM (単粒子) / 解像度: 2.99 Å 17063 8opk EMDB-17063, PDB-8opk: Local refinement of cubic assembly from truncated PVY coat protein with K176C mutation 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-17064: Spherical assembly from truncated PVY coat protein with K177E mutation 手法: EM (単粒子) / 解像度: 12.75 Å EMDB-17065: Cubic assembly from truncated PVY coat protein with K176C mutation and removed His tag 手法: EM (単粒子) / 解像度: 4.16 Å EMDB-17066: Virus-like Particle based on PVY coat protein with L99C and K176C mutation with stacked-ring architecture 手法: EM (らせん対称) / 解像度: 3.18 Å EMDB-17067: Virus-like Particle based on PVY coat protein with L99C and K176C mutation with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 3.2 Å EMDB-17068: Virus-like Particle based on PVY coat protein with E150C and G193C mutation with stacked-ring architecture 手法: EM (らせん対称) / 解像度: 3.75 Å EMDB-17069: Virus-like Particle based on PVY coat protein with E150C and G193C mutation with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 3.15 Å EMDB-17070: Virus-like Particle based on PVY coat protein with S39C and E72C mutation with stacked-ring architecture 手法: EM (らせん対称) / 解像度: 3.83 Å EMDB-17071: Virus-like Particle based on PVY coat protein with S39C and E72C mutation with RNA-free helical architecture 手法: EM (らせん対称) / 解像度: 3.69 Å 17072 8opl EMDB-17072, PDB-8opl: Virus-like Particle based on PVY coat protein with T43C and D136C mutation with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 2.41 Å EMDB-17073: Virus-like Particle based on PVY coat protein with D136C mutation with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 3.46 Å EMDB-17074: Virus-like Particle based on PVY coat protein with R46A mutation with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 2.62 Å EMDB-17075: Virus-like Particle based on PVY coat protein with E139A mutation with helical architecture encapsidating ssRNA 手法: EM (らせん対称) / 解像度: 3.17 Å
由来	potato virus y strain ntn (ウイルス)
キーワード	VIRUS LIKE PARTICLE / stacked-ring / VLP / Potyvirus / PVY / helical / RNA-free / ssRNA / dC40 / dC60 / dC79 / H2T double ring / trCP / cubes / K176C