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-Structure paper
タイトル | Structural landscape of the respiratory syncytial virus nucleocapsids. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 5732, Year 2023 |
掲載日 | 2023年9月15日 |
著者 | Lorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche / |
PubMed 要旨 | Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation. |
リンク | Nat Commun / PubMed:37714861 / PubMed Central |
手法 | EM (らせん対称) / EM (単粒子) |
解像度 | 2.8 - 6.2 Å |
構造データ | EMDB-17030: Helical nucleocapsid of the Respiratory Syncytial Virus EMDB-17031, PDB-8oou: EMDB-17034: Helical nucleocapsid of the N1-370 mutant of the human Respiratory Syncytial Virus EMDB-17035, PDB-8op1: EMDB-17036: Double-headed nucleocapsid of the human Respiratory Syncytial Virus EMDB-17037: Ring-capped nucleocapsid of the Respiratory Syncytial Virus EMDB-17038, PDB-8op2: |
由来 |
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キーワード | VIRAL PROTEIN / D10-symmetry / N-RNA / nucleoprotein / RSV / nucleocapsid |