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Structure paper

TitleStructural basis for abscisic acid efflux mediated by ABCG25 in Arabidopsis thaliana.
Journal, issue, pagesNat Plants, Vol. 9, Issue 10, Page 1697-1708, Year 2023
Publish dateSep 4, 2023
AuthorsWei Ying / Lianghuan Liao / Hong Wei / Yongxiang Gao / Xin Liu / Linfeng Sun /
PubMed AbstractAbscisic acid (ABA) is a phytohormone essential to the regulation of numerous aspects of plant growth and development. The cellular level of ABA is critical to its signalling and is determined by its ...Abscisic acid (ABA) is a phytohormone essential to the regulation of numerous aspects of plant growth and development. The cellular level of ABA is critical to its signalling and is determined by its rate of biosynthesis, catabolism and the rates of ABA transport. ABCG25 in Arabidopsis thaliana has been identified to be an ABA exporter and play roles in regulating stomatal closure and seed germination. However, its ABA transport mechanism remains unknown. Here we report the structures of ABCG25 under different states using cryo-electron microscopy single particle analysis: the apo state and ABA-bound state of the wild-type ABCG25 and the ATP-bound state of the ATPase catalytic mutant. ABCG25 forms a homodimer. ABA binds to a cone-shaped, cytosolic-facing cavity formed in the middle of the transmembrane domains. Key residues in ABA binding are identified and verified by a cell-based ABA transport assay. ATP binding leads to closing of the nucleotide-binding domains of opposing monomers and conformational transitions of the transmembrane domains. Together, these results provide insights into the substrate recognition and transport mechanisms of ABCG25 in Arabidopsis, and facilitate our understanding of the ABA transport and signalling pathway in plants.
External linksNat Plants / PubMed:37666962 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.7 Å
Structure data

35768

8iwj

EMDB-35768, PDB-8iwj:
ABCG25 Wild Type in Apo-state
Method: EM (single particle) / Resolution: 3.0 Å

35769

8iwk

EMDB-35769, PDB-8iwk:
ABCG25 Wild Type purified with DDM plus CHS in ABA-bound state
Method: EM (single particle) / Resolution: 3.0 Å

35774

8iwn

EMDB-35774, PDB-8iwn:
ABCG25 EQ mutant in ATP-bound state
Method: EM (single particle) / Resolution: 3.0 Å

36780

8k0x

EMDB-36780, PDB-8k0x:
ABCG25 Wild Type purified with DDM in the ABA-bound state
Method: EM (single particle) / Resolution: 3.2 Å

36781

8k0z

EMDB-36781, PDB-8k0z:
ABCG25 Wild Type purified with DDM in the apo-state
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-A8S:
(2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid / hormone*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / ABA / ABCG / plant hormone

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