EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4396, Year 2023
掲載日	2023年7月20日
著者	Xiang Feng / Michelle M Spiering / Ruda de Luna Almeida Santos / Stephen J Benkovic / Huilin Li /
PubMed 要旨	The T4 bacteriophage gp41 helicase and gp61 primase assemble into a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication. How the primosome is assembled and how the primer ...The T4 bacteriophage gp41 helicase and gp61 primase assemble into a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication. How the primosome is assembled and how the primer length is defined are unclear. Here we report a series of cryo-EM structures of T4 primosome assembly intermediates. We show that gp41 alone is an open spiral, and ssDNA binding triggers a large-scale scissor-like conformational change that drives the ring closure and activates the helicase. Helicase activation exposes a cryptic hydrophobic surface to recruit the gp61 primase. The primase binds the helicase in a bipartite mode in which the N-terminal Zn-binding domain and the C-terminal RNA polymerase domain each contain a helicase-interacting motif that bind to separate gp41 N-terminal hairpin dimers, leading to the assembly of one primase on the helicase hexamer. Our study reveals the T4 primosome assembly process and sheds light on the RNA primer synthesis mechanism.
リンク	Nat Commun / PubMed:37474605 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 5.7 Å
構造データ	27707 8dtp EMDB-27707, PDB-8dtp: Close state of T4 bacteriophage gp41 hexamer bound with single strand DNA 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-27708: Closed conformation of bacteriophage T4 helicase hexamer with single strand DNA but no primase added. 手法: EM (単粒子) / 解像度: 3.4 Å 27719 8due EMDB-27719, PDB-8due: Open state of T4 bacteriophage gp41 hexamer bound with single strand DNA 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-27720: Open coformation of bacteriophage T4 helicase hexamer around single strand DNA without primase added 手法: EM (単粒子) / 解像度: 3.5 Å 27724 8duo EMDB-27724, PDB-8duo: DNA-free T4 Bacteriophage gp41 hexamer 手法: EM (単粒子) / 解像度: 5.7 Å 27737 8dvf EMDB-27737, PDB-8dvf: T4 Bacteriophage primosome with single strand DNA, state 1 手法: EM (単粒子) / 解像度: 3.3 Å 27739 8dvi EMDB-27739, PDB-8dvi: T4 bacteriophage primosome with single strand DNA, State 2 手法: EM (単粒子) / 解像度: 3.2 Å 27751 8dw6 EMDB-27751, PDB-8dw6: T4 bacteriophage primosome with single-strand DNA, State 3 手法: EM (単粒子) / 解像度: 3.5 Å 27756 8dwj EMDB-27756, PDB-8dwj: Primase of mutant bacteriophage T4 primosome with single strand DNA/RNA primer hybrid in primer exiting state 手法: EM (単粒子) / 解像度: 3.9 Å 29658 8g0z EMDB-29658, PDB-8g0z: Mutant bacteriophage T4 gp41 helicase hexamer bound with single strand DNA and ATPgammaS in the stalled primosome 手法: EM (単粒子) / 解像度: 3.61 Å EMDB-29744: local refinement of the primase/DNA/primer region in mutant T4 primosome 手法: EM (単粒子) / 解像度: 4.1 Å 29902 8gao EMDB-29902, PDB-8gao: bacteriophage T4 stalled primosome with mutant gp41-E227Q 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN*: Unknown entry
由来	escherichia phage t4 (ファージ) synthetic construct (人工物)
キーワード	REPLICATION/DNA/RNA / phage / complex / helicase / REPLICATION-DNA-RNA complex / REPLICATION