EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into human co-transcriptional capping.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 14, Page 2464-22477.e5, Year 2023
掲載日	2023年7月20日
著者	Gaurika Garg / Christian Dienemann / Lucas Farnung / Juliane Schwarz / Andreas Linden / Henning Urlaub / Patrick Cramer /
PubMed 要旨	Co-transcriptional capping of the nascent pre-mRNA 5' end prevents degradation of RNA polymerase (Pol) II transcripts and suppresses the innate immune response. Here, we provide mechanistic insights ...Co-transcriptional capping of the nascent pre-mRNA 5' end prevents degradation of RNA polymerase (Pol) II transcripts and suppresses the innate immune response. Here, we provide mechanistic insights into the three major steps of human co-transcriptional pre-mRNA capping based on six different cryoelectron microscopy (cryo-EM) structures. The human mRNA capping enzyme, RNGTT, first docks to the Pol II stalk to position its triphosphatase domain near the RNA exit site. The capping enzyme then moves onto the Pol II surface, and its guanylyltransferase receives the pre-mRNA 5'-diphosphate end. Addition of a GMP moiety can occur when the RNA is ∼22 nt long, sufficient to reach the active site of the guanylyltransferase. For subsequent cap(1) methylation, the methyltransferase CMTR1 binds the Pol II stalk and can receive RNA after it is grown to ∼29 nt in length. The observed rearrangements of capping factors on the Pol II surface may be triggered by the completion of catalytic reaction steps and are accommodated by domain movements in the elongation factor DRB sensitivity-inducing factor (DSIF).
リンク	Mol Cell / PubMed:37369200
手法	EM (単粒子)
解像度	3.2 - 4.0 Å
構造データ	17403 8p4a EMDB-17403, PDB-8p4a: Structural insights into human co-transcriptional capping - structure 1 手法: EM (単粒子) / 解像度: 3.6 Å 17404 8p4b EMDB-17404, PDB-8p4b: Structural insights into human co-transcriptional capping - structure 2 手法: EM (単粒子) / 解像度: 3.2 Å 17405 8p4c EMDB-17405, PDB-8p4c: Structural insights into human co-transcriptional capping - structure 3 手法: EM (単粒子) / 解像度: 3.8 Å 17406 8p4d EMDB-17406, PDB-8p4d: Structural insights into human co-transcriptional capping - structure 4 手法: EM (単粒子) / 解像度: 3.6 Å 17407 8p4e EMDB-17407, PDB-8p4e: Structural insights into human co-transcriptional capping - structure 5 手法: EM (単粒子) / 解像度: 3.9 Å 17408 8p4f EMDB-17408, PDB-8p4f: Structural insights into human co-transcriptional capping - structure 6 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-MGT: 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE / 7,8-ジヒドロ-7-メチルグアノシン5′-三りん酸 ChemComp-SAM: S-ADENOSYLMETHIONINE
由来	homo sapiens (ヒト) sus scrofa (ブタ) unidentified (未定義) synthetic construct (人工物)
キーワード	TRANSCRIPTION / rna polymerase II / capping