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-Structure paper
Title | Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from . |
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Journal, issue, pages | Front Plant Sci, Vol. 14, Page 1139106, Year 2023 |
Publish date | Mar 21, 2023 |
Authors | Xu Zhang / Zixuan Zhou / Lin Dai / Yulin Chao / Zheng Liu / Mingdong Huang / Qianhui Qu / Zhonghui Lin / |
PubMed Abstract | Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB ...Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB complex to catalyze HJ branch migration. (, Pa) is a ubiquitous opportunistic bacterial pathogen that can cause serious infection in a variety of host species, including vertebrate animals, insects and plants. Here, we describe the cryo-Electron Microscopy (cryo-EM) structure of the RuvAB-HJ intermediate complex from . The structure shows that two RuvA tetramers sandwich HJ at the junction center and disrupt base pairs at the branch points of RuvB-free HJ arms. Eight RuvB subunits are recruited by the RuvA octameric core and form two open-rings to encircle two opposite HJ arms. Each RuvB subunit individually binds a RuvA domain III. The four RuvB subunits within the ring display distinct subdomain conformations, and two of them engage the central DNA duplex at both strands with their C-terminal β-hairpins. Together with the biochemical analyses, our structure implicates a potential mechanism of RuvB motor assembly onto HJ DNA. |
External links | Front Plant Sci / PubMed:37025142 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.16 - 7.02 Å |
Structure data | EMDB-33013, PDB-7x5a: EMDB-33043, PDB-7x7p: EMDB-33044, PDB-7x7q: PDB-7x5b: |
Chemicals | ChemComp-ADP: ChemComp-HOH: |
Source |
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Keywords | DNA BINDING PROTEIN/DNA / RuvA / Holliday junction / Homologous recombination / DNA damage repair / DNA BINDING PROTEIN-DNA COMPLEX / MOTOR PROTEIN / RuvB / Holliday juncition / ATP hydrolysis / MOTOR PROTEIN/DNA / MOTOR PROTEIN-DNA COMPLEX / RECOMBINATION/DNA / Branch migration / RECOMBINATION-DNA COMPLEX |