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Structure paper

TitleStructure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
Journal, issue, pagesNature, Vol. 614, Issue 7949, Page 788-793, Year 2023
Publish dateFeb 15, 2023
AuthorsMinrui Fan / Jianxiu Zhang / Chien-Ling Lee / Jinru Zhang / Liang Feng /
PubMed AbstractThe sodium-chloride cotransporter (NCC) is critical for kidney physiology. The NCC has a major role in salt reabsorption in the distal convoluted tubule of the nephron, and mutations in the NCC cause ...The sodium-chloride cotransporter (NCC) is critical for kidney physiology. The NCC has a major role in salt reabsorption in the distal convoluted tubule of the nephron, and mutations in the NCC cause the salt-wasting disease Gitelman syndrome. As a key player in salt handling, the NCC regulates blood pressure and is the target of thiazide diuretics, which have been widely prescribed as first-line medications to treat hypertension for more than 60 years. Here we determined the structures of human NCC alone and in complex with a commonly used thiazide diuretic using cryo-electron microscopy. These structures, together with functional studies, reveal major conformational states of the NCC and an intriguing regulatory mechanism. They also illuminate how thiazide diuretics specifically interact with the NCC and inhibit its transport function. Our results provide critical insights for understanding the Na-Cl cotransport mechanism of the NCC, and they establish a framework for future drug design and for interpreting disease-related mutations.
External linksNature / PubMed:36792826 / PubMed Central
MethodsEM (single particle)
Resolution2.81 - 3.04 Å
Structure data

29096

8fhn

EMDB-29096, PDB-8fhn:
Cryo-EM structure of human NCC (class 2)
Method: EM (single particle) / Resolution: 3.0 Å

29097

8fho

EMDB-29097, PDB-8fho:
Cryo-EM structure of human NCC (class 1)
Method: EM (single particle) / Resolution: 2.95 Å

29098

8fhp

EMDB-29098, PDB-8fhp:
Cryo-EM structure of human NCC (class 3-1)
Method: EM (single particle) / Resolution: 3.04 Å

29099

8fhq

EMDB-29099, PDB-8fhq:
Cryo-EM structure of human NCC (class 3-2)
Method: EM (single particle) / Resolution: 2.81 Å

29100

8fhr

EMDB-29100, PDB-8fhr:
Cryo-EM structure of human NCC (class 3-3)
Method: EM (single particle) / Resolution: 2.9 Å

29103

8fht

EMDB-29103, PDB-8fht:
Cryo-EM structure of human NCC
Method: EM (single particle) / Resolution: 3.02 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-XZF:
Polythiazide

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

Source
  • homo sapiens (human)
  • danio rerio (zebrafish)
KeywordsMEMBRANE PROTEIN / Membrane transporter

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