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TitleWatson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases.
Journal, issue, pagesMbio, Vol. 14, Page e0258722-e0258722, Year 2023
Publish dateOct 23, 2020 (structure data deposition date)
AuthorsSmiley, A.T. / Tompkins, K.J. / Pawlak, M.R. / Krueger, A.J. / Evans 3rd, R.L. / Shi, K. / Aihara, H. / Gordon, W.R.
External linksMbio / PubMed:36541758
MethodsX-ray diffraction
Resolution1.3 - 1.73 Å
Structure data

PDB-7kii:
Muscovy duck circovirus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7kij:
Muscovy duck circovirus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site
Method: X-RAY DIFFRACTION / Resolution: 1.69 Å

PDB-7kik:
Wheat dwarf virus Rep domain circular permutation complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+
Method: X-RAY DIFFRACTION / Resolution: 1.73 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • muscovy duck circovirus
  • wheat dwarf virus
KeywordsREPLICATION/DNA / HUH-tag / HUH motif / Rep domain / viral protein / single stranded DNA / ssDNA / ssDNA binding / REPLICATION / DNA BINDING PROTEIN / REPLICATION-DNA complex

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